ccpp, ys, pgi final

8/8/2019 Ccpp, Ys, Pgi Final

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2/23

Overview

About CCPP

Etiology

Epidemiology

Differential Diagnosis

Preventive measures

Actions to Take


3/23

W

hat is CCPP ?

Bou fila

Extremely Contagious & frequently fatal

Innaive flocks, extensive morbidity, mortality

Enlisted asTYPE- B by OIE


4/23

Etiological agent

M. capricolum sscapripneumoniae(Mycoplasma F-38)

M. mycoides cluster

M. capricolum ss.capricolum

M. mycoides sscapri M. mycoides ssmycoides LC type

M. mycoides ssmycoides SC type

Mycoplasma sp. bovine group 7


5/23

Epidemiological features

Captive wild goats

Sheep & Cattle not affected

Chronic & latent CarriersShed more organisms

Stress, close contact , over crowding,confinement, climate changes,

PPR & Capri pox viruses act as predisposing factor

More outbreaks after heavy rains in India


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Africa

Far East

Middle east

Eastern Europe

Former USSR

Asia (India & Pakistan)

Never reported in North America

Distribution


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Countries involved

Confirmed by

isolationof

mycoplasma

Clinical disease reported or

suspected

Africa Chad, Eritrea, Ethiopia,Kenya, Niger, Sudan, Tunisia,

Uganda

Algeria,Burkina Faso,Benin, Cameroon,

Central African Republic, Djibouti,

Egypt, Libya, Mali, Nigeria, Somali, Zaire

Asia Nepal, Oman, United ArabEmirates,

Turkey,Yemen

Afghanistan,Bangladesh,India, Iran, Iraq,

Israel, Jordan, Kuwait, Lebanon,

Pakistan, Saudi Arabia, Syria


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Prevalence in India

No isolationofMccp

(However, F-38 from cattle with mastitis)

Strong serological evidences

Different states


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Differential Diagnosis

Clinical Picture

High fever, lethargy, anorexia Coughing, labored breathings

Reluctant to move

Nasal discharge

Stringy saliva

Abortion

Death, 7-10 days


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Post Mortem Lesions

Lungs & Pleura(usually unilateral)

Granular lungappearance

Fibrinous pneumonia


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O

ther Lesions

Straw-colored fluid in thorax

Pea-sized yellow nodules

Adhesions tochest wall

Thickened pulmonary pleura


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Sampling

Before collecting or sending any samples,theproper authorities should be contacted

Samples should only be sent under secureconditions to authorized laboratories


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15/23

Molecular techniques

PCR based on 16S rRNA

detects allM. mycoides cluster

Sequencing & REA of PCR productswith PstI detectsM. ccp specifically

PCRcan detectMccp directlyfrom pleural fluid or lung and

also from pleural fluiddried on filter paper


16/23

Criteria for CCPP

IsolationofM. c. capripneumoniae

or strong serological evidence ordetection by PCR-REA

Lesions restricted to lung and pleuraconsisting a pleuropneumonia

Highly contagious with highmorbidity/mortality

No enlargement of interlobular

septa of lung


17/23

Preventive measures

Recommended Actions

Notificationof Authorities Area Veterinarian In-Charge

State Veterinary Authority

Testing,slaughter

Quarantine Desirable for infected flocks

Strict, due to communicability

Disinfection


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Antibiotic therapy

Tetracycline, tylosin,erythromycin, streptomycin

Early intervention and treatment needed

Newly infected countries Trade, movement restrictions

Slaughter of infected animals


19/23

Vaccination

Saponin killed Vaccines available in NVI,

Ethiopia and KVVPI, Kenya Good/excellent protection

Kids above 6 months

0.2 ml I/D, Jan March

Inactivate Mccp just beforeinoculation or 12 h at 40C


20/23

Actions to take

Establishment of diagnostic lab at national level

Notification to higher authorities before collection &sending clinical samples

Sent sample under strict secure conditions & lowtemperature to authorized lab

Differentiate CCPP from Pasteurellosis &M.m.capriorM. mycoides LC infections

Use LAT withMccp for herd diagnosis


21/23

Use PCR based on 16S rRNA with REA to detectMccp.

directly from mixed cultures & clinical samples

It can also detectMccp. directly from pleural fluid dried

on filter paper

In endemic areas, do flocktesting,slaughter,on-site

quarantine of affected and vaccinationof healthy

animals before introducing new animals.

Competitive-ELISA being more specific test mustbe available commercially


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In an outbreak, flock testing followed by quarantine,

movement restrictions, slaughter of affected & contact animals,cleaning & disinfection of premises along with vaccination in

association with antibiotic therapy are better option than

antibiotic therapy alone.

Improvement of both production capacity and technology

needs for saponin inactivated CCPP vaccine used in Kenya &

Ethiopia.

Mccp screening as well as vaccination should be done

before wild goats released into zoos & other places.


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ccpp, ys, pgi final

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