CshRNA-LPSCshRNA-Unt

Figure S1

HshRNA-Unt HshRNA-LPSa

Fig. S1. (a). LPS-induced apoptosis in RAW264.7 cells. Reduced LPS-induced apoptosis in HuR knockdown stable clones. Red-TUNEL positive cells; Blue-DAPI; Purple-combined. CshRNA-Unt, Control shRNA treated RAW264.7 cells, no treatment; CshRNA-LPS, Control shRNA treated RAW264.7 cells, LPS treatment; HshRNA-Unt, HuR knock-down stable clones, no treatment; HshRNA-LPS, HuR knock-down stable clones, LPS treatment.

(Fig. S1. (b). Percent apoptotic cells averaged from ten high power visual fields (HVF). CshRNA-Unt, Control shRNA treated RAW264.7 cells, no treatment; CshRNA-LPS, Control shRNA treated RAW264.7 cells, LPS treatment; HshRNA-Unt, HuR knock-down stable clones, no treatment; HshRNA-LPS, HuR knock-down stable clones, LPS treatment. *P<0.01, untreated vs. LPS-treated groups; #P<0.01, between LPS-treated groups.

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Figure S1

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Figure S2

Fig S2. Quantitative real-time PCR analysis of HuR mRNA expression in RAW264.7 cells. Cells were treated with LPS (10ng/ml) and/or IL-10 (10ng/ml) for 2h. IL-10 inhibited LPS-mediated increases in HuR mRNA expression (*P<0.01 vs. LPS-treated cells). Values are the means ± SE from triplicate experiments.

mdm-2

p53

β-actin

β-actin

TGF-β

β-actin

Fig S3. p53, TGF-β and mdm-2 protein expression in RAW 264.7 cells. RAW264.7 cells were treated with LPS for 16hrs. Equal loading of proteins in each lane is shown by β-actin. CshRNA, Control shRNA treated RAW264.7 cells; HshRNA, HuR knock-down stable clones; Unt- untreated cells; LPS, LPS treated cells.

Figure S3

Unt LPS Unt LPS

CshRNA HshRNA