blood smear evaluation

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BLOOD SMEAR EVAL

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Page 1: Blood smear evaluation

BLOOD SMEAR EVAL

Page 2: Blood smear evaluation

Quick scan on low power through entire slide for adequate distribution of cells

Check for plt clumping & atypical cells at feathered edge. (if there is plt clumping there is no need to try and count plt in the monolayer)

FEATHERED EDGE

Page 3: Blood smear evaluation

Where the action is at: diff 40X morph 100X WBC morph, RBC morph, plt count (if no plt clumping at feathered edge)

MONOLAYER

Page 4: Blood smear evaluation

Canine-Biconcave with area of central pallor 7umFeline-no consistent central pallor, vary more in shape 6um

NORMAL RBCS

Page 5: Blood smear evaluation

Evidence of regeneration Polychromatophils-anisocytosis

nRBC (<4/100WBCs)

RBC MORPHOLOGY

Page 6: Blood smear evaluation

AUTOAGGLUTINATION

Page 7: Blood smear evaluation

Spherical, Loss normal bioconcave shape, no central pallor >5/HPF is abnormal

SPHEROCYTES

Page 8: Blood smear evaluation

Often shrinking artifact-abn lytes, uremia, rattlesnake envenoation.

CRENATION/ECCHINOCYTES

Page 9: Blood smear evaluation

Irregularly spaced membrane projections-abnormal accumulation of lipids in RBC membrane-metabolic, HAS, dz of liver, spleen and kidney

ACANTHOCYTES

Page 10: Blood smear evaluation

Fragmented by mechanical injury. DIC, microvascular hemolysis, HAS, heartworm dz. 1/ 3HPF is abnormal

SCHISTOCYTE

Page 11: Blood smear evaluation

Accumulations of damaged Hg-acetaminophen toxicosis, acute onion and zinc toxicity in dogs, DM, lymphoma in cats

HEINZ BODIES

Page 12: Blood smear evaluation

Punctate aggregates of ribosomes-from highly regenerative anemia or lead toxicosis

BASOPHILIC STIPPLING

Page 13: Blood smear evaluation

Nuclear remnants-regenerative anemia or splenic disease

HOWELL-JOLLY BODIES

Page 14: Blood smear evaluation

# per 10HPF X 15,000

PLATELETS

Page 15: Blood smear evaluation

Neutrophils-lobulated nucleus with pale to light pink/ purple cytoplasm

WBC MORPHOLOGY

Page 16: Blood smear evaluation

U or J shaped nucleus, constriction of band <1/2 width of remainder, cytoplasm similar to mature neutrophils. <5% normal.

BAND NEUTROPHIL

Page 17: Blood smear evaluation

Presence of dohle bodies, cytoplasmic basophilia (cytoplasm is more blud-purple), foamy cytoplasmic vacuolization (soap bubbles).

TOXIC CHANGES

Page 18: Blood smear evaluation

Densely staining round to oval nuclei with small amounts of cytoplasm. Reactive (antigenic stimulation) darker cytoplasm, increased size.

LYMPHOCYTES

Page 19: Blood smear evaluation

Lots of light to deep blue cytoplasm (darker then neut) with nuclei that is not round. Quite variable in appearance. Often larger then neuts and lymphs.

MONOCYTES

Page 20: Blood smear evaluation

In low numbers, slightly smaller then neuts with less lobulated nuclei, contains prominent pink granules which are rod shaped in cats.

EOSINOPHILS

Page 21: Blood smear evaluation

Largest, very uncommon, nucleus is ribbon like (not segmented) often with pink/ purple granules present.

BASOPHILS