Blood Hemolysis Detector - Ultimaker: 3D Printers User Hemolysis Detector User Manual ... Getting Started ... Microfluidic channel: polydimethylsiloxane (PDMS)

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  • Blood Hemolysis Detector

    User Manual

    Written by: Kayla Marquis, Jordan Seekins,

    Hanna Anderson, Marie-Louise Doe

    April 2017

  • 2

    Table of Contents

    OVERVIEW ................................................................................................................................................................ 3

    GETTING STARTED ................................................................................................................................................ 4

    CONTENTS IN BOX ....................................................................................................................................................... 4

    ADDITIONAL SUPPLIES OR EQUIPMENT NECESSARY FOR OPERATION ..................................................................... 5

    SETUP ......................................................................................................................................................................... 6

    MICROFLUIDICS SETUP ............................................................................................................................................... 6

    OPTICAL UNIT SETUP ................................................................................................................................................. 6

    LABVIEW CALIBRATION ............................................................................................................................................ 7

    PHONE APPLICATION SET-UP .................................................................................................................................... 7

    OPERATION .............................................................................................................................................................. 9

    SAMPLE SEPARATION ................................................................................................................................................. 9

    OPTICAL ANALYSIS-LABVIEW UNIT ..................................................................................................................... 11

    OPTICAL ANALYSIS-PHONE CAMERA ASSEMBLY................................................................................................... 13

    MAINTENANCE ..................................................................................................................................................... 15

    CLEANING .................................................................................................................................................................................... 15

    PART REPLACEMENT.................................................................................................................................................................. 16

    TROUBLESHOOTING .......................................................................................................................................... 16

    MICROFLUIDICS .......................................................................................................................................................................... 16

    OPTICS .......................................................................................................................................................................................... 16

    SPECIFICATIONS .................................................................................................................................................. 16

    MICROFLUIDICS .......................................................................................................................................................................... 16

    OPTICS .......................................................................................................................................................................................... 16

    SCHEMATICS ......................................................................................................................................................... 17

    OVERALL SYSTEM ....................................................................................................................................................................... 17

    MICROFLUIDIC CHANNEL .......................................................................................................................................................... 18

    CIRCUIT SCHEMATIC .................................................................................................................................................................. 18

    LABVIEW BLOCK DIAGRAM .................................................................................................................................................... 19

    BREADBOARD CIRCUIT .............................................................................................................................................................. 19

    OPTICAL ANALYSIS CHAMBER ................................................................................................................................................. 19

    PHONE FILTER ATTACHMENT .................................................................................................................................................. 20

    SAMPLE CARTRIDGE HOUSING ................................................................................................................................................. 21

    GLOSSARY OF TECHNICAL TERMS ................................................................................................................ 23

  • 3

    Overview

  • 4

    When red blood cells rupture, free hemoglobin is released. If not immediately detected,

    severe health complications such as acute kidney or heart failure could result. Traditional free

    hemoglobin detection methods require blood to be mixed with chemicals as well as the use of

    expensive detection equipment. For a fraction of the time, money, and sample size of

    conventional methods, this point-of-care hemolysis detector will indicate the concentration of

    free hemoglobin while maintaining the integrity of the blood sample.

    This hemolysis detector consists of a blood plasma separator and an optical detector. The

    plasma separator uses a microfluidic system to isolate the plasma from the red blood cells. Free

    hemoglobin is contained in the plasma, permitting efficient detection using a photoresistor and

    LED to determine the concentration of free hemoglobin in the sample. A second option for

    optical analysis for a mobile phone is also provided, where a phone analysis unit and instructions

    for the ImageJ analysis software are included.

    Getting Started

    Contents in box

    Microfluidic chamber with connected tubing and luer lock fittings

    3mL luer lock syringe

    Glass slide capillary tube

    Optical analysis chamber with cover and circuitry in breadboard

  • 5

    Hemolysis Analyser LabView VI disc

    Phone analysis unit:

    Phone filter attachment

    Sample cartridge housing

    Additional supplies or equipment necessary for operation

    Syringe pump Amazing Goop, or other waterproof sealant 70% ethanol cleaning solution Computer NI myDAQ console, terminal connector and USB cable Set of calibration slides Galaxy S7 Edge cellphone LabVIEW ImageJ Microsoft Excel or other spreadsheet program

  • 6

    Setup

    Microfluidics Setup

    1. No setup is required.

    Optical Unit Setup

    1. Attach the breadboard wires into the black NI myDAQ terminal connector by securing

    them into the indicated channels.

    Green: +15V Yellow: AGND (AO) Orange: 0+(AI +/-10V) Red: 0 (DIO 0-5V) Brown: DGND

    2. Connect the NI myDAQ console to computer.

    3. Open the LabView VI titled Hemolysis Analyzer from the disc provided.

  • 7

    LabVIEW Calibration

    1. Run LabView VI by clicking the arrow in the toolbar.

    2. Turn LED on by pressing LED On/Off button.

    For this part, disregard instrument hemolysis indications

    3. Insert 0 mg/mL calibration slide into analyser.

    4. Record the voltage shown in the Calibration Control Panel.

    5. Do this for remaining 5 calibration slides.

    6. Using Excel, plot the voltage vs. the concentration.

    7. Fit a linear trendline to the data.

    8. Input the slope and y-intercept of this line into the controls in the calibration panel.

    9. VI will now display concentration data based on the calibration line determined.

    Phone Application Set-up

    1. Slide the phone filter attachment over the top half of the phone.

  • 8

    2. Make sure the slots for the camera and flash are aligned properly.

    3. Attach the sample box to the device.

  • 9

    Operation

    Sample Separation

    1. Acquire blood sample, minimum of 100

    2. Put blood sample in 3 mL luer lock syringe

    3. Attach syringe luer lock to tubing

    4. Insert the red blood cell outlet tubing into a small collection container.

  • 10

    5. Put plasma outlet tubing into contact with glass slide capillary tube to fill with plasma.

    6. Set syringe pump flow rate to 25 mL/hr to initiate whole blood separation.

    7. Once slide reservoir is full stop syringe pump.

    8. Seal the slide assembly with waterproof sealant to prevent leakage of sample.

    9. Choose LabVIEW unit or phone camera optical analysis.

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