bioresource research collaborative group shinozaki ...shinozaki research collaborative group riken...

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Laboratory Head

Shinozaki Research Collaborative Group

Goal

Activities

Plants differ from animals in various ways and have inherent

capabilities that animals lack, such as plant-hormone-mediated

regulation, responses to various environmental stresses, light

regulation, and high-efficiency totipotency. Our research group

is studying the functions of plant genes using various mutants

(T-DNA/transposon insertional mutants and enhancer-tagged

mutants) of Arabidopsis thaliana. The group is interested in

plant genes involved in environmental stress response and

Analysis of plant genes using insertional mutants,

enhance-tagged mutants or FOX lines

Collection of plant full-length cDNAs

MembersChief Scientist, Director of Gene Discovery Research Group

Kazuo SHINOZAKI, Ph.D. (2005. 4~) (1989. 4~2005.3 Director of Plant Molecular Biology Laboratory)

Research Scientist

Taishi UMEZAWA, Ph.D. (2005. 4~)

Miki FUJITA, Ph.D. (2006. 4~) (2003.4~2006.3 CREST Research Scientist)

Takashi KUROMORI, Ph.D. (2005.4~) Fumiyoshi MYOUGA, Ph.D. (2005.4~)

Postdoctoral Researcher

Kaoru URANO, Ph. D. (2005. 4~)

Fuminori TAKAHASHI, Ph.D. (2007. 5~) (2004.4~2007.3 Junior Research Associate)

Special Postdoctral Researcher

Rie NISHIYAMA, Ph. D. (2006. 4~)

JSPS Research Fellow

Behnam Babak, Ph.D. (2007.10~)

Special Fixed Term Contract Employee

Saho MIZUKADO (2008.4~) (2006.4~2008.3 PSC Technical Staff)

Junior Research Associate

Masahide MIZOGUCHI (2007.4~)

Student Trainee

Masaki NAKAMURA (2005.4~2007.3)

1.

2.

tolerance, plant hormone response, seed development and

germination. The group aims to understand complex systems

involved in the regulation of gene expression cellular signal

transduction in response to environmental stimuli in higher

plants. The group also wants to contribute to the collection of

useful resources for basic biology and biotechnology through

their research and development.

Analysis of plant genes involved in environmental stress

response and tolerance, plant hormone response, seed

development and germination

Comprehensive analysis of plant stress response by the

“-omics” approach

3.

4.

Bioresource Research Collaborative Group

Kazuo SHINOZAKI, Ph.D.

Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ～ 2007

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Umezawa, Nishiyama, Ito, Babak, Mizukado, Kobayashi, Matsuo, Eto, UranoTakahashi, Fujita, Mizoguchi, Shinozaki, Shodai, Ozawa

Specific Aims

1. Phenome analysis of Arabidopsis genes using Ds transposon mutant collection

Using Ds-tagged Arabidopsis mutants with homozygous

knockout genes , we are analyzing gene funct ions

comprehensively. We have observed phenotypic changes of

the mutants and analyzed the function of disrupted genes.

In the course of the project, we have isolated a male sterile

mutant, male sterility1 (ms1) (Fig. 1). The MS1 gene encodes

a PHD-type transcriptional activator and regulates pleiotropic

steps such as pollen grain and tapetum development.

2. Soybean full-length cDNA projectRIKEN PSC is planning to collect large sets of full-length

cDNAs from various crops or trees. Our group constructed

a full-length enriched cDNA library from soybean, which

is the most important legume crop, as part of the project.

Approximately 40,000 cDNA clones were collected, and

Agency Staff

Hatsuyo SHODAI (2000.1~) Hiroko KOBAYASHI (2003.4~)

Kumiko OZAWA (2003.4~)

[Antibiotic Laboratory]

Senior Research Scientist

Takuya ITO, Ph.D. (2007. 4~)(2000.4~2007.3 Plant Molecular Biology Laboratory)

[Plant Molecular Biology Laboratory]

Research Scientist

Takeshi KATAGIRI, Ph.D. (2001.10~2007.3)


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some of the cDNAs were sequenced and analyzed. We are

now preparing a database system for browsing our database of

soybean full-length cDNAs (Fig.2).

3. Miniscale FOX hunting systemRecently, we developed a novel system known as full-length

cDNA over-expressor (FOX) gene hunting, which involves

the random overexpression of a normalized. Arabidopsis full-

length cDNA library. Using a miniscale version of the system,

which focused on 41 stress-inducible transcription factors, we

identified AtbZIP60, a bZIP-type transcription factor, as a salt-

tolerant gene.

4. Functional analysis of plant genes involved in environmental stress response

Plants have a complex network of signal transduction and

gene expression through plant hormones in response to

various environmental stresses, such as dehydration, high

Figure 1. Transmission electron microscopic analysis of the ms1 mutant.MI: Microspore, TA: Tapetum.(A and C) Wild type. (B and D) ms1 mutant.(Compare A and B) In the ms1 mutant, microspores (arrows) and tapetum (arrow heads) became vacuolated, and finally, no mature pollen grains were formed.(Compare C and D) In the ms1 mutant, characteristic pollen wall structure, exine, was not formed.

Figure 2. Schematic diagram of Soybean cDNA Project

Soybean RNA mixture

Full-length cDNA library

Cloning

Sequencing for 5’/3’-ends

Clustering analysis

Full-length

sequence

Agillent

oligoarray

Molecular

markers etc.

salinity and low temperature. To increase plant productivity,

our research group is conducting gene hunting and functional

analysis in relation to environmental stress response.

5. Transgenic wheat project: Toward the practical use of stress tolerant crops (Fig. 3)

To generate stress-tolerant transgenic wheat, we constructed

transformation vectors containing useful Arabidopsis drought-

tolerant genes such as AtGolS2, NCED3, and SnRK2C. In

addition, we collected 38 wheat full-length genes orthologous

to Arabidopsis genes that have been reported to function in

drought stress. Based on the information from wheat EST

pools, full-length cDNA clones, and microarray analysis,

we are identifying the wheat constitutive or drought-stress-

inducible promoters.

6. Comprehensive analysis of ABA-regulated dehydration response utilizing metabolomics and transcriptomics in Arabidopsis

In plants, adaptation to stress is a complex biological process

involving global changes in gene expression and metabolite

composition. We carried out an integrated analysis of the

metabolome and transcriptome to reveal new molecular

mechanisms of dynamic metabolic networks in response to

dehydration using a knockout mutant of the NCED3 gene

involved in the dehydration-inducible biosynthesis of ABA.


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Figure 3. Engineering environmental stress tolerant wheatWe search and select the stress-inducible promoters and functional genes for molecular breeding about drought tolerance. Based on these knowledge, we produce the stress tolerant wheat in the field.

Publications【Original Papers】 (*Peer reviewed journals)

Sakurai T., Satou M., Akiyama K., Iida K., Seki M.,

Kuromori T., Ito T., Konagaya A., Toyoda T., Shinozaki

K.: “RARGE: a large-scale database of RIKEN

Arabidopsis resources ranging from transcriptome to

phenome.” Nuc. Acid. Res. 33, D647-D650 (2005).*

Osakabe Y., Maruyama K., Seki M., Satou M., Shinozaki

K., Yamaguchi-Shinozaki K.: “An LRR receptor kinase,

RPK1, is a key membrane-bound regulator of abscisic

acid early signaling in Arabidopsis.” Plant Cell 17,

1105-1119 (2005).*

Urano K., Hobo T., Shinzoaki K.: “Arabidopsis ADC

genes involved in polyamine biosynthesis are essential

for seed development.” FEBS Lett. 579, 1557-1564

(2005).*

Katagiri T., Ishiyama K., Kato T., Tabata S., Kobayashi

M., Shinozaki K.: “An important role of phosphatidic

acid in ABA signaling during germination in Arabidopsis

thaliana.” Plant J. 43, 107-117 (2005).*

Yamada M., Morishita H., Urano K., Shinozaki N.,

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5.

Yamaguchi-Shinozaki K., Shinozaki K., Yoshiba Y.:

“Effects of free proline accumulation in petunias under

drought stress.” J. Exp. Bot. 56. 1975-1981 (2005).*

Noutoshi Y., Ito T., Seki M., Nakashita H., Yoshida S.,

Marco Y., Shirasu K., Shinozaki K.: “A single amino acid

insetion in the WRKY domain of the Arabidopsis TIR-

NBS-LRR-WRKY type disease resistance protein SLH

(SENSITIVE TO LOW HUMIDITY1) causes activation

of defense responses and hypersensitive cell death.” Plant

J. 43, 873-888 (2005).*

Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M.,

Kamiya A., Mizukado S., Sakurai T., Shinozaki K.: “A

resource of 5,814 dissociation transposon-tagged and

sequence-indexed lines of Arabidopsis transposed from

start loci on chromosome 5.” Plant Cell Physiol. 46,

1149-1153 (2005).*

Noutoshi Y., Ito T., Shinozaki K.: “ALBINO AND PALE

GREEN 10 encodes BBMII isomerase involved in

histidine biosynghesis in Arabidopsis thaliana.” Plant

Cell Physiol. 46, 1165-1172 (2005).*

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Kamei A., Seki M., Umezawa T., Ishida J., Satou M.,

Akiyama K., Zhu J-K., Shinozaki K.: “Analysis of gene

expression profiles in Arabidopsis salt overly sensitive

mutants sos2-1 and sos3-1.” Plant Cell Environ. 28,

1267-1275 (2005).*

Iida K., Seki M., Sakurai T., Satou M., Akiyama K.,

Toyoda T., Konagaya A., Shinozaki K: “RARTF:

database and tools for complete sets of Arabidopsis

transcription factors.” DNA Res. 12, 247-256 (2005).*

Oono Y., Seki M., Satou M., Iida K., Akiyama K., Sakurai

T., Fujita M., Yamaguchi-Shinozaki K., Shinozaki K.:

“Monitoring expression profiles of Arabidopsis genes

during cold acclimation and deacclimation using DNA

microarrays.” Funct. Integr. Genomics 6, 212-234

(2005).*

Fujita Y., Fujita M., Satoh R., Maruyama K., Parvez

MM., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki

K., Yamaguchi-Shinozaki K.: “AREB1 is a transcription

activator of novel ABRE-dependent ABA-signaling that

enhances drought stress tolerance in Arabidopsis.” Plant

Cell 17, 3470-3488 (2005).*

Nakashima K., Fujita Y., Katsura K., Maruyama K.,

Narusaka Y., Seki M., Shinozaki K., Yamaguchi-

Shinozaki K.: “Transcriptional Regulation of ABI3- and

ABA-responsive Genes Including RD29B and RD29A

in Seeds, Germinating Embryos, and Seedlings of

Arabidopsis.” Plant Mol. Biol. 60, 51-68 (2005).*

Nishimura N., Kitahata N., Seki M., Narusaka Y.,

Narusaka M., Kuromori T., Asami T., Shinozaki

K., Hirayama T.: “Analysis of ABA Hypersensitive

Germination2 revealed the pivotal functions of PARN

in stress response in Arabidopsis.” Plant J., 44, 972-984

(2005).*

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Ito Y., Katsura K., Maruyama K., Taji T., Kobayashi

M., Seki M., Shinozaki K. Yamaguchi-Shinozaki

K.:“Functional analysis of rice DREB1/CBF-type

transcription factors involved in cold-responsive gene

15.

expression in transgenic rice”, Plant Cell Physiol., 47,

141-153 (2006).*

Yoshida T., Nishimura N., Kitahata N., Kuromori T.,

Ito T., Asami T., Shinozaki K. Hirayama T.: “ABA

Hypersensitive Germination3 encodes a protein

phosphatase 2C (AtPP2CA) that strongly regulates

ABA signaling during germination among Arabidopsis

PP2Cs”, Plant Physiol., 140, 115-126 (2006).*

Umezawa T., Okamoto M., Kushiro T., Nambara E.,

Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y.

Shinozaki K.: “CYP707A3, a major ABA 8’-hydroxylase

involved in dehydration rehydration response in

Arabidopsis thaliana”, Plant J., 46, 171-182 (2006).*

Furihata T., Maruyama K., Fujita Y., Umezawa T.,

Yoshida R., Shinozaki K. Yamaguchi-Shinozaki K.:

“ABA-dependent multisite phosphorylation regulates the

activity of a transcription activation AREB1”, Proc. Natl.

Acad. Sci. USA., 103, 1988-1993 (2006).*

Yoshida R., Umezawa T., Mizoguchi T., Takahashi S.,

Takahashi F. Shinozaki K.: “The regulatory domain

of SRK2E/OST1/SnRK2.6 interacts with ABI1 and

integrates ABA and osmotic stress signals controlling

stomatal closure in Arabidopsis”, J. Bio. Chem., 281,

5310-5318 (2006).*

Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki

T., Aoki M., Seki E., Matsuda T., Tomo Y., Terada

T., Shirouzu M., Tanaka A., Seki M., Shinozaki K.

Yokoyama S.: “An Arabidopsis SBP-domain fragment

with a disrupted C-terminal zinc-binding site retains its

tertiary structure”, FEBS Lett., 580, 2109-2116 (2006).*

Hasegawa Y., Seki M., Mochizuki Y., Heida N., Hirosawa

K., Okamoto N., Sakurai T., Satou M., Akiyama K.,

Iida K., Lee K., Kanaya S., Demura T., Shinozaki K.,

Konagaya A. Toyoda T.: “A flexible representation of

omic knowledge for thorough analysis of microarray

data”, Plant methods, 2,5 (2006).*

Sakuma Y., Maruyama K., Osakabe Y., Qin F., Seki M.,

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Shinozaki K. Yamaguchi-Shinozaki K.:“Functional

analysis of an Arabidopsis transcription factor, DREB2A,

involved in drought-responsive gene expression”, Plant

Cell, 18,1292-1309 (2006).*

Noutoshi Y., Kuromori T., Wada T., Hirayama T., Kamiya

A., Imura Y., Yasuda M., Nakashita H., Shirasu K.

Shinzoaki K.:“Loss of NECROTIC SPOTTED LESIONS

1 associates with cell death and defense responses in

Arabidopsis thaliana”, Plant Mol. Biol., 62, 29-42

(2006).*

Kuromori T., Wada T., Kamiya A., Yuguchi M., Yokouchi

T., Imura Y., Takabe H., Sakurai T., Akiyama K.,

Hirayama T., Okada K. Shinzoaki K.:“A trial of phenome

analysis using 4000 Ds-insertional mutants in gene-

coding regions of Arabidopsis”, Plant J., 47, 640-651

(2006).*

Kitahata N., Han S-Y., Noji N., Saito T., Kobayashi

M., Nakano T., Kuchitsu K., Shinozaki K., Yoshida

S., Matsumoto S., Tsujimoto M. Asami T.:“A 9-cis-

epoxycarotenoid dioxygenase inhibitor for use in

the elucidation of abscisic acid action mechanisms”,

Bioorganic & Medicinal Chem., 15, 5555-5561 (2006).*

Nishiuchi T., Masuda D., Nakashita H., Ichimura K.,

Shinozaki K., Yoshida S., Kimura M., Yamaguchi I.

Yamaguchi K.:“Fusarium phytotoxin trichothecenes

have an elicitor-like activity in Arabidopsis thaliana, but

the activity differed significantly among their molecular

species”, Mol Plant Microve Interact., 19, 512-520

(2006).*

Machida M., Takechi K., Sato H., Chung S.J., Kuroiwa

H., Takio S., Seki M., Shinozaki K., Fujita T., Hasebe

M. Takano H.:“Gene for the peptidoglycan synthesis

pathway are essential for chloroplast division in moss”,

Proc. Natl. Acad. Sci. USA., 103, 6753-6758 (2006).*

Gross J., Cho W-K., Lezhneva L., Falk J., Krupinska

K., Shinzoaki K., Seki M., Herrmann R.G. Meurer J.:

“A plant locus essential for phylloquinone (Vitamin K1)

Biosynthesis orininated from a fusion of foure eubacterial

genes”, J. Biol. Chem., 281, 17189-17196 (2006).*

Oono Y., Seki M., Satou M., Iida K., Akiyama K.,

Sakurai T., Fujita M., Yamaguchi-Shinozaki K. Shinozaki

K.:“Monitoring expression profiles of Arabidopsis

genes during cold acclimation and deacclimation using

DNA microarrays”, Funct. Integr. Genomics, 6, 212-234

(2006).*

Myouga F., Motohashi R., Kuromori T., Nagata

N. Shinozaki K.:“An Arabidopsis chloroplast-

targeted Hsp101 homolog, APG6, has an essential

role in choloroplast development as well as heat stress

response”, Plant J., 48, 249-260 (2006).*

Sakuma Y., Maruyama K., Qin F., Osakabe Y., Shinozaki

K. Yamaguchi-Shinozaki K.: “Dual function of an

Arabidopsis transcription factor DREB2A in water-stress-

responsive and heat-stress-responsive gene expression”,

Proc. Natl. Acad. Sci. USA., 103. 18822-18827 (2006).*

Ichimura K., Casais C., Peck SC., Shinozaki K. Shirasu

K.:“MEKK1 is required for MPK4 activation and

regulates tissue specific and temperature dependent cell

death in Arabidopsis”, J. Bio. Chem., 281, 36969-36976

(2006).*

Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,

Kuroda H., Kondou Y., Tsuhara Y., Suzuki K., Ishikawa

A., Seki M., Fujita M., Motohashi R., Nagata N., Takagi

T., Shinozaki K. Matsui M.:“The FOX hunting system:

an alternative gain-of-function gene hunting technique”,

Plant J., 45, 974-986 (2006).*

Matsuda T., Kigawa T., Koshiba S., Inoue M., Aoki M.,

Yamasaki K., Seki M., Shinozaki K. Yokoyama S.: “Cell-

free synthesis of zinc-binding proteins”, J. Struct Funct

Genomics, 7, 93-100 (2006).*

Tran P. L-S., Nakashima K., Sakuma Y., Fujita Y.,

Osakabe Y., Qin F., Simpson SD., Maruyama K., Fujita Y.,

Shinozaki K. Yamaguchi-Shinozaki K.: “Co-expression

of the stress-inducible zinc finger homeodomain ZFHD1

and NAC transcription factors enhances exporession

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of the ERD1 gene in Arabidopsis”, Plant J., 49, 46-63

(2007).*

Qin F., Kakimoto M., Sakuma Y., Maruyama K., Osakabe

Y., Tran L-S., Shinzoaki K. Yamaguch-Shinozaki K.:

“Regulation and functional analysis of ZmDREB2A in

response to drought and heat stresses in Zea mays L.”,

Plant J.,50, 54-69 (2007).*

Mitsuda N.,Iwase A., Yamamoto H., Yoshida M., Seki

M., Shinozaki K. Ohme-Takagi M.: “NAC transcription

factors, NST1 and NST3, are key regulators of the

formation of secondary walls in woody tissues of

Arabidopsis”, Plant Cell, 19, 270-280 (2007).*

Takahashi F., Yoshida R., Ichimura K., Mizoguchi T., Seo

S., Yonezawa M., Maruyama K., Yamaguchi-Shinozaki

K. Shinozaki K.: “Dissecting the jasmonate signaling

pathway by a novel MAP kinase cascade, MKK3-MPK6,

in Arabidopsis”, Plant Cell, 19, 805-818 (2007).*

Itoh T., Tanaka, T., Barrero R.A., Yamasaki C., Fujii

Y., Hilton P.B., Antonio B.A., Aono H., Apweiler R.,

Bruskiewich R., Bureau T., Burr F., Fuks G., Habara T.,

Haberer G., Han B., Harada E., Hiraki A.T., Hirochika

H., Hoen D., Hokari H., Hosokawa S., Hsing Y.I., Ikawa

H., Ikeo K., Imanishi T., Ito Y., Jaiswal P., Kanno M.,

Kawahara Y., Kawamura T., Kawashima H., Khurana

J.P., Kikuchi S., , Komatsu S., Koyanagi K.O., Kubooka

H., Lieberherr D., Lin Y.C., Lonsdale D., Matsumoto

T., Matsuya A., McCombie W.R., Messing J., Miyao A.,

Mulder N., Nagamura Y., Nam J., Namiki N., Numa H.,

Nurimoto S., O'Donovan C., Ohyanagi H., Okido T., de

Oliveira A.C., Oota S., Osato N., Palmer L.E., Quetier

F., Raghuvanshi S.,Saichi N.,Sakai H., Sakai Y., Sakata

K., Sakurai T., Sato F., Sato Y., Schoof H., Seki M.,

Shibata M., Shimizu Y., Shinozaki K., Shinso Y., Singh

N.K., Smith-White B., Takeda J., Tanino M., Tatusova

T., Thongjuea S., Todokoro F., Tsugane M., Tyagi A.K.,

Vanavichit A., Wang A., Wing R.A., Yamaguchi K.,

Yamamoto M., Yamamoto N., Yu Y., Zhang H., Zhao

Q., Higo K., Burr B., Gojobori T. Sasaki T.: “Curated

Genome Annotation of Oryza sativa ssp. Japonica

and Comparative Genome Analysis with Arabidopsis

thaliana”. Genome Res. 17, 175-183 (2007).*

Nakagawa Y., Katagiri T., Shinozaki K., Qi Z., Tatsumi

H., Furuichi T., Kishigami A., Sokabe M., Kojima

I., Sato S., Kato T., Tabata S., Iida K., Terashima A.,

Nakano M., Ikeda M., Yamanaka T. Iida K.: “Arabidopsis

plasma membrane protein crucial for Ca2+ influx and

touch sensing in roots”, Proc. Natl. Acad. Sci. USA., 104,

3639-3644 (2007).*

Nakashima K., Tran P. L-S., Nguyen D.V., Fujita M.,

Maruyama K., Todaka D., Ito Y., Hayashi N., Shinozaki

K. Yamaguchi-Shinozaki K.: “Functional analysis of

a NAC-type transcription factor OsNAC6 involved in

abiotic and biotic stress-responsive gene expression in

rice”, Plant J., 51, 617-630 (2007).*

Mizuno S., Osakabe Y., Maruyama K., Ito T., Osakabe

K., Sato T., Shinozaki K. Yamaguchi-Shinozaki K.:

“Receptor-like protein kinase 2 (RPK 2) is a novel factor

controlling anther development in Arabidopsis thaliana”,

Plant J., 50, 751-766 (2007).*

Iuchi S., Koyama H., Iuchi A., Kobayashi Y., Kitabayashi

S., Kobayashi Y., Ikka T., Hirayama T., Shinozaki K.

Kobayashi M.: “Zinc finger protein STOP1 is critical

for proton tolerance in Arabidopsis and coregulates a

key gene in aluminum tolerance”, Proc. Natl. Acad. Sci.

USA., 104, 9900-9905 (2007).*

Okuda K., Myouga F., Motohashi R., Shinozaki

K. Shikanai T.: “Conserved domain structure of

pentatricopeptide repeat proteins involved in choroplast

RNA editing”, Proc. Natl. Acad. Sci. USA., 104,

8178-8183 (2007).*

Tian C., Chikayama E., Tsuboi Y., Kuromori T.,

Shinozaki K., Kikuchi J. Hirayama T.: “Top-down

phenomics of Arabidopsis thaliana-METABOLIC

PROFILING BY ONE- AND TWO-DIMENSIONAL

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

AND TRANSCRIPTOME ANALYSIS OF ALBINO

MUTANTS”, J. Bio Chem., 282, 18532-18541 (2007).*

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Nishimura N., Yoshida T., Kitahata N., Asami T.,

Shinozaki K. Hirayama T.: “ABA-Hypersensitive

Germination1 encodes a protein phosphatase 2C,

an essential component of abscisic acid signaling in

Arabidopsis seed”, Plant J., 50, 935-949 (2007).*

Motohashi R., Yamazaki T., Myouga F., Ito T., Ito

K., Satou M., Kobayashi M., Nagata N., Yoshida S.,

Nagashima A., Tanaka K., Takahashi S. Shinozaki K.:

“Chloroplast ribosome release factor 1 (AtcpRF1) is

essential for chloroplast development”, Plant Mol. Biol.,

64, 481-497 (2007).*

Fujita M, Mizukado S, Fujita Y, Ichikawa T, Nakazawa

M, Seki M, Matsui M, Yamaguchi-Shinozaki K,

Shinozaki K.: “Identification of stress-tolerance-related

transcription-factor genes via mini-scale Full-length

cDNA over-eXpressor (FOX) gene hunting system”,

Biochem. Biophys.Res.Commun. 364, 250-257 (2007).*

Ukitsu H.*, Kuromori T.*, Toyooka K., Goto Y.,

Matsuoka K., Sakuradani E., Shimizu S., Kamiya

A., Imura Y., Yuguchi M., Wada T., Hirayama T.,

Shinozaki K.　(* equally contribution) “Cytological and

Biochemical Analysis of COF1, an Arabidopsis Mutant

of an ABC Transporter Gene”. Plant Cell Physiol., 48,

1524-1533 (2007).*

Ito T., Nagata N., Yoshiba Y., Ohme-Takagi M., Ma H.

Shinozaki K.: “The Arabidopsis MALE STERILITY1

gene encoding a PHD-type transcription factor regulates

pollen and tapetum development”, Plant Cell, 19,

3549-3562 (2007).*

Miwa K., Takano J., Omori H., Seki M., Shinozaki

K. Fujiwara T.: “Plants tolerant of high boron levels”,

Science, 318, 1417 (2007).*

Tran L-S. P., Urao T., Qin F., Maruyama K., Kakimoto

T., Shinozaki K. Yamaguchi-Shinozaki K.: “Functional

analysis of AHK1/ATHK1 and cytokinin receptor

histidine kinases in response to abscisic acid, drought,

and salt stress in Arabidopsis”, Proc. Natl. Acad. Sci.

USA., 104, 20623-20628 (2007).*

Sakurai T., Plata G., Rodriguez-Zapata F., Seki M.,

Salcedo A., Toyoda A., Tohme J., Sasaki Y., Shinozaki K.

Ishitani M.: “Sequencing analysis of 20,000 full-length

cDNA clones from cassava reveals lineage specific

expansions in gene families related to stress response”,

BMC Plant Biology, 7, 66-84 (2007).*

Nanjo T., Sakurai T., Totoki Y., Toyoda A., Nishiguchi

M., Kado T., Igasaki T., Futamura N., Seki M., Sasaki

Y., Shinozaki K. Shinohara K.: “Functional annotation

of 19,841 Populus nigra full-length enriched cDNA

clones”, BMC Genomics, 8, 448-447 (2007).*

Sasaki Y., Takahashi K., Oono Y., Seki M., Yoshida R.,

Shinozaki K. Uemura M.:” Characterization of growth-

phase-specific responses to cold in Arabidopsis thaliana

suspension-cultured cells”, Plant Cell Environ., 31,

354-365 (2008).*

Sakai T., vd Honing H., Nishioka M., Uehara Y.,

Takahashi M., Fujisawa N., Saji K., Seki M., Shinozaki

K., Jones MA., Smirnoff N., Okada K. Wasteneys

GO: “Armadillo repeat-containing kinesins and a

NIMA-related kinase are required for epidermal-cell

morphogenesis in Arabidopsis”, Plant J., 53, 157-171

(2008).*

Garcia M., Myouga F., Takechi K., Sato H., Nabeshima

K., Nagata N., Takio S., Shinzoaki K. Takano H.: “An

Arabidopsis homolog of the bacterial peptidoglycan

synthesis enzyme MurE has an essential role in

chloroplast development”, Plant J., 53, 924-934 (2008).*

Yoshida T., Sakuma Y., Todaka D., Maruyama K.,

Qin F., Mizoi J., Kidokoro S., Fujita Y., Shinozaki K.

Yamaguchi- Shinozaki K.: “Functional analysis of an

Arabidopsis heat-shock transcription factor HsfA3 in

the transcriptional cascade downstream of the DREB2A

stress-regulatory system”, Biochem. Biophys. Res.

Commun., 368, 515-521 (2008).*

Kuromori T., Azumi Y., Hayakawa S., Kamiya A., Imura

Y., Wada T. Shinozaki K.: “Homologous chromosome

46.

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48.

49.

50.

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53.

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― 178 ―

【Reviews】Seki M., Ishida J., Nakajima M., Enju A., Iida K., Satou

M., Fujita M., Narusaka Y., Narusaka M., Sakurai T.,

Akiyama K., Oono Y., Kamei A., Umezawa T., Mizukado

S., Maruyama K., Yamaguchi-Shinozaki K., Shinozaki

K.: “Genomic analysis of stress response.” In Plant

Abiotic Stress. M.A. Jenks and P.M. Hasegawa, eds,

Blackwell Publishing Ltd, Sheffield, UK, 248-265 (2005).

Seki M., Satou M., Sakurai T., Akiyama K., Iida K.,

Ishida J., Nakajima M., Enju A., Narusaka M., Fujita M.,

Oono Y., Yamaguchi-Shinozaki K., Carninci P., Kawai

J., Hayashizaki Y., Shinzoaki K.: “Full-length cDNAs

for the discovery and annotation of genes in Arabidopsis

thaliana.” Plant Functional Genomics, Dario Leister eds,

Food Products Press, 3-22 (2005).

Seki M., Ishida J., Nakajima M., Enju A., Kamei A.,

Oono Y., Narusaka M., Satou M., Sakurai T., Shinozaki

K.: “Gene expression profiling in plants using cDNA

microarrays.” DNA Microarrays, Edited by Ulrike A

Nuber, Taylor & Francis, Germany, 25-38 (2005).

Yamaguchi-Shinozaki K., Shinozaki K.: “Organization

of cis-acting regulatory elements in osmotic- and cold-

stress-responsive promoters.” Trends Plant Sci. 10,

88-94. (2005).*

Yamaguchi-Shinozaki K., Shinozaki K.: “Transcriptional

regulatory networks in cellular responses and tolerance to

dehydration and cold stresses.” Annual Review of Plant

Biology, 57, 781-803 (2006).

1.

2.

3.

4.

5.

pairing is completed in crossover defective atzip4

mutant”, Biochem. Biophys.Res.Commun., 370, 98-103

(2008).*

Cartagena Joyce A., Matsunaga S., Seki M., Kurihara

D., Yokoyama M., Shinozaki K., Fujimoto S., Azumi

Y., Uchimiya S. Fukui K.: “The Arabidopsis SDG4

contributes to the regulation of pollen tube growth by

methylation of histone H3 lysines 4 and 36 in muture

pollen”, Dev. Biol., 315, 355-368 (2008).*

Shimizu H., Peng L., Myouga F., Motohashi R.,

Shinozaki K. Shikanai T.: “CRR23/NdhL is a subunit

of the chloroplast NAD(P)H dehydrogenase complex in

Arabidopsis”, Plant Cell Physiol., 49, 835-842 (2008).*

Qin F., Sakuma Y., Tran L-S., Maruyama K., Kidokoro S.,

Fujita Y., Fujita M., Umezawa T., Sawano Y., Miyazono

K., Tanokura M., Shinozaki K. Yamaguchi-Shinozaki K.:

“Arabidopsis DREB2A-interacting proteins function as

RING E3 ligases and negatively regulate plant drought

stress-responsive gene expression”, Plant Cell, 20,

1693-1707 (2008).*

Mochida K., Saisho D., Yoshida T., Sakurai T. Shinozaki

K.: “TriMEDB: A database to integrate transcribed

markers and facilitate genetic studies of the tribe

Triticeae”, BMC Plant Biol., 8, 72 (2008)

Yasuda M., Ishikawa A., Jikumaru Y., Seki M., Umezawa

T., Asami T., Maruyama-Nakashita A., Kudo T.,

Shinozaki K., Yoshida S. Nakashita H.: “Antagonistic

interaction between syntemic acquired resistance and

the abscisic acid-mediated abiotic stress response in

Arabidopsis”, Plant Cell, 20, 1678-1692 (2008).*

Goda H., Sasaki E., Akiyama K., Maruyama-Nakashita

A., Nakabayashi K., Li W., Ogawa M., Yamauchi Y.,

Prestpm J., Aoki K., Kiba T., Takatsuto S., Fujioka S.,

Asami T., Nakano T., Kato H., Mizuno T., Sakakibara

H., Yamaguchi S., Nambara E., Kamiya Y., Takahashi

H., Yokota Hirai M., Sakurai T., Shinozaki K., Saito K.,

Yoshida S. Shimada Y.: “The AtGenExpress hormone-

and chemical-treatment data set: Experimental design,

data evaluation, model data analysis, and data access”,

Plant J., 55, 526-542 (2008).*

Endo A., Sawada Y., Takahashi H., Okamoto M., Ikegami

K., Koiwai H., Seo M., Toyomasu T., Mitsuhashi W.,

Shinozaki K., Nakazono M., Kamiya Y., Koshiba

T. Nambara E.: “Drought induction of Arabidopsis

9-cis-epoxycarotenoid dioxygenase occurs in vascular

parenchyma cells”, Plant Physiol., 147, 1984-1993

(2008).*

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― 179 ―

Umezawa T., Fujita M., Fujita Y., Yamaguchi-Shinozaki

K., Shinozaki K.: “Enginieering drought tolerance in

plants: discovering and tailoring genes to unlock the

future.” Curr.Opin.Biotech., 17, 113-122 (2006).*

Shinozaki K., Yamaguchi-Shinozaki K.: “Global

analysis of gene networks to solve complex abiotic

stress responses, Cold Hardiness in Plants; Molecular

Genetics, Cell Biology and Physiology.” Proceeding

of 7th Internationa Plant Cold Hardiness Seminar in

Sapporo Japan, Edited by T.H.H. Chen, M. Uemura and S.

Fujikawa, CABI Publishing, 1-10 (2006).

Taji T., Takahashi S., Shinozaki K.: “Inositols and their

metabolites in abiotic and biotic stress responses.”

Subcellular Biochemistry Vol 39 on Biology of Inositols

and Phosphoinositides, Edetid by Arun Lahiri Majumder

and B.B. Biswas, 239-264 (2006).

Seki M., Kamiya A., Carninci P., Hayashizaki Y.,

Shinozaki K.: “Generation of full-length cDNA libraries

from plant materials.” In “Methods in Molecular

Biology-ESTs (Edited by Dr. John Parkinson).” Humana

Press Inc., NJ.in press (2006).

Fujita M., Fujita Y., Noutoshi Y., Takahashi F., Narusaka

Y., Yamaguchi-Shinzoaki K., Shinozaki K.: “Crosstalk

between abiotic and biotic stress responses: a current

view from the points of convergence in the stress

signaling networks.” Curr.Opin. Plant Biol. 9, 436-442

(2006).*

Yamaguchi-Shinozaki K., Sakuma Y., Ito Y., Shinozaki

K.: “The DRE/DREB Regulon of gene expression in

Arabidopsis and rice in response to drought and cold

stress.” Drought Adaptation in Cereals, Edted by Jean-

Marcel Ribaut, Food Products Press, 583-597 (2006).

Seki, M., Umezawa, T., Kim, J.M., Matsui, A., To,

T., Shinozaki, K.: “Transcriptome analysis of plant

drought and salt stress response., Advances in Molecular

Breeding toward Drought and Salt Tolerant Crops.”

Edited by Drs. M.A. Jenks, P.M. Hasegawa and S.M.

Jain., Springer Publishing. 261-283 (2007).

Nakashima K., Todaka D., Ahmed S., Umezawa T., Seki

M., Shinozaki K., Yamaguchi-Shinozaki K.: “Molecular

analysis of abiotic stress: Tolerant mechanisms in

soybean and its application to breeding.” JIRCAS

Working Report, 51, 13-19 (2007).

Shinozaki K., Yamaguchi-Shinozaki K.: “Gene networks

involved in drought stress response and tolerance.” J.

Exp Bot. 58, 221-227 (2007).*

Seki M., Umezawa T., Urano K., Shinozaki K.:

“Regulatory metabolic networks in drought stress

responses.” Curr.Opin. Plant Biol. 10, 296-302 (2007).*

Tran L-S. P., Nakashima K., Shinozaki K., Yamaguchi-

Shinozaki K.: “Plant gene networks in osmotic stress

response: from genes to regulatory networks.” Methods

in Enzymology, 428, 109-128 (2007).

Hirayama T., Shinozaki K.: “Perception and transduction

of abscisic acid signals: keys to the function of the

versatile plant hormone ABA.” Trends in Plant Sci. 12,

343-351 (2007).*

Shinozaki K.: “Acceleration of soybean genomics using

large collection of DNA markers for gene discovery.”

DNA Res., 14, 235 (2008).

6.

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12.

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― 180 ―

Oral Presentations

Umezawa T., Yoshida R., Maruyama K., Yamaguchi-

Shinozaki K. and Shinozaki K.: SRK2C, a SnRK2

protein kinase that improves drought tolerance by

controlling stress-responsive gene expression in

Arabidopsis thaliana, Keystone Symposia on Plant Cell

Signaling: in vivo and omics Approaches, Feb. 1-6, Santa

Fe (2005).

Nishimura T., Kitahata N., Ishida J., Satou M., Seki M.,

Asami T., Shinozaki K. and Hirayama T.: Molecular

genetic analysis of novel ABA hypersensitive mutant,

ahg2, Keystone Symposia on Plant Cell Signaling: in

vivo and omics Approaches, Feb. 1-6, Santa Fe (2005).

Ecker J.R., Chen H., Sundarase A., Borevitz J., Cheuk

R., Kim C.J., Lim J., Ecker J.J., Mockler T., Shinn P.,

Meyers C., Karnes M., Koesema E., Ansari Y., Choy

N., Yamada K., Dale J.M., Wu S.X., Sakano H., Yu G.,

Chang C.H., Pham P., Hsuan V.W., Quach H.L., Jiang

P.X., Lee J.M., Toriumi M., Chan M.M.H., Tang C.C.,

Onodera C.S., Demg J.M., Goldsmith A.D., Vaysberg M.,

Aallender E.K., Wong C., Yamamura Y., Yuan S., Banh J.,

Banno F., Palm C.J., Southwick A.M., Jones T., Nguyen

M., Karlin-Newmann G., Lam B., Miranda M., Gurjal

M., Hansen N.F., Bowser L., Wu T., Tripp M., Tamse R.,

Seki M., Sakurai T., Satou M., Akiyama K., Carninci P.,

Enju A., Hayashizaki Y., Iida K., Ishida J., Arakawa T.,

Kawai J., kamiya A., Nakajima M., Marusaka M., Chan

S., Zhang X., Shinozaki K., Jacobsen S., Davis R.W. and

Theologis A.: Genome-wide discovery of transcription

units and functional elements in Arabidopsis, Keystone

Symposia on Plant Cell Signaling: in vivo and omics

Approaches, Feb. 1-6, Santa Fe (2005).

Ito T., Ohme-Takagi M. and Shinozaki K.: The MS1 gene

of Arabidopsis regulates pollen wall development, 16th

International Conference on Arabidopsis Research, Jun.

15-19, Wisconsin USA (2005)

Motohashi R., Satou M., Myouga F., Ohta D., Okikawa

A. and Shinozaki K.: Metabolomic analysis for

understanding of disrupted nuclear-encoded chloroplast

1.

2.

3.

4.

5.

【International Conferences】protein genes by FT-ICRMS in Arabidopsis thaliana,

16th International Conference on Arabidopsis Research,

Jun. 15-19, Wisconsin USA (2005)

Myouga F., Motohashi R., Iizumi H., Akiyama K. and

Shinozaki K.: Functional genomics of nuclear-encoded

chloroplast proteins in Arabidopsis, 16th International

Conference on Arabidopsis Research, Jun. 15-19,

Wisconsin USA (2005)

Seki M., Ishida J., Iida K., Nakajima M., Enju A., Sakurai

T., Kamei A., Oono Y., Umezawa T., Fujita M., Mizukado

S., Morosawa T., Akiyama K., Narusaka Y., Narusaka

M., Go M., Kobayashi M., Kawai J., Hayashizaki Y. and

Shinozaki K.: Expression profiling using Arabidopsis

whole-genome regulatory gene oligo DNA microarray

and production of Arabidopsis DNAbook containing

about 1000 RAFL cDNA for transcription factors, 16th



Matsui A., Yokoyama R., Seki M., Shinozaki K.,

Takahashi T., Komeda Y. and Nishitani K.: Functional

diversity and redundancy among members of the XTH

gene family: A specific role of AtXTH27 in vascular

development in rosette leaves of Arabidopsis, 16th



Kim J.M., Kuromori T., To T., Hirayama T., Seki M.

and Shinozaki K.: Functional analysis of AtORC1a,

Arabidopsis thaliana origin recognition complex, 16th



Kikuchi J. and Hirayama T.: Novel methods for uniform

stable isotope labeling in plant and animal systems

followed with a hetero-nuclear NMR-based metabomics,

Metabolomics 2005, Jun. 20-23, Tsuruoka Japan (2005)

Chikayama E., Saito K., Shinozaki K. and Kikuchi J.: A

novel method for metabolic profiling of hetero-nuclear

6.

7.

8.

9.

10.

11.


― 181 ―

NMR spectra based on pattern recognition algorithm,


Sekiyama Y., Saito K., Shinozaki K. and Kikuchi J.:

Preliminary investigation of methodological advances

in a hetero-nuclear NMR-based metabolomics,


Nakashima K., Tran L-S Phan., Fujita M., Maruyama

K., Todaka D., Ito Y., Shinozaki K. and Yamaguchi-

Shinozaki K.: Expression and functional analysis of

stress-inducible NAC transcription factors in rice plants,

Plant Biology 2005, Jul. 16-20, Seattle USA (2005)

Shinozaki K.: Plant gene networks in osmotic stress

response, 2005 Gordon Research Conference on Cellular

Osmoregulation: Sensors, Transducers and Regulators,

Aug 7-12, Newport USA (2005)

Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory

network of gene expression in abiotic stress response and

tolerance, 10th International Congress of SABRAO, Aug.

22-23, Tsukuba Japan (2005)

Nakashima K., Tran L-S Phan., Fujita M., Maruyama

K., Todaka D., Ito Y., Shinozaki K. and Yamaguchi-

Shinozaki K.: Molecular characterization of abiotic

stress-inducible OsNAC6, a member of the rice NAC

domain superfamily, 10th International Congress of

SABRAO, Aug. 22-23, Tsukuba Japan (2005)


M.M., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki

K. and Yamaguchi-Shinozaki K.: AREB1, an ABA-

induced transcription factor, plays a key role in drought

stress response in Arabidopsis thaliana, 10th International

Congress of SABRAO, Aug. 22-23, Tsukuba Japan

(2005)

Ito T., Ito Y., Maruyama K., Shinozaki K, and Yamaguchi-

Shinozaki K.: Functional analysis of rice transcription

factor OsDREB1F involved in environmental stress-

inducible gene expression, 10th International Congress

of SABRAO, Aug. 22-23, Tsukuba Japan (2005)

Shinozaki K., Yamaguchi-Shinozaki K., Seki M.,

Osakabe Y., Yoshida R., Maruyama K. and Umezawa

T.: Gene networks involved in drought and cold stress

responses, Plant GEMs Amsterdam 2005 on Plant

Genomics European Meetings, Sep 20-23, Amsterdam

The Netherlands (2005)


Kuroda H., Kondou Y., Tsuhara Y., Suzuki K., Ishikawa

A., Seki M., Fujita M., Motohashi R., Nagata N., Takagi

T., Shinozaki K. and Matsui M.: Characterization of two

dominant mutants caused by ectopic expression of full-

length cDNA in Arabidopsis FOX hunting system, Plant

GEMs Amsterdam 2005 on Plant Genomics European

Meetings, Sep 20-23, Amsterdam The Netherlands (2005)

Shinozaki K. and Yamaguchi-Shinozaki K.: Gene

networks involved in drought stress responses and

tolerance, The 2nd International Conference on Integrated

Approaches to Sustain and Improve Plant Production

Under Drought Stress, Sep. 24-28, Rome Italy (2005)

Seki M., Ishida J., Nakajima M., Enju A., Sakurai T., Iida

K., Satou M., Akiyama K., Oono Y., Fujita M., Mizukado

S., Kamei A., Narusaka M., Narusaka Y., Morosawa T.,

Kim J.M., Yamaguchi-Shinozaki K., Go M., Toyoda T.

and Shinozaki K.: Arabidopsis encyclopedia using full-

length cDNAs and its application for expression profiling

under abiotic stress conditions, The 2nd International

Conference on Integrated Approaches to Sustain and

Improve Plant Production Under Drought Stress, Sep.

24-28, Rome Italy (2005)

Urano K., Maruyama K., Takeda M., Suzuki H., Saito

K., Shibata D., Yamaguchi-Shinozaki K. and Shinozaki

K.: Metabolome and transcriptome analysis of ABA

responsive pathway under drought stress in Arabidopsis

thaliana, The 2nd International Conference on Integrated

Approaches to Sustain and Improve Plant Production

Under Drought Stress, Sep. 24-28, Rome Italy (2005)

Ito Y., Katsura K., Taji T., Kobayashi M., Seki

M., Shinozaki K. and Yamaguchi-Shinozaki K.:

12.

13.

14.

15.

16.

17.

18.

19.

20.

21.

22.

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24.


― 182 ―

Overexpression of the DREB1/CBF gene family

improved stress tolerance to drought, high salt and low

temperature in rice, The 2nd International Conference

on Integrated Approaches to Sustain and Improve Plant

Production Under Drought Stress, Sep. 24-28, Rome

Italy (2005)

Umezawa T.: Arabidopsis SNRK2 protein kinases and

osmotic stress signaling, Xth France-Japan Workshop on

Plant Sciences, Sep. 25-29, Toulouse France (2005)

Yamaguchi-Shinozaki K., Osakabe Y., Mizuno S.,

Maruyama K., Seki M. and Shinozaki K.: A leucine-

rich repeat receptor like kinase involved in perception of

ABA signal, Protein Phosphorylation in plant Signaling

2005, Oct. 20-21, Tsukuba Japan (2005)

Yoshida R., Umezawa T., Ichimura K., Mizoguchi T.,

Takahashi F., Takahashi S. and Shinozaki K.: Arabidopsis

SnRK2 plays important roles in both ABA and osmotic

stress signaling, Protein Phosphorylation in plant

Signaling 2005, Oct. 20-21, Tsukuba Japan (2005)

Seki M., Ishida J., Nakajima M., Enju A., Sawasaki

T., Endo Y., Oono Y., Morosawa T., Kim J.M., Sakurai

T., Iida K., Kamei A., Narusaka M., Narusaka Y. and

Shinozaki K.: Arabidopsis functional genomics of protein

kinases and transcription factors, Protein Phosphorylation

in plant Signaling 2005, Oct. 20-21, Tsukuba Japan

(2005)

Mizuno S., Osakabe Y., Maruyama K., Shinozaki K.

and Yamaguchi-Shinozaki K.: Regulation of floral

development by the LRR receptor protein kinase

2(RPK) in Arabidopsis, Protein Phosphorylation in plant



M.M., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki

K. and Yamaguchi-Shinozaki K.: AREB1 regulates novel

ABRE-dependent ABA-signaling that enhances drought

stress tolerance during the vegetative growth phase in

Arabidopsis thaliana, Protein Phosphorylation in plant


Takahashi F., Yoshida R., Ichimura K., Mizoguchi T.,

Yonezawa M., Maruyama K., Yamaguchi-Shinozaki K.

and Shinozaki K.: A novel MAP kinase cascade, MKK3-

MPK6, regulates jasmonate signaling in Arabidopsis,

Protein Phosphorylation in plant Signaling 2005, Oct.

20-21, Tsukuba Japan (2005)

Osakabe Y., Maruyama K., Seki M., Shinozaki K.

and Yamaguchi-Shinozaki K.: An LRR receptor

kinase, RPK1, is a key membrane-bound regulator of

abscisic acid early signaling in Arabidopsis, Protein

Phosphorylation in plant Signaling 2005, Oct. 20-21,

Tsukuba Japan (2005)

Yoshida T., Nishimura N., Kitahata N., Kuromori

T., Ito T., Asami T., Shinozaki K. and Hirayama T.:

ABA Hypersensitive Germination3 encodes a protein

phosphatase 2C(AtPP2CA) that strongly ABA signaling

during germination among Arabidopsis PP2Cs, Protein

Phosphorylation in plant Signaling 2005, Oct. 20-21,


Umezawa T., Yoshida R., Maruyama K., Yamaguchi-

Shinozaki K. and Shinozaki K.: An osmotic-stress

activated protein kinase SRK2C mediates stress signaling

in Arabidopsis, Protein Phosphorylation in plant


Katagiri T., Kobayashi M. and Shinozaki K.: An

important role of phosphatidic acid in ABA signaling

during germination in Arabidopsis thaliana, International

Conference on Plant Lipid-Mediated Signaling: Building

Connections, Oct. 26-29, Raleigh USA (2005)

Yamaguchi-Shinozaki K. and Shinozaki K.: Functional

analysis of plant transcription factors involved in abiotic

stress response, CREST-JST International Symposium

2005 on Functional Network of Transcription Factors in

Plants, Nov. 16-17, Tsukuba Japan (2005)

Mitsuda N., Seki M., Shinozaki K. and Ohme-Takagi

M.: Transcription factors regulating secondary wall

thickenings, CREST-JST International Symposium 2005

25.

26.

27.

28.

29.

30.

31.

32.

33.

34.

35.

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― 183 ―

on Functional Network of Transcription Factors in Plants,

Nov. 16-17, Tsukuba Japan (2005)

Seki M., Ishida J., Nakajima M., Enju A., Morosawa

T., Kim J.M., Sakurai T., Iida K., Oono Y., Kamei A.,

Narusaka M., Narusaka Y., Toyoda T., Mochizuki Y.,

Hasegawa Y., Yamaguchi-Shinozaki K. and Shinozaki

K.: Arabidopsis whole-genome expression profiling

under abiotic stress conditions, CREST-JST International

Symposium 2005 on Functional Network of Transcription

Factors in Plants, Nov. 16-17, Tsukuba Japan (2005)

Fujita M., Fujita Y., Maruyama K., Seki M., Ohme-

Takagi M., Yamaguchi-Shinozaki K. and Shinozaki K.: A

dehydration-induced NAC protein, RD26 is involved in a

novel ABA-dependent stress-signaling pathway, CREST-

JST International Symposium 2005 on Functional

Network of Transcription Factors in Plants, Nov. 16-17,


Shinozaki K. and Yamaguchi-Shinozaki K.: Functional

genomics for gene discovery in abiotic stress response

and tolerance, 5th International Rice Genetics

Symposium and 3rd International Rice Functional

Genomics Symposium, Nov. 19-23, Manila Philippines

(2005)

Yamaguchi-Shinozaki K. and Shinozaki K.: Rice

promoters for the generation of stress tolerant transgenic

crops, 5th International Rice Genetics Symposium and

3rd International Rice Functional Genomics Symposium,

Nov. 19-23, Manila Philippines (2005)

Todaka D., Ito Y., Ohme-Takagi M., Shinozaki K.

and Yamaguchi-Shinozaki K.: Functional analysis of

OsPIF1 gene down-regulated by drought stress in rice,

5th International Rice Genetics Symposium and 3rd

International Rice Functional Genomics Symposium,

Nov. 19-23, Manila Philippines (2005)

38.

39.

40.

41.

42.

Shinzoaki K. and Yamaguchi-Shinozaki K.: Molecular

responses and tolerance to drought stress in higher plants,

The First International Conference on the Theory and

Plactices in Biological Water Saving, May 21-25, Beijing

43.

China (2006).

Shinozaki K. and Yamaguchi-Shinozaki K.: Gene

networks involved in drought stress responses and

tolerance, Bio Vision Alexandira 2006, Apri.26-29,

Alexandria Egypt (2006).

Shinozaki K. and Yamaguchi-Shinzoaki K.: Signaling

and transcription networks in drought stress response,

Keystone Symposia on Plant Responses to Abiotic Stress,

April 8-13, Colorado USA (2006).

Seki M., Ishida J., Morosawa T., Matusi A., Kim

J.M., Nakajima M., Enju A., To T., Iida K., Go M.,

Mochizuki Y., Hasegawa Y., Toyoda T. and Shinzoaki

K.: “Arabidopsis whole-genome transcriptome analysis

under abiotic stress conditions”, The 16th Penn State

Symposium in Plant Physiologyon RNA Biology: Novel

Insights from Plant Systems, May 18-20, Penn State

USA (2006).

Ito T., Nagata N., Ohme-Takagi M., Ma H. and

Shinozaki K.: An Arabidopsis MALE STERILITY1 gene

encoding a PHD-type transcription factor regulates early

microgametogenesis, The 16th Penn State Symposium in

Plant Physiology on RNA Biology: Novel Insights from

Plant Systems, May 18-20, Penn State USA (2006).

Hirayama T., Tian C., Tsuboi Y., Chikayama E.,

Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:

Advances in hetero-nuclear NMR-based metabolomics

approach in plant systems, 20th IUBMB Internationa

Congress of Biochemistry and Molecular Biology and

11th FAOBMB Congress, June 18-23, Kyoto Japan

(2006).

Tsuboi Y., Hirayama T., Shinozaki K. and Kikuchi J.:

Toward an NMR-based nutrigenomics assisted by stable

isotope labeling of plants through animals, 20th IUBMB

Internationa Congress of Biochemistry and Molecular

Biology and 11th FAOBMB Congress, June 18-23,

Kyoto Japan (2006).

Hirayama T., Tsuboi Y., Shinozaki K. and Kikuchi

44.

45.

46.

47.

48.

49.

50.


― 184 ―

J.: Toward an NMR-based nutrigenomics assisted

by stable isotope labeling of plants through animals,

Metabolomics 2006 on the Second Scientific Meeting

of the Metabolomics Society, June 24-29, Boston USA

(2006).

Tian C., Tsuboi Y., Chikayama E., Sekiyama Y.,

Kuromori T., Shinozaki K., Hirayama T. and Kikuchi J.:

An efficient strategy for the metabolic characterization of

Arabidopsis mutants with visible and silent phenotypes

by fast 1D-NMRscreening followed multi-dimensional

NMR analysis, Metabolomics 2006 on the Second

Scientific Meeting of the Metabolomics Society, June

24-29, Boston USA (2006).

Ichikawa T., Nakazawa M., Kondou Y., Ishikawa A.,

Kawashima M., Iizumi H., Seki M., Fujita M., Muto S.,

Shinozaki K. and Matsui M.: Evaluation of Arabidopsis

FOX line: T2 phenotype and phenotype recapitulation

by transformation, 17th International Conference on

Arabidopsis Research, June 28- July 2, Wisconsin-

Madison USA (2006).

Ichimura K., Casais C., Peck S., Shinozaki K. and

Shirasu K.: MEKK1 negatively regulates tissue specific

and temperature dependent cell death in Arabidopsis,

17th International Conference on Arabidopsis Research,

June 28- July 2, Wisconsin-Madison USA (2006).

Katagiri T. and Shinozaki K.: Functional analysis of

phosphatidic acid during germination, 17th International

Conference on Arabidopsis Research, June 28- July 2,

Wisconsin-Madison USA (2006).

Motohashi R., Okada E., Kuroda H., Nagata N., Matsui

M. and Shinozaki K.: Functional analysis of ribosome

binding factor (RBFA) homologue using Ac/Ds

transposon system in Arabidopsis thaliana. Plant Biology

2006, Aug. 5-9, Boston USA (2006).


Shinozaki K. and Hirayama T.: Characterization of novel

ABA hypersensitive mutant, ahg1,Plant Biology 2006,

Aug. 5-9, Boston USA (2006).

Hirayama T., Tian C., Tsuboi Y., Chikayama E.,

Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:

Adcances in hetero-nuclear NMR-based metabolomics

approach in plant systems, Plant Biology 2006, Aug. 5-9,

Boston USA (2006).

Hayashi S., Kuromoti T., Shinozaki K. and Hirayama T.:

Analysis of an Arabidopsis root hair defective mutant

shv3, Plant Biology 2006, Aug. 5-9, Boston USA (2006).

Yoshida T., Nishimura N., Kitahata N., Kuromori

T.,Kuroda H., Matsui M., Asami T., Shinozaki K. and

Hirayama T.: ABA-hypersensitive germination3 encodes

a protein phosphatase 2C (AtPP2CA) that strongly

regulates abscisic acid signaling during germination

among Arabidopsis protein phosphatase 2Cs, Plant

Biology 2006, Aug. 5-9, Boston USA (2006).

Shinozaki K. and Yamaguchi-Shinozaki K.: Regulatory

gene networks in drought stress responses and tolerance,

Tropical Crop Biotechnology Conference 2006, Aug.

16-19, Cairns Queensland Australia (2006).

Shinzoaki K. and Yamaguchi-Shinozaki K.: Regulatory

networks in drought stress response and tolerance, 8th

International Congress of Plant Molecular Biology, Aug.

20-25, Adelaide Australia (2006).

Seki M., Ishida J., Morosawa T., Matsui A., Kim J.M.,

Nakajima M., Enju A., To T., Iida K., Go M., Mochizuki

Y., Hasegawa Y., Kaminuma E., Toyoda T. and Shinozaki

K.: Arabidopsis whole-genome expression profiling under

abiotic stress conditions, 8th International Congress of

Plant Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

Myouga F., Akiyama K., Sakurai T., Motohashi R. and

Shinozaki K.: Gene discovery for chloroplast proteins

involved in drought tolerance in Arabidopsis using

the rarge database, 8th International Congress of Plant

Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

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― 185 ―

Yasuda M., Ishikawa A., Asami T., Kamiya Y., Shinozaki

K., Kudo T., Yoshida S. and Nakashita H.: Antagonistic

interaction between environmental stress response

and systemic acquired resistance in Arabidopsis, 8th



Kim J.M., Kuromori T., To T., Hirayama T., Seki M.

and Shinozaki K.: Functional analysis of AtORC1a in a

phase progression, 8th International Congress of Plant

Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

Nakamura H., Hakata M., Kajikawa M., Ando S.,

Amano K., Toki N., Higashi N., Fujita M., Enju A., Seki

M., Nakazawa M., Ichikawa T., Matsui M., Shinzoaki

K., Miyao A., Toki T., Hirochika H. and Ichikawa H.:

Genome-wide functional analysis of rice genes using

FOX hunting system 1. Overview and current status, 8th



Hakata M., Nakamura H., Asano T., Kajikawa M.,

Amano K., Toki N., Higashi N., Fujita M., Enju A.,

Seki M., Nakazawa M., Ichikawa T., Shinozaki K.,

Matsui M., Komatsu S., Miyao A., Toki S., Hirochika

H. and Ichikawa H.: Genome-wide functional analysis

of rice genes using FOX hunting system 2. Analysis and

identificaiton of introduced cDNAs in the FOX-rice lines,

8th International Congress of Plant Molecular Biology,

Aug. 20-25, Adelaide Australia (2006).

Ichikawa H., Nakamura H., Hakata M., Kajikawa M.,

Ando S., Amano S., Toki N., Higashi N., Fujita M.,

Enju A., Seki M., Nakazawa M., Ichikawa T., Matsui

M., Shinozaki K., Miyao A., Toki T. and Hirochika H.:

FOX hunting system: a genome-wide functional analysis

of rice genes using transgenic plants, 8th International

Congress of Plant Molecular Biology WorkShop, Aug.


Urano K., Maruyama K., Takeda M., Suzuki H., Saito

K., Shibata D., Yamaguchi-Shinozaki K. and Shinozaki

K.: Analysis of ABA-dependent metabolic networks

in drought stress response, 2006 Gordon Research

Conference on Salt & Water Stress in Plants, Sep. 3-8,

Oxford UK (2006).

Tran L-S., Urao T., Qin F., Osakabe Y., Kakimoto T.,


analyses of AtHK1, AHK2, AHK3 and AHK4/CRE1

in response to abiotic stresses, 2006 Gordon Research

Conference on Salt & Water Stress in Plants, Sep. 3-8,

Oxford UK (2006).

Fujita Y., Fujita M., Satoh R., Maruyama K., Sayama

H., Seki M., Hiratsu K., Ohme-Takagi M., Shinzoaki K.

and Yamaguchi-Shinozaki K.: AREB1 mediates novel

ABRE-dependent ABA-signaling that enhances drought

stress tolerance in Arabidopsis thaliana, 2006 Gordon

Research Conference on Salt & Water Stress in Plants,

Sep. 3-8, Oxford UK (2006).

Shinozaki K.: Functional genomics in RIKEN Plant

Science Center for the improvement of plant productivity,

PKU-CCAST-RIKEN Joint Symposium-Life Science

Panel, Nov. 10, Beijing China (2006).


networks of gene expression in environmental stress

response, GARNet 2006, Sep 11-12, Bristol UK (2006).

Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,

Maruyama K., Todaka D., Ito Y., Shinozaki K. and

Yamaguchi-Shinozaki K.: Functional analysis of NAC-

type transcription factor OsNAC6 involved in abiotic

stress-responsive gene expression in rice, 4th Internationa

Rice Functional Genomics Symposium, Oct. 9-11,

Montpellier France (2006).

Umezawa T., Todaka D., Totoki Y., Sakurai T., Seki M.,

Toyoda A., Maruyama K., Nakashima K., Mizukado S.,

Kasuga M., Ahmed S, Sado H., Harada K., Tsubokura Y.,

Sato S., Anai T., Ishimoto M., Funatsuki H., Teraishi M.,

Shinano T., Akashi R., Sakaki Y., Yamaguchi-Shinozaki

K. and Shinozaki K.: Construction of a soybean full-

length cDNA library and oligonucleotide microarray, The

KAZUSA Conference on legume Genetics and Genomics

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― 186 ―

in Asia, Nov. 27-28, Kisarazu Japan (2006).

Todaka D., Nakashima K., Ito Y., Ohme-Takagi M.,


analysis of the OsPIF1 gene down-regulated by drought

stress in rice. ICGEB Meeting: Biotic and Abiotic Stress

Responses in Plants, Dec 11-13, New Delhi, India. (2006)

.

Qin F., Sakuma Y., Tran L-S P., Osakabe Y., Shinozaki

K. and Yamaguchi-Shinzoaki K.: The function and

regulation of an Arabidopsis transcription factor

DREB2A in plant water deficit and high temperature

stresses, Gordon Research Conferences on Temperature

Stress in Plants, Jan 21-26, Ventura CA (2007).

Osakabe Y., Mizuno S, Shinozaki K. and Yamaguchi-

Shinozaki K.: Functional analysis of a leucine-rich

repeat receptor like kinase, RPK1, involved in ABA

signal transduction of Arabidopsis, Gordon Research

Conferences on Temperature Stress in Plants, Jan 21-26,

Ventura CA (2007).

Ito Y., Shinozaki K. and Yamaguchi-Shinozaki K.:

Comprehensive analysis of 10 DREB/CBF family genes

in rice, Gordon Research Conferences on Temperature

Stress in Plants, Jan 21-26, Ventura CA (2007).

Kidokoro S., Maruyama K., Nakashima K., Sakuma

Y., Imura Y., Shinozaki K. and Yamaguchi-Shinozaki

K.: Transcriptional regulation of the DREB1C gene

in response to low temperature, Gordon Research

Conferences on Temperature Stress in Plants, Jan 21-26,

Ventura CA (2007).

Seki M., Matsui A., Kim J-M., Ishida J., Nakajima

M., Morosawa T., Kawashima M., Satou M., Kim T.,

Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,

Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis

whole-genome transcriptome analysis under drought,

cold, high-salinity, and ABA treatment conditions

using tiling array and 454 sequencing technology, 24th

Symposium in Plant Biology on Gene Silincing: The

Biology of Small RNAs and the Epigenome, Jan. 18-20,

Riverside USA (2007).

Shinozaki K.: Functional genomics in drought stress

responses, CINA-JAPAN Joint Workshop on Plant

Molecular Breeding, Mar 12-13, Beijing China (2007).

Shinozaki K.: Molecular biology and salinity stress

responses and tolerance, BIOTEC-RIKEN Research

Forum, May 22-25, Thailand (2007).

Shinozaki K., Yamaguchi-Shinozaki K. and Seki M.:

Regulatory gene network in drought and ABA responses.,

18th International Conference on Arabidopsis Research.,

June 20-23., Beijing China (2007).

Mitsuda N., Iwase A., Yamamoto H., Yoshida M.,

Seki M., Shinozaki K. and Ohme-Takagi M.: NAC

transcription factors NST1, NST2 and NST3 are key

regulators of secondary wall formation in Arabidopsis,

18th Internat ional Conference on Arabidopsis

Research.,June 20-23., Beijing China (2007).


Kuroda H., Kondou Y., Ishikawa A., Seki M., Fujita

M., Higuchi M., Shinozaki K. and Matsui M.: The Fox

hunting system: an alternative gain-of- function gene

hunting technique, 18th International Conference on

Arabidopsis Research.,June 20-23., Beijing China (2007).

Seki M., Matsui A., Kim J-M., Ishida J., Nakajima

M., Morosawa T., Kawashima M., Satou M., Kim T.,

Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,

Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis

whole-genome transcriptome analysis under drought,

conditions using tiling array and 454 sequencing

technology, 18th International Conference on Arabidopsis

Research.,June 20-23., Beijing China (2007).

Kuromori T., Kamiya A., Ito T., Wada T., Hirayama T.

and Shinozaki K.: A mutant-based phenome analysis

and collection of mutant phenotype information, 18th

International Conference on Arabidopsis Research.,June

20-23., Beijing China (2007).

Myouga F., Iizumi H., Kuromori T., Motohashi R. and

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― 187 ―

Shinozaki K.: Characterization of Ds-tagged mutants for

nuclear-encoded chloroplast protein involved in plastid

development and oxi-dative stress responses, 18th



Hirayama T., Narusaka Y., Yasuda M., Narusaka M.,

Hayashi S., Kitahata N., Kuromori T., Asami T. and

Shinozaki K.: Impairment in the mRNA degradation

causes abnormal response to both abscisic acid and

salicylic acid in Arabidopsis, 18th International

Conference on Arabidopsis Research.,June 20-23.,

Beijing China (2007).

Urano K., Maruyama K., Takeda M., Sakurai N., Suzuki

H., Saito K., Shibata D., Yamaguchi-Shinozaki K. and

Shinozaki K.: Comprehensive analysis of ABA-regulated

metabolome and transcriptome in drought response

of Arabidopsis, 18th International Conference on

Arabidopsis Research.,June 20-23., Beijing China (2007).

Takahashi F., Yoshida R., Ichimura K., Mizoguchi T.,

Yonezawa M., Maruyama K., Yamaguchi-Shinozaki K.

and Shinozaki K.: The MAP kinase cascade MKK3-

MPK6 is an important part of the jasmonate signal

transduction pathway in Arabidopsis, 18th International

Conference on Arabidopsis Research.,June 20-23.,

Beijing China (2007).

Garcia M., Myouga F., Takechi K., Sato H., Nabeshima

K., Nagata N., Takio S., Shinozaki K. and Takano H.:

An Arabidopsis chloroplast-targeting MurE homolog

has an essential role in chloroplast development, 18th




gene networks in drought and ABA responses, IPGSA

19th Annual Meeting, July21-25, Puerto Vallarta Mexico

(2007).

Ayano M., Godal H., Shinozaki K., Watanabe N.,

Yokoyama M. and Yoshida S.: An oxylipin, (12Z, 15Z)-

9-hydroxy-10-oxo octadecadienoic acid [KODA] shows

unique signaling on floral development of higher plants,

IPGSA 19th Annual Meeting, July21-25, Puerto Vallarta

Mexico (2007).

Ayano M., Godal H., Shinozaki K., Watanabe N.,

Yokoyama M. and Yoshida S.: Function analysis of

oxylipin, 9-hydroxy-10-oxo12(z), 15(z)-octadecadienoic

acid (KODA) in Arabidopsis thaliana, IPGSA 19th

Annual Meeting, July21-25, Puerto Vallarta Mexico

(2007).


Shinozaki K. and Hirayama T.: ABA-hypersensitive

germination encodes a protein phosphatase 2C, an

essential component of abscisic acid signaling in

Arabidopsis seed, IPGSA 19th Annual Meeting,

July21-25, Puerto Vallarta Mexico (2007).

Goda H., Yoshida S., Shinozaki K. and Shimada Y.:

Finding of a novel hormone-related compound by using

AtGenExpress microarray data, IPGSA 19th Annual

Meeting, July21-25, Puerto Vallarta Mexico (2007).


gene network in drought stress response, 2nd World

Conference of Stress, August 23-26, Butapest Hungary

(2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Improving

abiotic stress-tolerance in crops,Frontiers in Transgenesis,

Sep 26-28, St Louis USA (2007).


networks of gene expression in abiotic stress responses in

Arabidopsis and rice, 2007 International Symposium and

Annual Meeting of the KSABC, Oct 11-13, Gyeongiu

Korea, (2007).

Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama

K., Shinozaki K. and Yamaguchi-Shinozaki K.:

Functional analysis of the OsDREB2B gene encoding

a rice transcription factor involved in abiotic-stress-

responsive gene expression, 2007 International

Symposium and Annual Meeting of the KSABC, Oct

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― 188 ―

11-13, Gyeongiu Korea, (2007).

Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,


K. and Yamaguchi-Shinozaki K.: Functional analysis

of NAC-type transcription factor OsNAC6 involved in

abiotic stress-responsive gene expression in rice, 2007

International Symposium and Annual Meeting of the

KSABC, Oct 11-13, Gyeongiu Korea, (2007).

Yamada K., Osakabe Y., Maruyama K., Fujita Y., Mizoi J.,


analysis of osmotic stress-inducible sugar transporter

gene in Arabidopsis. 2007 International Symposium and


Korea, (2007).

Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-

Shinozaki K.: Comprehensive analysis of DREB1/CBF

family genes in rice, 2007 International Symposium and


Korea, (2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Improving

transgenic tolerance to abiotic stresses including

drought in rice, The 5th International Symposium of rice

functional genomics,Oct. 15-17, Tsukuba Japan (2007).



analysis of the OsPIF1 gene down-regulated by drought

stress in rice.The 5th International Symposium of rice

functional genomics, Oct. 15-17, Tsukuba Japan (2007).

Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-

Shinozaki K.: Comprehensive analysis of DREB1/CBF

family genes in rice, The 5th International Symposium

of rice functional genomics,Oct. 15-17, Tsukuba Japan

(2007).

Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama

K., Shinozaki K. and Yamaguchi-Shinozaki K.:

Functional analysis of the OsDREB2B gene encoding

a rice transcription factor involved in abiotic-stress-

responsive gene expression,The 5th International

Symposium of rice functional genomics, Oct. 15-17,

Tsukuba Japan (2007).

Nakashima K., Tran L-S Phan., Nguyen D.V., Fujita M.,


K. and Yamaguchi-Shinozaki K.: Functional analysis of

a NAC-type transcription factor OsNAC6 involved in

abiotic and biotic stress-responsive gene expression in

rice, The 5th International Symposium of rice functional

genomics, Oct. 15-17, Tsukuba Japan (2007).


Gene network in drought stress response, 2007 Genomics

Symposium on The Genomics of Drought, Oct 22-24,

Adelaide Australia (2007).

Shinozaki K.: Plant functional genomics in drought stress

response, National Tipei University Special Seminar, Oct

15-18, Taipei China (2007).

Seki M.: Arabidopsis whole-genome transcriptome

analysis under drought, cold, high-salinity, and

ABA treatment conditions using tiling array and 454

sequencing technology, 2007 Iwate International

Symposium on Biological Sciences --The Impacts of

OMICS--, Oct. 29, Morioka Japan (2007).


Plant functional genomics in drought stress response,

The 6th Asian Crop Science Association Conference and

The 2nd International Conference on Rice for the Future,

Nov. 5-9, Bangkok Thailand (2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Regulation

of gene expression in response to abiotic stresses and

improving stress tolerance in crops, Agricultural Plant

Stress Response VII on abiotic stress and Plant Growth

and Development, Nov. 8, Gwangju Korea (2007).

Arinaga N., Osakabe Y., Yamada K., Maruyama K.,


analysis of an osmotic stress-inducible potassium

transporter, KUP6, in Arabidopsis, Agricultural Plant

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― 189 ―

Stress Response VII on abiotic stress and Plant Growth

and Development, Nov. 8, Gwangju Korea (2007).

Maruyama K., Takeda M., Sakurai N., Kidokoro S.,

Yamada K., Suzuki H., Saito K., Shinozaki K. and

Yamaguchi-Shinozaki K.: A comparative analytic

approach to the chilling/drought metabolites and

transcripts: DREB1A/CBF3 and chloroplastic raffinose

synthase playing a key role in low-temperature condition,

Agricultural Plant Stress Response VII on abiotic stress

and Plant Growth and Development, Nov. 8, Gwangju

Korea (2007).

Kanamori N., Fujita Y., Yoshida T., Sayama H., Fujita

M., Maruyama K., Hiratsu K., Ohme-Takagi M.,

Seki M., Shinozaki K. and Yamaguchi-Shinozaki K.:

Molecular characterization of AREB family transcription

factors involved in ABA-responsive gene expression in

Arabidopsis thaliana, Agricultural Plant Stress Response

VII on abiotic stress and Plant Growth and Development,

Nov. 8, Gwangju Korea (2007).

Osakabe Y., Mizuno S., Maruyama K., Osakabe K.,


analysis of a leucine-rich repeat receptor like kinase,

RPK1, involved in ABA signal transduction of

Arabidopsis, Agricultural Plant Stress Response VII on

abiotic stress and Plant Growth and Development, Nov. 8,

Gwangju Korea (2007).



analysis of the OsPIFI gene down-regulated by drought

stress in rice, Agricultural Plant Stress Response VII on

abiotic stress and Plant Growth and Development, Nov. 8,

Gwangju Korea (2007).

Yoshida T., Sakuma Y., Todaka D., Osakabe Y.,

Maruyama K., Shinozaki K. and Yamaguchi-Shinozaki

K.: Functional analysis of Arabidopsis HsfA3 controlled

by DREB2A regulatory system under heat stress,

Agricultural Plant Stress Response VII on abiotic stress

and Plant Growth and Development, Nov. 8, Gwangju

Korea (2007).

Fujita Y., Yamaguchi-Shinozaki K. and Shinozak K.:

Improving drought and salt stress tolerance in plants

by gene transfer, Fourth Biomass-Asia Workshop, Nov

20-22, Selangor Darul Ehsan, Malaysia (2007).

Tanaka R., Nagane R., Myouga F., Motohashi R.,

Shinozaki K. and Tanaka A.: HPLC-based screening for

pigment metabolism mutants in Arabidopsis thaliana, 7th

International Conference on Tetrapyrrole Photoreceptors

in Photosynthetic Organisms, Dec. 9-14, Kyoto Japan

(2007).

Shinozaki K., Urano K., Maruyama K., Takeda M.,

Ogata Y., Sakurai N., Suzuki H., Saito K., Shibata D.,

Kobayashi M. and Yamaguchi-Shinozaki K.: Regulatory

gene network in drought-induced metabolic change,

5th International Conference on Plant Metabolomics,

Yokohama Japan, Jul 15-18 (2008).

Mochida K., Furuta T., Ebana K., Ogihara Y., Shinozaki

K and Kikuchi J: Metabolic phenotyping of genetic

resources in gramineae using NMR, 5th International

Conference on Plant Metabolomics, Yokohama Japan,

Jul 15-18 (2008).

Akiyama K., Chikayama E., Yuasa H., Shimada Y.,

Tohge T., Shinozaki K., Yokota-Hirai M., Sakurai T.,

Kikuchi J. and Saito K.: PRIME: The Platform for

RIKEN metabolomics to assist systems biology studies,

5th International Conference on Plant Metabolomics,


Sakurai T., Kondou Y., Akiyama K., Sakakibara H.,

Kusano M., Takahashi H., Mori M., Takatsuji H.,

Shinozaki K., Oda K., Hirochika H. and Matsui M.:

Construction of RIKEN rice FOX Arabidopsis mutant

line database, 5th International Conference on Plant

Metabolomics, Yokohama Japan, Jul 15-18 (2008).

Urano K., Maruyama K., Takeda M., Ogata Y., Sakurai

N., Suzuki H., Saito K., Shibata D., Kobayashi

M., Yamaguchi-Shinozaki K. and Shinozaki K.:

Characterization of the ABA-regulated global responses

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― 190 ―

to dehydration in Arabidopsis by metabolomics, 5th

International Conference on Plant Metabolomics,


Maruyama K., Kidokoro S., Takasaki H., Sakurai N.,

Suzuki H., Saito K., Shibata D., Shinozaki K. and

Yamaguchi-Shinozaki K.: A comparative analysis of

transcripts and metabolites in rice under chilling and

dehydration conditions, 5th International Conference on

Plant Metabolomics, Yokohama Japan, Jul 15-18 (2008).

129.

【Domestic Conferences】　Total 210

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