bioremediation. bioremediation ? biology + remediation = bioremediation biological organisms...

Download Bioremediation. Bioremediation ? Biology + Remediation = Bioremediation Biological organisms (bacteria, fungi, plant) Method used to clean the contaminated

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  • Bioremediation

  • Bioremediation ?Biology + Remediation = Bioremediation

    Biological organisms (bacteria, fungi, plant)

    Method used to clean the contaminated area

    High toxic to less toxic (or) non toxic

  • PrinciplesMicroorganisms - take pollutants from the environment - used to enhance the growth and metabolic activity

    Bacteria, Fungi are well known for degrading complex molecules and transform the product into part of their metabolism

  • DefinitionThe process whereby organic wastes are biologically degraded under controlled conditions to an innocuous state.

    Bioremediation is the use of living organisms, primarily microorganisms, to degrade the environmental contaminants into less toxic forms.

  • ProcessMicroorganisms release enzymes to breakdown the contaminant into digestible farm

  • BIOREMEDIATIONIn situEx situBioventingBiospargingBiostimulationBioaugmentationPhytoremediationLand farmingCompostBiopilesBioreactors

  • BioventingThe most common in situ treatment

    Supplying air and nutrients through wells to contaminated soil to stimulate the indigenous bacteria.

    Bioventing employs low air flow rates and provides only the oxygen necessary for the biodegradation while minimizing volatilization and release of contaminants to the atmosphere.

    It works for simple hydrocarbons and can be used where the contamination is deep under the surface.

  • Bioventing

  • BiospargingBiosparging involves the injection of air under pressure below the water table to increase groundwater oxygen concentrations and enhance the rate of biological degradation of contaminants by naturally occurring bacteria.

    Biosparging increases the mixing in the saturated zone and there by increases the contact between soil and groundwater.

    Low cost of installing small - diameter air injection points allows considerable flexibility in the design and construction of the system.

  • Biosparging

  • BiostimulationIt involves supplying oxygen and nutrients by circulating aqueous solutions through contaminated soils to stimulate naturally occurring bacteria to degrade organic contaminants.

    It can be used for soil and groundwater. Generally, this technique includes conditions such as the infiltration of water - containing nutrients and oxygen.

  • BioaugumentationBioremediation frequently involves the addition of microorganisms indigenous or exogenous to the contaminated sites.

    Two factors limit the use of added microbial cultures in a land treatment unit:

    1) nonindigenous cultures rarely compete well enough with an indigenous population to develop and sustain useful population levels and

    2) most soils with long-term exposure to biodegradable waste have indigenous microorganisms that are effective degrades if the land treatment unit is well managed.

  • Land formingIt is a simple technique in which contaminated soil is excavated and spread over a prepared bed and periodically tilled until pollutants are degraded.

    The goal is to stimulate indigenous biodegradative microorganisms and facilitate their aerobic degradation of contaminants.

    In general, the practice is limited to the treatment of superficial 1035 cm of soil.

    Since landfarming has the potential to reduce monitoring and maintenance costs, as well as clean-up liabilities, it has received much attention as a disposal alternative.

  • CompostingComposting is a technique that involves combining contaminated soil with nonhazardous organic amendants such as manure or agricultural wastes.

    The presence of these organic materials supports the development of a rich microbial population and elevated temperature characteristic of composting.

  • BiopilesBiopiles are a hybrid of landfarming and composting. Essentially, engineered cells are constructed as aerated composted piles.

    Typically used for treatment of surface contamination with petroleum hydrocarbons they are a refined version of landfarming that tend to control physical losses of the contaminants by leaching and volatilization.

    Biopiles provide a favorable environment for indigenous aerobic and anaerobic microorganisms.

  • BioreactorsSlurry reactors or aqueous reactors are used for ex situ treatment of contaminated soil and water pumped up from a contaminated plume.

    Bioremediation in reactors involves the processing of contaminated solid material (soil, sediment, sludge) or water through an engineered containment system.

    A slurry bioreactor may be defined as a containment vessel and apparatus used to create a three - phase (solid, liquid, and gas) mixing condition to increase the bioremediation rate of soil bound and water-soluble pollutants as a water slurry of the contaminated soil and biomass (usually indigenous microorganisms) capable of degrading target contaminants.

  • PhytoremediationPlants have been commonly used for the bioremediation process called Phytoremedation, which is to use plants to decontaminated soil and water by extracting heavy metals or contaminants.

    Plants that are grown in polluted soil are specialized for the process of Phytoremedation.

    The plants roots can extract the contaminant, heavy metals, by one of the two ways, either break the contaminant down in the soil or to suck the contaminant up, and store it in the stem and leaves of the plant.

    Usually the plant will be harvest and removed from the site and burned.

    Phytoremediation process is used to satisfy environmental regulation and costs less then other alternatives.

    This process is very affective in cleaning polluted soil.

  • TypesPhytoextractionPhytotransformationPhytostabilisationPhytodegradationRhizofiltration

  • PhytoextractionThe plants to accumulate contaminants into the roots and aboveground shoots or leaves.

    This technique saves tremendous remediation cost by accumulating low levels of contaminants from a widespread area.

    Unlike the degradation mechanisms, this process produces a mass of plants and contaminants (usually metals) that can be transported for disposal or recycling.

  • PhytotransformationRefers to the uptake of organic contaminants from soil, sediments, or water and, subsequently, their transformation to more stable, less toxic, or less mobile form.

    Metal chromium can be reduced from hexavalent to trivalent chromium, which is a less mobile and noncarcinogenic form.

  • PhytostabilizationThe plants reduce the mobility and migration of contaminated soil.

    Leachable constituents are adsorbed and bound into the plant structure.

    They form a stable mass of plant from which the contaminants will not reenter the environment.

  • Phytodegradation Breakdown of contaminants through the activity existing in the rhizosphere.

    This activity is due to the presence of proteins and enzymes produced by the plants or by soil organisms such as bacteria, yeast and fungi.

    Rhizodegradation is a symbiotic relationship that has evolved between plants and microbes.

    Plants provide nutrients necessary for the microbes to thrive, while microbes provide a healthier soil environment.

  • RhizofiltrationIt is a water remediation technique that involves the uptake of contaminants by plant roots.

    Rhizofiltration is used to reduce contamination in natural wetlands and estuary areas.

  • Limitations of BioremediationContaminant type & Concentration Environment Soil type condition & Proximity of ground waterNature of organism Cost benefit ratios : Cost Vs Env. Impact Does not apply to all surface Length of bioremediation process

  • Advantages Minimal exposure of on site workers to the contaminant

    Long term protection of public health

    The Cheapest of all methods of pollutant removal

    The process can be done on site with a minimum amount of space and equipment

    Eliminates the need to transport of hazardous material

    Uses natural process

    Transform pollutants instead of simply moving them from one media to another

    Perform the degradation in an acceptable time frame

  • DISADVANTAGESCost overrun

    Failure to meet targets

    Poor management

    Climate Issue

    Release of contaminants to environment

    Unable to estimate the length of time its going to take, it may vary from site.

  • Bacterial genera isolated from water and sediment samples of different lakes55 isolates

  • Screening of nitrate reducersNo reduction : -Less reduction : +Moderate reduction : ++High reduction : +++Nitrate reduction test Reduction of nitrate / nitrite to ammoniumAppearance of reddish orange colourBased on intensity of the colour

  • Potent isolates used for study (+++)Pseudomonas sp. (KW 1) Pseudomonas sp. (KW 8) Bacillus sp. (KS 1) Alcaligenes sp. (KS 3)Pseudomonas sp. (KS 5) Pseudomonas sp. (KS 7)Corynebacterium sp. (OW 1) Pseudomonas sp. (OW 6)Bacillus sp. (OW 8) Alcaligenes sp. (OS 1) Alcaligenes sp. (OS 6) Pseudomonas sp. (OS 9) Bacillus sp. (YW 1) Bacillus sp. (YW 4) Bacillus sp. (YW 7) Alcaligenes sp. (YS 5) and Bacillus sp. (YS 8). Out of 55 isolates 17 isolates was found to be potent in nitrate reduction

  • Nitrate reducing efficiency of bacteria in synthetic medium with 100 mg.L-1 of nitrate at 48 hrsGrowth - 95 x 103 cfu.mL-1 (KW1)NO3 - 80.2%, 78.9% (KW1, YW4)Nitrite - 0.75 mg.L-1 (YS8)Ammonium - 2.8 mg.L-1 (OS 1)

  • Based on the above results (> 70%) the following isolates were selected for further analysis of nitrate removal.

    A - Pseudomonas sp. (KW 1) B - Bacillus sp. (KS 1) C - Corynebacterium sp. (OW 1) D - Pseudomonas sp. (OW


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