biomedical agriculture: a new approach for developing crops for health henry j. thompson cancer...
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Biomedical Agriculture: A New Approach for Developing Crops for Health
Henry J. Thompson
Cancer Prevention Lab
Colorado State University
Fort Collins, CO 80523
To meaningfully reduce the physical, psychosocial, and economic consequences of chronic diseases such as obesity, diabetes, heart
disease and cancer through:
• Identification, development, and production of food crop varieties that show maximum human health benefit while retaining marketability in the global arena.
• Development of the tools and techniques require to understand how these food crops prevent chronic disease.
• Dissemination of knowledge to the global community to promote long-term healthy lifestyle changes.
Colorado State University Crops for HealthTM
A Transdisciplinary Research & Training Program inBiomedical Agriculture
PreventionTherapy
Chronic disease• Obesity
• Diabetes
• Heart disease
• Cancer
FoodSupplements
Bioactive food components
Nutrients
Potential disease-preventive mechanisms of vegetables and fruit
• Antioxidant activity (effector activity or marker?)• Antibacterial, antiviral, and anti-inflammatory
activity• Modulation of detoxification enzymes• Stimulation of the immune system• Decrease in platelet aggregation• Alteration in cholesterol metabolism• Modulation of steroid hormone concentrations and
hormone metabolism• Blood pressure reduction
Biomedical engagement
Overweight/Obesity
Diabetes-Type II
Cardiovascular Disease
Cancer
Glucose availability
Inflammation
Cellular oxidation
Plant Food-Health Conundrum
• The issue of botanical diversity.
• Not the right crop varieties (cultivars) in the supermarket Crops for Health
Epidemiological Evidence
• Strong inverse relationship between cancer incidence and VF intake– World Cancer Research Fund, American Institute for Cancer Research
(1997) Food, nutrition and the prevention of cancer: a global perspective. • Evidence strongest for colon cancer
– Concept challenged Schatzkin , New Engl. J. Med. 342:1149-55, 2000– For breast cancer, the relationship is moderately strong (WCRF:1997) – Smith-Warner: JAMA 285: 769-776, 2001; Riboli JAMA 2004 No
effect• Publications in 2002-2007 are mixed
– Reports of protection (lung and ovarian cancer) and lack of association (breast and bladder cancer)
– No reports of a positive association
Why the variability in study conclusions?
• No effect: variation and chance• Measurement errors: VF intake• There is a threshold that VF intake must
exceed for protection• Only certain VF matter: botanical family• VF can be protective
– but not all individuals benefit from increased VF
Food
• A collection of chemicals produced (synthesized) in a biological context– Propagation of that
plant species
• For people: food is simply a collection of chemicals that can be ingested
Food Chemical NomenclatureFoods Phytochemicals
(phytonutrients)
Matrix effects
Mammalian cells Gut microflora
Biological inert chemicals
Non-nutrient1000s
Individual chemicals
Bioactive Food ComponentsBAFC
Nutrients50
• Cannot be made• Required for normal cellular function• Absence results in disease
Targets
Drill Down Point: What are the nutrients
• Macronutrients– Protein (4)
• Essential amino acids• Non essentail amino acids
– Carbohydrate (4)• Available
– Mono,di, oligo– Starch
• Unavailable– Fiber
» Soluble» Insoluble
– Fat (9)• Essentail (omega-6 vs 3)• Nonessential
*Water
• Micronutrients– Vitamins
• Fat soluble• Water
soluble
– Minerals• Macro• Trace
elements
The Big Picture Vision
• ID America’s favorite 20 crops• Understand crop origins and breadth of each
crop’s genetic diversity• Select cultivars from each crop for evaluation• Identify the most cancer protective cultivars• Define a cultivar-based diet of menus and recipes• Evaluate in women at high risk for breast cancer
or its recurrence• Ultimate goal: a “certifiable disease prevention
diet”
JA Milner : NCI Road Map 2005
JA Milner : NCI Road Map 2005
CSU-Credential Cultivars
BOTANICAL FAMILIES COMPARISON
Actinidiacae
Agaricaceae Chenopodaceae
CompositeaeConvolvulac
CruciferaeCucurbitace
Ericaceae Gramineae
Leguminosae Liliaceae
Musaceae Rosaceae Rutaceae
SolanaceaeUmbellifera
Vitaceae
Bot
anic
al F
amil
ies
0 1 2 3Average Serving Per Day
Low FV
High FV
Diet
Nouveau Cuisine for Cancer Prevention
N= 118
RESEARCH QUESTION• When vegetable and fruit (VF) intake is
high what has a greater effect on levels of oxidation – focus on botanical families: powerful
phytochemicals [Five family diet]– diverse number of VF with many many
chemicals of varied activity [Seventeen family diet]
0.00 0.50 1.00 1.50 2.00 2.50
Actinidiaceae
Chenopodiaceae
Convolvulaceae
Cucurbitaceae
Graminae
Liliaceae
Rosaceae
Solanaceae
Vitaceae
n-a-day
17 family
5 family
DAILY SERVINGS OF VEGETABLES AND FRUIT FROM EACH BOTANICAL FAMILY
Cruciferae
Rutaceae
• 5 Family– Cruciferae– Chenopodiaceae– Liliaceae– Rutaceae– Solanaceae
• 17 Family– Chenopodiaceae
Ericaceae– Cruciferae
Graminae– Liliaceae
Leguminosae– Rutaceae
Musaceae– Solanaceae
Rosaceae– Actinidiaceae
Umbelliferae– Agaricaceae
Vitaceae– Compositae
Curcurbitaceae– Convolvulaceae
LESSONS LEARNED
• Diet is a powerful chemical tool box.
• You can cause rapid and at times dramatic shifts in the chemicals to which your cells are exposed.
• What you eat can make a difference in the body’s microenvironment.
Plant Food-Health Conundrum
• The issue of botanical diversity.
• Not the right crop varieties (cultivars) in the supermarket: Crops for Health
Dry Bean
Apple
Potato
Created Equal?
VisionProvide consumers with crop varieties in the
food market for which we have evidence of enhanced human health benefits
Experimental Overview
• Chemical Analysis– Antioxidants and phytochemicals
• Cell Culture– Cell accumulation
• In Vivo Screening Bioassay– Mammary carcinogenesis
– Colon carcinogenesis
Genetic Classification Centers of Domestication
Epiphany: what’s the most efficient approach by which to discover
microbe or pest resistance?...why not health traits?
IR64
IAC
165
M20
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Co 39 Patbyeo Gerdeh Dular Sadu-cho
Panicle/Seed Types in Current SNP set
Health and Supplements
• Nutrient supplements– Original concept: developed to prevent deficiency
diseases– Contemporary use: if some is good, more is better
• BAFC supplements– Drug concept: intervene in disease processes to
prevent disease
• Evidence of health benefit: limited• Evidence of harmful effects: growing
The Big Picture Vision
• ID America’s favorite 20 crops – (Crops of International Importance)
• Understand a crop’s origins and genetic diversity• Select cultivars from each crop for evaluation• Identify the most cancer protective cultivars• Define a cultivar-based diet of menus and recipes• Evaluate clinically• Ultimate goal:
– CSU: “certifiable disease prevention diet”
Colorado Cuisine Modular Diet
• Cultivar defined certification
Modular Unit (B,L,D)(credential certified)
• Macronutrient composition– Protein 20%kcal– Fat 30% (10/10/10)– Carbohydrate 50% kcal
• 15% kcal as fiber
• Breakfast: 300 kcal– 60 kcal Pr=15g– 90 kcal Fat=10g– 150 kcal CHO=37g
• Botanical Diversity– 18 Families– Each module:
• at least 3 botanical families• At least 0.5 servings per family
• The serving sizes: 1 medium piece of fruit, ½ cup fruit or vegetable (raw, cooked, canned or frozen), 1 cup leafy green salad, ¼ cup dried fruit, ¾ cup or 6 oz. 100% fruit or vegetable juice, ½ cup cooked or canned dried peas or beans.
Obesity Trends* Among U.S. Adults
1992 1995
1998 2001
Source: Mokdad A H, et al. J Am Med Assoc 1999;282:16, 2001;286:10.