bee venom therapy · from bee venom to apitoxin a chromatographic study done in 2001 at chemistry...

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© Th. Cherbuliez, M.D. & A. Kochan, M.D

Bee Venom & Apitoxin

Version:

Th. Cherbuliez, MD & A. Kochan, MD

CMACC 2012 – Portland, OR

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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx

Bee venom and Apitoxin Comparisons : list of topics - page 1

Ease of keeping &

transporting

Dilutions possible

Acceptability to patient

Can be injected in hard tissue

With procaine, is not allergenic

Apitoxin Bee venom

Specialized enterprise

Variable dosages possible

Acceptability to patient requires

working with

Can be injected in hard tissue to a

degree

Trusting relation may decrease risks.

Injectable at any depth Depth limited to stinger’s

size

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Bee venom and Apitoxin Comparisons : list of topics – page 2

Can be spread over large area

Specific molecule, identified action

Has small variability in potency

Can be used in research

Pain control, mixed with anesthetic

Can be spread over large area

Synergy of products and effects

Can be used in research

Pain control with different stings

Has always variability in composition

Apitoxin Bee venom

Loss of the “Energetics” of the bee “Energetics” conserved

Research re relative afficacy of Apit. No research relative efficacy of BV.

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From Bee venom to Apitoxin a chromatographic study

Done in 2001 at Chemistry Department of the University of Louvain

in Brussels, Belgium

Conducted by Prof. R. Domerego of the Apitherapy Commission of

Apimondia

Comparison of fresh bee venom with Apitoxin

Description of the differences and inferences about their clinical

meaning.

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From Bee venom to Apitoxin subtitle

Apitoxin from Michael Simics

Collection of fresh venom

Macerate of stingers in water 6 hours, with the presence of esterase

Without esterase the transformation takes 6 to 9 months

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Chromatography Collection Apparatus 2001

porous cylinder, with

Bees stinging tight junction

to Chomatograph

vacuum

warm water 80°

dry air 30° C

support

valve

Flow meter

tubing

Cool to - 90° C

T

°

© Apimondia Commission of Apitherapy Version:

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Chromatography-1 fresh Venom 140 bees

Compare 5,7,8,9,17,18,& 20 with apitoxin

34 spikes recorded in venom

4 & 6 represents 60% by mass

in fresh venom

© AAS & Apimondia Commission of Apitherapy Version:

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Chromatography-2 Apitoxin (M. Simics): 100 bees

very decreased ~ Disapeared New: R1,R2,R3,R4,R5 & R6

5 & 8

17,18 & 20

4

6


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Chromatography-3 macerate of 50 stingers for 6 hours

very decreased corresponding Generally decreased

17,18 & 20

5 & 8


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Chromatography Comparison Bee Venom / Apitoxine

© AAS & ApimondiaCommission ofApitherapy

34 spikes recorded in fresh bee venom

Spikes #4 & #6 most important in venom: 60% by weight

Spike #6 is at 6% in graphs 1 & 2 and 2% in graph 3

Spike #4 disappears in macerated venom sacs: graph 3

Spikes #5,7,8,9,17,and 20 disappear in graphs 3 & 4

Five new spikes appear in graphs 3 & 4

R1≈ D4, R2 ≈ D5, R3 ≈ D6, R4 ≈ D7 and R6 ≈ D9

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From Bee Venom to Apitoxin

Version:

Major chemical transformation of the volatile components are

observed from venom to apitoxin and macerate.

The esters present in the venom are absent in the apitoxin and in

the macerate. Therapeutically, their action is antispasmodic,

calming, tonic, anti-arrhythmic.

Apitoxin and sting macerate are quite similar.

New acids appear in the apitoxin and in the macerate.

Instability of the apitoxin and the macerate.

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Bee Venom Consultation One time Consultation; part 1

In was treated with 30 units (3000µg) of Apitoxin up to this consult

Man age ≈40; diagnosis MS; the “virulent” kind that kills in six months

I gave him 24 bees all on his back from coccyx to C7 (3600µg)

weight equivalent

He came on a stretcher in a light coma; he grunted when I talked to

him to his ear and in a loud voice

20 minutes later, he was eating an apple and talking with his family.

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Bee Venom Consultation One time Consultation; part 2

...with the consent of the family I gave him the bee stings

What I did:

What I caused factually to happen:

I related to him to the extend he was available: minimal, then...

He received a cocktail of substances that his system had never

experienced and that was new by the richness of essential oils

contained in the venom

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Bee Venom Consultation Reflection; part 3

The referring MD told me, had never seen such results

Look at the changes in his capacity to relate:

He related to his human environment:

He related to his physical environment:

He related to his body:

The change did not last. My treatment had no follow-up

Patient died two months later.

mother and young son

he was hungry

he ate an apple

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Apitoxin for Scarring

History

11/06/08 lumpectomy for CA of breast

12/12/08 mastectomy and clean-up of scar

01/08/09 Ist visit a lot of scarring and pain and

abduction limited to 90 degrees

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01/08/09 Ist visit a lot of scarring and pain and abduction limited to 90 degrees

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01/08/09 Ist visit a lot of scarring and pain and abduction limited to 90 degrees

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Treatment Course

01/08/09 0.5cc 25% Apitoxin 9 sites

01/09/09 0.5cc 25% Apitoxin 14 sites

Abduction 110 deg.

01/22/09 0.6cc 15 sites

01/29/09 0.6cc 15 sites 02/05/09 0.8cc 16 sites

Abduction 175 deg.!

12 more visits using 1cc or more completed on 06/12/09.

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06/12/09 Treatment completed

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Bee Venom Collector from Bulgaria

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INJECTABLE BEE VENOM

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END

bee venom therapy · from bee venom to apitoxin a chromatographic study done in 2001 at chemistry...

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