St engthening and E pansion of

CP007

Strengthening and Expansion of Postgraduate Research Capabilities for the

Development and Production of Inexpensive i k d l iLivestock and Poultry Vaccines

Dr. Md. Bahanur Rahman (SPM)Dr. Md. Tanvir Rahman (ASPM)

Bangladesh Agricultural University

Department of Microbiology and Hygiene: lAt a Glance

• Established: 1961• Established: 1961

• Degree offered: PhDPhDMS in Microbiology MS in Veterinary Public Health & Food Hygieney yg

• Courses offered at undergraduate level: Faculty of Vet. Science: 12 courses (22 Cr)Faculty of Anim. Husbandry: 4 courses (6 Cr)F lt f A il E & T h 2 (3 C )Faculty of Agril. Eng. & Tech.: 2 courses (3 Cr)

Departmental Teachers’ StrengthDepartmental Teachers’ Strengthp gp g

Professor A i t P f

6 Associate Professor Assistant Professor

Lecturer

841Lecturer

Total119

Departmental PG students Departmental PG students

PhD 6Current PG student enrolment

PhD MS

6 40

Total 46Total 46

PG degree completedPG degree completedPhD MS

10 384

Total 394

FundingFunding

• Funded by:unded by: World Bank (WB)World Bank (WB)•• Allocated amount:Allocated amount: BDT 297 7BDT 297 7 laclac•• Allocated amount:Allocated amount: BDT 297.7 BDT 297.7 laclac•• Expenditure: Expenditure: BDT 219 BDT 219 laclac (74%)(74%)•• Implemented by:Implemented by: Higher Education Quality Higher Education Quality

Enhancement Project (HEQEP),Enhancement Project (HEQEP),UGCUGC

Specific objectives

Improvement of MS and PhD research facilities

Procurement of state-of–art equipment andinstruments to enhance scientific capabilities ofthe relevant laboratories

E h t d i t f f i l Enhancement and improvement of professionalskills and research capabilities of academic staff,MS and PhD students

Improvement of IT facilities for PG students

Evaluation of quality safety potency and efficacy Evaluation of quality, safety, potency and efficacyof developed vaccines

Public-private partnership (PPP) establishment Public private partnership (PPP) establishment

Overall progress of the projectMajor Activities Present SituationRenovation and modernization of 6 laboratories, Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) and the Dept.Enrolment of MS and PhD students

Completed (6 Labs, 4 labs in LPVRPC, corridor, walls, ceiling, Enrolment of MS and PhD students

Local training floors)

Procurement and Installation of equipment / instruments and application in research

Completed (19 equipment & pp q pinstruments ) and research is in progress

IT facilities of the PG students Internet and bioinformatics facilities with PC for PG students

Vaccine Seeds, seed repository, inexpensive i d l t

Primary trial of 4 i d t d dvaccines development

Establishment of Reference Laboratory

vaccines conducted and genomic analysis in progressMolecular Microbiology y gy& Tissue culture Ref Lab established

Vaccines’ quality, safety, potency and efficacy

Molecular Microbiological Research F iliti D l dFacilities Developed

• Molecular characterization of bacteria and virus targeting their DNA and RNA using PCR and RT-PCR approach, cloning and sequencing etcE t bli h t f M d Ti C lt f iliti• Establishment of Modern Tissue Culture facilities for isolation, identification and characterization of animal and poultry viruses

• Installation of Cell imaging and documentation systemDNA d RNA i li ti d d t ti• DNA and RNA visualization and documentation system

• Protein analysis system e.g., SDS-PAGE andProtein analysis system e.g., SDS PAGE and Westerm blotting

Renovation of the Department and Departmental laboratories

Major Renovation WorksMolecular Bacteriology labMolecular Virology labo ecu a o ogy abImmunology LabVaccinology LabVaccinology LabTissue culture Lab Food microbiology LabFood microbiology Lab

4 Labs of LPVRPC including corridorsRenovation of DepartmentalRenovation of Departmental Corridors, Floor, Walls, Ceiling, glass doors etc

Laboratories Renovation

Lab before HEQEP Project Lab after HEQEP Project

Laboratories RenovationLaboratories Renovation

Lab before HEQEP ProjectLab before HEQEP Project

Lab after HEQEP Project

Laboratories Renovation

Lab before HEQEP Project Lab after HEQEP Project

Laboratories Renovation

Lab after HEQEP ProjectLab before HEQEP Project

Laboratories RenovationLaboratories Renovation

Lab after HEQEP Project

Departmental Renovation

Corridor before HEQEP Project Corridor after HEQEP Project

Renovation of Livestock and Poultry Vaccine

Research and ProductionResearch and Production Centre (LPVRPC)

LPVRPC R t dLPVRPC Renovated

LPVRPC after HEQEP Project

ll i f fInstallation of state-of-art Equipment forEquipment for

Postgraduate Researchg

Equipment Installed80 C d f Refrigerated Micro-centrifuge-80 C deep freezer Refrigerated Micro centrifuge

machine

Shaker incubatorAutoclave

Equipment InstalledI t d i W t b th h k i b tInverted microscope Water bath shaker incubator

IT and informatics facilities PhotocopierIT and informatics facilities Photocopier

Equipment InstalledHigh speed Refrigerated Centrifuge

Gel documentation systemHigh speed Refrigerated Centrifuge machine

Bi l i l f bi Cl II Micro ovenBiological safety cabinet Class-II Micro oven

Equipment InstalledEquipment InstalledDeionized water plantCO2 incubator

Equipment InstalledThermocycler ( PCR machine) Bacteriological shaker incubator

Equipment Installed

SDS-PAGE apparatus Electrophoresis apparatus

Equipment InstalledEquipment InstalledTrinocular Microscope

A li ti f St t fApplication of State-of-art Equipment forart Equipment for

Postgraduate Researchg

Equipment in Use

Equipment in UseEquipment in Use

Equipment in UseEquipment in Use

Equipment in Use

Equipment in UseEquipment in Use

Equipment in UseEquipment in Use

Equipment in Use

Equipment in UseEquipment in Use

Equipment in UseEquipment in Use

Equipment in UseEquipment in Use

Old Equipment RepairingOld Equipment Repairing

Old Equipment RepairingOld Equipment Repairing

TrainingTraining

TrainingTrainingTraining on Number ofTraining on Number of

participants

Power point presentation 100Power point presentation 100

Equipment Demonstration 45

Application of equipment for research

42

Technician Training 10

TrainingTraining

TrainingTraining

Training

Certificate given to the trainee

V i d V iVaccine and Vaccine Seeds De elopmentSeeds Development

Vaccine Developmentp

• Inactivated Trivalent FMD vaccine• Inactivated Trivalent FMD vaccine • ND Killed vaccine • Oil adjuvanated killed Fowl Cholera• Oil-adjuvanated killed Fowl Cholera

vaccine • Oil-adjuvanted killed HS vaccine• Oil-adjuvanted killed HS vaccine

BEI Inactivated Trivalent FMD Vaccine Development

Field sample collection for FMDV isolation

FMD Seed virus development

FMDV infected BHK-21 cells

Sero-typing of FMDVSero typing of FMDVFMDV serotypes -O, A and Asia-1 have been isolated, adapted in BHK-21 cell and finally identified by sandwich ELISA and RT-PCR for Master Seedand finally identified by sandwich ELISA and RT PCR for Master Seed development

FMDV sero-types O, A and Aisa-1 seedsFMDV sero types O, A and Aisa 1 seeds

Field trial of Trivalent FMD vaccinee d t a o a e t acc e

Oil adjuvanated Fowl CholeraOil adjuvanated Fowl Cholera Vaccine

Oil adjuvanated Fowl cholera vaccine d l tdevelopment

Pasteurella multocida organismsPasteurella multocida onto blood agar plate

Pasteurella multocida organisms isolated from infected chicken

Newcastle disease Killed vaccine developmentdevelopment

NDV adapted in BHK cell

NDV infected BHK-21 cell showing Uninfected BHK-21 cell typical cytopathic effect (CPE)

Detection of BHK-21 cell adapted NDV by RT PCRby RT-PCR

Lane: 6 F gene 385 bp

Lane: 7 Ladder (100-1031 bp)

Lane: 4 Control

Lane: 5 FFOP-gene 532 bpLane: 6 F-gene 385 bp

Lane: 3 Ladder (100-1500 bp)

RT-PCR detection of NDV targeting Fusion Gene (385 bp) and FFOP gene (532 bp)

Oil Adjuvanated HemorrhagicOil Adjuvanated Hemorrhagic Septicemia Vaccine

Pasteurella multocida

Scientific BreakthroughScientific Breakthrough FMDV serotypes-O, A and Asia-1 isolated, yp

identified, adapted in BHK-21 cell NDV adapted in BHK-21 cell

Experimental BEI inactivated trivalent FMD killed ND FC and HS vaccinesFMD, killed ND, FC and HS vaccines vaccine developed

Primary trial of these vaccines completed & Primary trial of these vaccines completed & second trial in progress

G i l i i Genomic analysis in progress

Mi bi l R itMicrobial Repository DevelopmentDevelopment

Microbial RepositoryMicrobial Repository

D l t d ti f•Development and preservation of bacterial and viral vaccine seeds

•Establishment of bacterial and viral strain repository from filed isolates

Microbial Repository

1901 bps

574 bps

Amplification of Hexon Amplification of 16s RNA gene ofAmplification of Hexon gene of EDS-76 virus

Amplification of 16s RNA gene of multi-drug resistant E. coli from chicken

Microbial Repository

Pseudomonas aeruginosaStaphylococcus aureus

500 bp 540 bp

Bacterial RepositoryBacterial Repository

Lab VisitsLab Visits

Laboratory Visit

Laboratory Visit

Laboratory Visity

Field VisitField Visit

Field Visit and vaccination campaignField Visit and vaccination campaign

Field Visit and vaccination campaign

Local and International Collaboration

Local CollaborationLocal Collaboration

FAO, Bangladesh

Department of Livestock Services (DLS)

Phenix Hatcher Ltd, Gazipur

International Collaboration EstablishedEstablished

Dr Roman Dziarski Dept of Microbiol & ImmunolDr. Roman Dziarski, Dept. of Microbiol. & Immunol., Indiana Univ., School of Med. USA

Dr Z Kornelius Associate Professor Dept of ProteinDr Z. Kornelius, Associate Professor, Dept. of Protein Evolution, Max Planck Institute, Germany

Prof Shi Tung Liu Mol Microbial Genetics Lab Dept ofProf. Shi-Tung Liu, Mol. Microbial Genetics Lab., Dept. of Microbiol & Immunol., Chang-Gung Univ., Taiwan

Dr Monica Cagiola Istituto Zooprofilattico SperimentaleDr. Monica Cagiola, Istituto Zooprofilattico Sperimentale dell'umbria e delle marche via g. salvemini, 06100, Perugia, Italy

Impact on Higher Studies and Research

Impact on Higher Studies and Research• Higher education quality and innovative research capability

developed

• Modern laboratories established and in use• Modern laboratories established and in use

• State-of art equipment procured , installed and in use

• Ultrapure grade chemicals & reagents procured and using for research

• Molecular microbiology, Tissue culture , vaccinology andMolecular microbiology, Tissue culture , vaccinology and immunology research facilities developed

• International & national collaboration established

• Establishment of PPP for production and distribution of vaccines in progress

T h & PG d f h D h F l ll h• Teachers & PG students of the Dept., the Faculty as well as the University benefited from implementation of this project

Acknowledgment• World Bank• Ministry of Education• Ministry of Education• UGC• HEQEP• HEQEP• BAU • Faculty of Veterinary Science BAU• Faculty of Veterinary Science, BAU• Dept. of Microbiology & Hygiene, BAU• FAO Bangladesh• FAO, Bangladesh• Department of Livestock Services (DLS)

Thank you all from the Department of Microbiology and Hygiene, BAU

THANK YOUTHANK YOUTHANK YOU THANK YOU