Visit us at www.Lctech.de!

SUPERIORITY BY ADAPTATION

Automated

Sample Preparation:SPE –

GPC -

EVAporation

What

LCTech does

supplies innovative products and methods for the preparation and analysis of food, feed and environmental samples.

Mission

THE CLEANER THE EXTRACT –

THE BETTER THE RESULT

Our

misson:

This system is designed for sample

preparation

mainly in food, feed and environmental laboratories,

but can be used in any lab in which GPC and/or any type of SPE columns are used for clean-up

FREESTYLE System -

Introduction

FREESTYLE –

The

Concept

EVAporation

SPE module

GPC module

FREESTYLE BASIC

FREESTYLE is

a system

which

is

based

on FREESTYLE BASIC and can

be

modified

with

modules

… in all imaginable combinations

Valves, syringe pump & others on top of y-axis (reduces dead volume in tubing)

FREESTYLE BASIC

FREESTYLE BASIC

xyz-robotic system

Flexbile robotic area; the appropriate „area“ can be created at any time by choosing the right racks

Rinsing port for needle

SPE Module

FREESTYLE Modules

SPE module as a 2nd z-axis

Universal manipulator for all standard SPE-/ Immuno affinity - (IAC) and Glass columns (e.g. LCTech columns) from 1 to 15 mL

Control unit for SPE module

FREESTYLE BASIC SPE Module

FREESTYLE Modules

GPC module

FREESTYLE Modules

Injection valve

High precision, double piston GPC-pump

Exchangeable sample loop

GPC-column: different sizes for all known official methods availableaccessory; not

included

in the

module

FREESTYLE BASIC

FREESTYLE Modules

GPC module

EVAporation module

FREESTYLE Modules

Heart

of the

EVAporation

system:EVAporation chamber with sensors

Integrated vacuum pump, heater & electronical devices

Solvent recovery/ solvent recycling

Water for temperature control of the vacuum chamber

FREESTYLE BASIC

FREESTYLE Modules

EVAporation Module

FREESTYLE –

adapted

to applications

Typical

combination

of different modules:

FREESTYLE BASIC + GPC + EVAporation:

For sample preparation (clean up) for pesticides / PAH and all other analytes in fatty matrices which have to be cleaned up before transfering to analytical instruments (eg. GC or LC).

Sample preparation for soil analysis

For a faster, reliable, more reproducible and more comfortable handling of such samples customer can choose a higher level of automation: FREESTYLE BASIC

with GPC module

for clean-up and EVAporation

module

FREESTYLE BASIC

FREESTYLE Modules

EVAporation ModuleGPC module

FREESTYLE –

modified

for

applications

Typical

combination

of different modules:

FREESTYLE BASIC + SPE + EVAporation:

Automation of SPE – Handling in all kind of labs

For a faster, reliable, more reproducible and more comfortable handling of such samples customer can choose a higher level of automation: FREESTYLE BASIC

with SPE module

and EVAporation

module

FREESTYLE BASIC SPE-Modul EVAporation Module

FREESTYLE –

modified

for

applications

FREESTYLE –

fully

automated

FREESTYLE BASIC

SPE-Modul EVAporation ModuleGPC-Modul

SUPERIORITY BY ADAPTATION

SUPERIORITY BY ADAPTION

Your

advantage

The

unique

design

Sorry, FREESTYLE does

not

offer 50 or

more

platforms!

FREESTYLE has just one,

but you

can create this one

according your needs, your demands, at any

time

(no service or tools needed)

Racks can be individually positioned by hooking them into a toothed plate

Racks –

the

key

for

flexibility

This pictures shows how it works

frame

tray with vials

= rack

Frame can be used for different kinds of vials

Tray can be taken out of frame and prepared independently

Racks –

the

key

for

flexibility

for vials from 1.0 mL to 1000 mL (e.g. standard flasks, round bottom flasks, TurboVap, Büchi, test-tubes…)

racks can be cooled or heated

With protection for light sensitive samples

Vials and flasks can be sealed with septa

= You can choose between r.a. 30 different racks. And if you cannot find the appropriate rack we are sure that we can design it. Ask LCTech!

Racks –

the

key

for

flexibility

Powerful Software

Selection of individual

modules

or

combination

of different functions within a single method

(eg. SPE +EVA or GPC+EVA)

no hardware

changes

necessary!

Allows intuitive handling

and easy usage in daily routine

Indicates most of the logical failures done by the customer

choosing the wrong parameters

Always included in the purchase price of modules

Powerful Software –

Main Window

FREESTYLE™

–

system

features

Integral system features

Extensive safety devices: - Possibility of immediate emergency stop

in case of danger or to prevent any dangerous situation

- Collisions recognition

with the possibility to proceed without loss of sample

All liquid containing tubing is located outside

the instrument, thus easily accessible and strictly separated from any electric wiring

Plausibility check

of software input

Software is always included

in the modules

Standards of development and production & safety guidelines

Developed and produced at highest level according to Machine Directive 2006/42/EC of the European Parliament and of the Council of 17 May 2006 on machinery, and amending Directive 95/16/EC (recast), including CE certification.

Developed and produced according to ISO 9001:2008.

CE labelled safety guidelines

FREESTYLE™ - Standards of production

For all standard SPE-cartridges/columns from 1 mL up to 15 mL

For all standard Immunoaffinity columns (1 mL/3 mL) IAC-columns can be stored in the rack sealed with a tight cap, so buffer does not flow out. IAC remains „fresh“ until you decide to use it

For glass columns for max. dimensions 105 x 17,5 ID (length x diameter)

SPE Module

SPE Module

Positive pressure

for all steps in which liquids are used

One rack

for all kind of columns: reusable adapters allow an easy to change and mixed setup for all kinds of columns

Columns can be moved to any

place in the robotic area

Standard three solvents, upgrade option up to six

solvents

Software allows the transfer of nearly any manual method straight to an automation

SPE Module & handling of SPE cartridges/SPE columns

SPE Module

Method

Development

Move next step from the basic tools with drag & drop to second column

The default button remains in the basic tools and can be choosen again

The collected steps in the second column build the method

Each button there can be individually changed and adopted by using the „bars“.

SPE Module

Software: SPE method

Application:determination

of the

Hydrocarbon

Oil Index in water/soil

according

to EN ISO 9377-2 (H53 Method)

For this very common routine application a special rack is used

36 samples can be handled in sequence

If desired FREESTYLE system (Evaporation module necessary) can evaporate them right away down to ≤

1.0 mL, sample can be

transferred in GC-Vial: ready for analysis

SPE Module

EVAporation

at its

best!

EVAporation Module

All necessary

items

are

integrated (only chiller for solvent recovery additionally needed)

Heart of the system: EVAporation chamber

frequency controlle diaphragm pump (Vacuubrand)

all sensoric items for detection of final volume

heating system with two zones a) with water for fast EVAporation b) electrical heater for the extended cone

for very sensitive evaporation

EVAporation

at its

best!

EVAporation Module

You

can

use

these

unique

and unbeatable

functions:

automated EVAporation down to a final volume

final volume can be choosen between 0.5 mL and 5.0 mL

final volume can be set by software; no manual adjustment of the sensors!

final volume can be set individually for each method = that means each sample in a sample list can have a different final volume

EVAporation either by vacuum/heat or by nitrogen/heat (option) for very sensitive samples

EVAporation

at its

best!

EVAporation Module

More

of these

unique

and unbeatable

functions:

solvent exchange (fluid/fluid or dryness/resolved)

360° C rinsing of the EVAporation chamber

Concentrate can be fractionated

Transfer of the concentrated sample with the robotic needle and not aspirated through a tube

Option: the whole system can be charged with nitrogen

Last but

not

least:

automated calibration of the EVAporation chamber at any time for reliable and high precision final volume

EVAporation

method

development; what

you

can

use

and modify:

Sample aspiration

Vacuum process

Blow-down with nitrogen or compressed air

Sample withdrawal from vacuum chamber

Solvent exchange

Fill-up and aliquotation

Vacuum chamber rinsing

EVAporation module

EVAporation

at its

best!

EVAporation Module

Software: EVAporation method

EVAporation

at its

best!

Double piston pump, esp. modified for low pressure applications (esp. with the appropriate pressure sensor)

Flow rate from 0.1 mL to 50 mL/min

GPC columns: - different dimensions

and

- different solvents for all official sample clean up methods worldwide

GPC Module

GPC method

development

(1):

5 different possibilites of injection

for all known applications:

- from sample loop overfill (traditional clean up for pesticide analysis)

- up to quantitative transfer by several injections from one glass (incl. 360° spray rinsing)

- and collection of these runs in one flask (dioxin-analysis)

Definition of forerun, main run

(total, partial or sequential fractionation) and tailings

Number and size of sample and collect vials

Depth of needle immersion

GPC Module

GPC method

development

(2):

Column injection counter

Flow rate

(GPC module)

Min. and max. pressure shut-off

Sample aspiration speed

(important for solvents like DCM)

Column type

and specification, respectively

UV Detector

data acquisition (yes/no)

GPC Module

GPC Module

Software: GPC method screen; the whole adjustment of a GPC method can be done in this easy to handle screen.

FREESTYL -

General Accessories

Barcode Reader- Reading width 65 mm- With USB connection cable

FREESTYLE -

General Accessories

Liquid level

switch

for organic solvent (e.g. for waste bottle)

Check of liquid level of waste bottle: Interruption of process, if bottle is empty / full (displayed in software)

Upgrade: 3 to 6 solvents:- Highly recommended

for SPE applications

Blow-down

function

for

nitrogen

and compressed

air:- necessary for usage of nitrogen/air for evaporation- and drying of columns in SPE-function;

FREESTYLE -

Accessories

for

SPE and EVA

FREESTYLE –

Accessories

for

GPC module

UV Fixed Wave Length Detector (254nm) for

GPC column

performance

test; necessary software is integrated

in the software package for FREESTYLE-systems

FREESTYLE -

SUPERIORITY BY ADAPTATION

Flexibility

on highest

level:

FREESTYLE BASIC+ GPC-Module+ EVAporations-Module+ SPE-Module

In one sample list, there can be combined any method totally independent from each other:

e.g.: 1. run: GPC2. run: GPC+ EVA3. run: GPC + EVA + SPE4. run: EVA

There is no hardware modification necessary at any time!

Where

to find us

Bahnweg 4184405 DorfenGermany

More about FREESTYLE-systems on www.freestyle-robotic.com orvisit us at www.LCTech.de!

Dorfen, Home of