ascentis express 5 μm columns with fused-core particles · pdf fileto any site or cro with...

8
Achieve More Efficiency With Less Backpressure Leverage Benefits on Any HPLC System Utilize C18 and F5 Phases Maximize Performance Ascentis ® Express 5 μm Columns with Fused-Core ® Particles Outperforms Popular 3 μm and 5 μm Columns

Upload: dodang

Post on 06-Feb-2018

243 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

Achieve More Efficiency With Less Backpressure

Leverage Benefits on Any HPLC System

Utilize C18 and F5 Phases

Maximize Performance

Ascentis® Express 5 µm Columns with Fused-Core® ParticlesOutperforms Popular 3 µm and 5 µm Columns

Page 2: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

2 sigma-aldrich.com/express

Asce

ntis

Expr

ess 5

μm

Col

umns

ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832

Ascentis Express 5 µm columns provide a new choice for improving the performance of traditional HPLC systems. Ascentis Express provides the benefits of high speed and high efficiencies without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures, Ascentis Express 5 µm can benefit conventional HPLC users with no drawbacks.

Ascentis® Express 5 µmAchieve Faster Separations With No Backpressure Concerns

“With the addition of the 5 micron particle to the Ascentis Express product line, end users can now boost the performance of their legacy HPLC systems and effortlessly transfer methods to any site or CRO with no concerns of incompatibility. The Ascentis Express product line is scalable from UHPLC to legacy HPLC systems. Furthermore, these columns provide researchers interested in ultra-high resolution HPLC the ability to reach new levels of resolution through column coupling, due to their low back pressure.”

Features at a Glance

•High e�ciencies

•Low backpressures

•Same instrument – no change of methods or sample prep

•Faster method development

•C18 and F5 phases

•Rugged design

•Outperforms popular 3 μm and 5 μm columns

Page 3: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

3

Ascentis Express 5 μm Colum

ns

0.5 µm

1.7 µm2.7 µm Solid Core

0.6 µm

3.3 µm~5 µm Solid Core

Small Diffusion PathThe new Fused-Core particle consists of a 3.3 µm solid core and a 0.6 µm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.6 µm) compared to conventional fully porous particles. The short diffusion path reduces axial dispersion of solutes and minimizes peak broadening. In fact, Ascentis Express 5 µm columns are able to achieve greater speed and efficiency than any other 5 µm particle based column. This means that Ascentis Express 5 µm becomes the standard column for all of your 5 µm based methods.

Beyond the new standard column for all 5 µm based methods, Ascentis Express 5 µm is an excellent choice for bioanalytical LC/MS methods. The Ascentis Express 5 µm excels under high flow rates and the high throughput demands of these methods. Furthermore, the large particle format provides an extremely rugged HPLC column.

C18 and F5 (PFP) PhasesAvailable in 17 dimensions from 2 cm x 2.1 mm I.D. to 25 cm x 4.6 mm I.D. and two introductory phases, C18 and F5, the conversion of current methods is seamless.

Key benefits of C18 and F5 phases include:

•Great combination of phases for method development

•Orthogonal selectivity

•High probability for separation

•F5 retentive both in RP and HILIC Mobile Phase conditions

Ascentis Express Fused-Core Particles

Leverage Easy Implementation on Any System

2.7 µm 5 µm

Page 4: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

4 sigma-aldrich.com/express

Asce

ntis

Expr

ess 5

μm

Col

umns

ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832

Maximize Performance Efficiencies With Fast Fused-Core

Fast HPLC with Shorter Diffusion Path

Fused-Core Particles Traditional Porous Particles

Consistent Bed Yields Sharper Peaks

Fused-Core Particles Traditional Porous Particles

Narrow Particle Size Distribution and Rugged Column Design

Fused-Core Particles Traditional Porous Particles

Page 5: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

5

Ascentis Express 5 μm Colum

ns

0 2 4 6 8 10Min

Ascentis Express C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=23,422 for last peak, 80 Bar

0 10 20Min

2 4 6 8 12 14 16 18

Popular C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=13,633 for last peak, 72 Bar

Summary of Efficiency, Backpressure and Speed

Column Efficiency (N) Toluene Backpressure (PSI) Runtime

Ascentis Express “5” C18, 15 cm x 4.6 mm 23,000 1200 8 min

Popular 5 µm C18, 15 cm x 4.6 mm 14,000 1100 16 min

Twice the Efficiency, Same Backpressure

23,422

13,633

1,200 1,0800

5,000

10,000

15,000

20,000

25,000

Ascentis Express 5 µm Popular 5 µm

Efficiency (plates)

Backpressure (PSI)

3 μmTotally Porous

5 μmTotally Porous

5 μmFused-Core

0 100 200 300 400

360

Plates/Pressure in bar

Best Plates per Pressure

The 5 µm Ascentis Express Fused-Core particle has more than double the number of plates/pressure of the 5 µm totally porous particles and four times the number of plates/pressure of the 3 µm totally porous particles.

Outperform Popular 3 μm and 5 μm Columns

Page 6: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

6 sigma-aldrich.com/express

Asce

ntis

Expr

ess 5

μm

Col

umns

ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832

Characteristics

Particle TypeShell

Thickness (µm)BET Surface Area (m2/g)

Average Pore Diameter (Å) Plates*

Pressure* (bar)

2.7 µm Ascentis Express Fused-Core 0.5 135 90 38,300 284

5 µm Ascentis Express Fused-Core 0.6 90 90 28,300 78

3 µm totally porous N/A 300 100 24,200 309

5 µm totally porous A N/A 300 100 14,600 100

5 µm totally porous B N/A 170 120 14,400 63

5 µm totally porous C N/A 450 100 15,300 120

* Plates and Pressure: reported for 1-chloro-4-nitrobenzene at the flow rate corresponding to the plate height minimum

Columns: 15 cm x 4.6 mm Mobile Phase: 50:50 ACN:water Temperature: 30 °C Injection: 1 μL Instrument: Agilent 1100 or Agilent 1200 with autosampler

10

100

1000

10000

1.00E+04 1.00E+05 1.00E+06

5 µm totally porous

3 µm totally porous

5 µm Fused-Core

t 0 (s

)

N (/)

Kinetic Plots of Various Particles Outperforms popular 3 μm and 5 μm columns under all conditions.

Compare Particle Attributes

Technical Conclusions

•Plates/Pressure: 5 µm Fused-Core particle has more than double the plates/pressure of 5 µm totally porous particles. – 4 times better than 3 µm particles

•5 µm Fused-Core particles can provide faster, more e�cient separations compared to 3 µm and 5 µm totally porous particles.

Page 7: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

7

Ascentis Express 5 μm Colum

ns

Get Started

Additional resources are available for helping you implement Ascentis Express HPLC Columns into your laboratory.

WebVisit sigma-aldrich.com/express for videos, product information, ordering and real time availability information.

EmailOur technical service staff is ready to answer questions.

EU: [email protected] US: [email protected]

In PersonA technical seminar can be arranged on-site or via the web. Request via [email protected].

I.D. Length C18 F5

2.1 mm 2 cm 50507-U 50603-U

2.1 mm 3 cm 50508-U 50604-U

2.1 mm 5 cm 50509-U 50605-U

2.1 mm 7.5 cm 50511-U 50607-U

2.1 mm 10 cm 50517-U 50612-U

2.1 mm 15 cm 50518-U 50613-U

2.1 mm 25 cm 50521-U 50614-U

3.0 mm 3 cm 50522-U 50615-U

3.0 mm 5 cm 50523-U 50616-U

3.0 mm 7.5 cm 50525-U 50619-U

3.0 mm 10 cm 50526-U 50622-U

3.0 mm 15 cm 50527-U 50623-U

3.0 mm 25 cm 50528-U 50624-U

4.6 mm 3 cm 50529-U 50625-U

4.6 mm 5 cm 50530-U 50626-U

4.6 mm 7.5 cm 50533-U 50627-U

4.6 mm 10 cm 50536-U 50628-U

4.6 mm 15 cm 50537-U 50631-U

4.6 mm 25 cm 50538-U 50632-U

Ordering Information

I.D. Length C18 F5

2.1 mm – 50539-U 50633-U

3.0 mm – 50541-U 50634-U

4.6 mm – 50542-U 50635-U

Ascentis Express 5 µm Columns Ascentis Express 5 µm Guard Cartridges, Pk of 3

Description Cat. No.

Holder w/EXP Titanium Hybrid Ferrule (cartridge not included)

53500-U

Ascentis Express Universal Guard Holder

Guard Cartridge

Holder

For more information about Ascentis Express, visit sigma-aldrich.com/express

Page 8: Ascentis Express 5 μm Columns with Fused-Core Particles · PDF fileto any site or CRO with no concerns of ... Ascentis Express 5 µm is an excellent choice for bioanalytical ... Mumbai:

Order/Customer Service (800) 325-3010 • Fax (800) 325-5052 Technical Service (800) 325-5832 • sigma-aldrich.com/techservice Development/Custom Manufacturing Inquiries (800) 244-1173 Safety-related Information sigma-aldrich.com/safetycenter

OSV11490-509521

1072

©2012 Sigma-Aldrich Co. LLC. All rights reserved. SAFC, SIGMA-ALDRICH and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Materials Technology, Inc. Supelco brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.

World Headquarters 3050 Spruce St.

St. Louis, MO 63103 (314) 771-5765

sigma-aldrich.com

Enabling Science to Improve the Quality of Life

Sigma-Aldrich® Worldwide Offices

ArgentinaFree Tel: 0810 888 7446 Tel: (+54) 11 4556 1472 Fax: (+54) 11 4552 1698

AustraliaFree Tel: 1800 800 097 Free Fax: 1800 800 096 Tel: (+61) 2 9841 0555 Fax: (+61) 2 9841 0500

AustriaTel: (+43) 1 605 81 10 Fax: (+43) 1 605 81 20

BelgiumTel: (+32) 3 899 13 01 Fax: (+32) 3 899 13 11

BrazilFree Tel: 0800 701 7425 Tel: (+55) 11 3732 3100 Fax: (+55) 11 5522 9895

CanadaFree Tel: 1800 565 1400 Free Fax: 1800 265 3858 Tel: (+1) 905 829 9500 Fax: (+1) 905 829 9292

ChileTel: (+56) 2 495 7395 Fax: (+56) 2 495 7396

People’s Republic of ChinaFree Tel: 800 819 3336 Tel: (+86) 21 6141 5566 Fax: (+86) 21 6141 5567

Czech RepublicTel: (+420) 246 003 200 Fax: (+420) 246 003 291

DenmarkTel: (+45) 43 56 59 00 Fax: (+45) 43 56 59 05

FinlandTel: (+358) 9 350 9250 Fax: (+358) 9 350 92555

FranceFree Tel: 0800 211 408 Free Fax: 0800 031 052 Tel: (+33) 474 82 28 88 Fax: (+33) 474 95 68 08

GermanyFree Tel: 0800 51 55 000 Free Fax: 0800 64 90 000 Tel: (+49) 89 6513 0 Fax: (+49) 89 6513 1169

HungaryTel: (+36) 1 235 9055 Fax: (+36) 1 235 9068

IndiaTelephone Bangalore: (+91) 80 6621 9400 New Delhi: (+91) 11 4358 8000 Mumbai: (+91) 22 4087 2364 Pune: (+91) 20 4146 4700 Hyderabad: (+91) 40 3067 7450 Kolkata: (+91) 33 4013 8000

Fax Bangalore: (+91) 80 6621 9550 New Delhi: (+91) 11 4358 8001 Mumbai: (+91) 22 2579 7589 Pune: (+91) 20 4146 4777 Hyderabad: (+91) 40 3067 7451 Kolkata: (+91) 33 4013 8016

IrelandFree Tel: 1800 200 888 Free Fax: 1800 600 222 Tel: +353 (0) 402 20370 Fax: + 353 (0) 402 20375

IsraelFree Tel: 1 800 70 2222 Tel: (+972) 8 948 4222 Fax: (+972) 8 948 4200

ItalyFree Tel: 800 827 018 Tel: (+39) 02 3341 7310 Fax: (+39) 02 3801 0737

JapanTel: (+81) 3 5796 7300 Fax: (+81) 3 5796 7315

KoreaFree Tel: (+82) 80 023 7111 Free Fax: (+82) 80 023 8111 Tel: (+82) 31 329 9000 Fax: (+82) 31 329 9090

LuxembourgTel: (+32) 3 899 1301 Fax: (+32) 3 899 1311

MalaysiaTel: (+60) 3 5635 3321 Fax: (+60) 3 5635 4116

MexicoFree Tel: 01 800 007 5300 Free Fax: 01 800 712 9920 Tel: (+52) 722 276 1600 Fax: (+52) 722 276 1601

The NetherlandsTel: (+31) 78 620 5411 Fax: (+31) 78 620 5421

New ZealandFree Tel: 0800 936 666 Free Fax: 0800 937 777 Tel: (+61) 2 9841 0555 Fax: (+61) 2 9841 0500

NorwayTel: (+47) 23 17 60 00 Fax: (+47) 23 17 60 10

PolandTel: (+48) 61 829 01 00 Fax: (+48) 61 829 01 20

PortugalFree Tel: 800 202 180 Free Fax: 800 202 178 Tel: (+351) 21 924 2555 Fax: (+351) 21 924 2610

RussiaTel: (+7) 495 621 5828 Fax: (+7) 495 621 6037

SingaporeTel: (+65) 6779 1200 Fax: (+65) 6779 1822

SlovakiaTel: (+421) 255 571 562 Fax: (+421) 255 571 564

South AfricaFree Tel: 0800 1100 75 Free Fax: 0800 1100 79 Tel: (+27) 11 979 1188 Fax: (+27) 11 979 1119

SpainFree Tel: 900 101 376 Free Fax: 900 102 028 Tel: (+34) 91 661 99 77 Fax: (+34) 91 661 96 42

SwedenTel: (+46) 8 742 4200 Fax: (+46) 8 742 4243

SwitzerlandFree Tel: 0800 80 00 80 Free Fax: 0800 80 00 81 Tel: (+41) 81 755 2511 Fax: (+41) 81 756 5449

ThailandTel: (+66) 2 126 8141 Fax: (+66) 2 126 8080

United KingdomFree Tel: 0800 717 181 Free Fax: 0800 378 785 Tel: (+44) 1747 833 000 Fax: (+44) 1747 833 313

United StatesToll-Free: 800 325 3010 Toll-Free Fax: 800 325 5052 Tel: (+1) 314 771 5765 Fax: (+1) 314 771 5757

VietnamTel: (+84) 8 3516 2810 Fax: (+84) 8 6258 4238

Internet sigma-aldrich.com