Apoptosis

Necrosis and Apoptosis

Apoptosis Morphological

Morphological

Necrosis Biochemical

Apoptosis Biochemical

Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin D (Lane 2). DNA Markers (M) were electrophoresed as a base pair reference.

DNA ladder

nucleosome

FACS DNA

Anexin V

Anexin V

Necrosis physical

Apoptosis physical

Physical significance

3. Detection of Apoptosis

• Morphological

• DNA electrophoresis

• DNA 断裂的原位末端标记 TUNEL 测定法， Terminal deoxynucleotid

yl transferase(TdT)-mediated dUTP end labeling ，末端脱氧核苷酸移换酶介导的 dUTP缺口末端标记测定法

• Flowmetery

3.Regulation of Apoptosis

⑴ Genes for proliferation

c-myc

ras

⑵ For survival

bcl-2

c-kit

⑶ proliferation inhibitors

p53

Rb

⑷ death promotors

Bax

ICE

4.p53 and apoptosis

2002 Press Release

Laureates

Ap-overview