Jerrone HansonBlock 4Ms. RussoEnzyme Catalysis Lab report

Enzyme Activity

Activity ObservationEnzyme Activity Bubbles.

Effects when tempreture rises Nothing Happens because it denatures at extreme temp.

When The catalysis is present It Bubbles.

The Baseline

VolumeInitial Reading 5ml

Final Reading 2.4ml

Baseline (which is the initial volume minus the final volume)

2.6ml

Class Data

Time Amount of H2O2

Decomposed in Trial 1Amount of H2O2

Decomposed in Trial 2

Average Amount of

H2O2

Decomposed10 Seconds 1.43 1.03 1.23

30 Seconds 2.23 .83 1.53

60 Seconds 2.03 1.83 1.93

120 Seconds 2.63 1.63 2.13

180 Seconds 2.83 2.43 2.63

This shows what happens to the enzyme at certain states or when it is exposed to certain environments or even when it comes in contact with certain things.

This is the Catalyzed Decomposition measurements.

The Collection of the class data which shows different seconds and measurements of the experiment that differs depending on the accuracy of the lab. The trial two(2) of the 30 Second reaction was reasonably low because of some error that might have happened in the experiment, such as the enzyme might have been weaker than usual, But overall, but observing the average amount that was decomposed chronologically from 10 seconds to 180, it is quite reasonable.

Class Data2 (Graph)

Class Average data (Graph)

Analysis

The purpose of this lab was to observe and understand the effects of changes in temperature,

pH, enzyme concentration, and substrate concentration on the reaction rate of an enzyme-

catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect

the rate of enzyme-catalyzed reactions. The data and graph shows that during the 180 seconds,

the rate of reaction was the fastest, which means that more Hydrogen peroxide was

decomposed at in the shortest amount of time.

There were two trials used for each time ranging from ten (10) seconds to one hundred and eighty (180) seconds. Notice that the amount of H2O2

decomposed increases with time, except for trial two (2) at thirty (30) seconds which the amount fell the lowest and this could be caused by errors in terms of measurement, time, environment exposure of the enzymes, etc.

By observing the outcome of the class’s average data, it made sense that as the time increased, the amount of hydrogen peroxide also increased. The rate was not totally consistent, but it never fell, it was always increasing as time increases.

Amount of Hydrogen Peroxide Decomposed (Trial 1 & 2)

Conclusion

The catalase enzyme helped in the reaction of decomposing hydrogen peroxide into water and oxygen. However, enzymes are very environmentally sensitive, so our group was able to see direct effects on the reaction when the catalase solution was out for a period of time and possibly, denaturing it and reducing the results of the reaction. As for the effect of time and the catalase on the rate of reaction, we were able to see a high rate of reaction toward the beginning of the reaction, but it slowly leveled out when more of the H2O2 had decomposed. This experiment could be improved by trying to be more accurate when measuring the reactants before they are added to the solution. Perhaps if the catalase was not out for as long, the experiment could have given a better result.