Incorporation of Antimicrobial Agents in Food Packaging Films

Agnescia Sera | 43850565

Food Bioprocessing

Problem: Food Contamination

Antimicrobial packaging Integration of antimicrobials agents into packaging materials

To kill or to inhibit the pathogenic and spoilage microorganism

Protein Damage

• Proteins are essential for the biological systems of life, any damage to these components causes the failure of essential functions such as energy production.

Cell Membrane Damage

• By disrupting the microbes membrane, the structural integrity of the microbe is compromised, which can cause essential nutrients to leak out and catastrophic structural failure.

Oxidative Damage

• Antimicrobials can cause increased levels of reactive oxygen species, which can cause damage to the internal systems of the microbe.

DNA Interference

• The genetic material of the bacteria is disrupted. Stopping the bacteria being able to replicate by blocking the copying of their genetic material, preventing reproduction

Conventional vs active packaging

Compositions

Antimicrobial agents

• Natural

• Plant source

• Animal source

• Microorganism source

• Chemical

• Benzoic acid

• Sorbic acid

Films

• LDPE, LLDPE, HDPE

• PET, Polyolefin EVA

• MC

• WPI, SPI

• Egg albumen

• Methyl cellulose

• Corn zein films

Antimicrobial agents Matrix film Microorganism References

Malic acid, nicin,

natamycin

Whey protein Penicillium aeruginosa,

Penicillium commune,

Penicillium chrysogenum,

L. monocytogenes, Y.lipolytica,

Lagaron et al., 2011

Bacteriocins Pea protein isolate,

hydroxypropylmethylcellul

ose, methylcellulose,

sodium caseinate

L. inocua Sancez-Gonzalez et

al., 2013

Grape seed extract, nisin,

EDTA

Soy protein E.Coli O175:H7, L.

monocytogenes, S. typhimurium

Perumalla and

Sunil, 2012

Potassium sorbate Wheat gluten Aspergillus niger, Fusarium

incarnatum

Ture, Gallstedt and

Hedenqvist, 2012

Propionic acid Chitosan E.coli, L. plantarum Leceta et al.,2013

Clove essential oils Clove, Cyprus, citronella Aspergillus niger, Bacillus

coagulans, Bacillus cereus

Goni et al., 2009

Edible antimicrobial packaging

Methods

Addition of sachets (pads) Incorporating directly into the packaging films

Coating of packaging with a matrix that acts as a carrier for antimicrobial

agent

Examples

99% reduction in bacteria within

2 hours

Extending the shelf life of

products

Effective against bacteria and

moulds

Evidences

C. Hauser and J. Wunderlich, Procedia Food

Science 1, 197-202 (2011).

http://www.biocote.com/blog/biocote-bacteria-

imaging/

Challenges Susceptibility to the high processing

temperature used to manufacturing

packaging polymers

Regulation issues

Antimicrobial packaging is a promising form

of active packaging to improve safety and

shelf life of products

The use of packaging materials is not meant

to be a substitute for good sanitation!!

Conclusions

References

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