anatomy of a pet food catastrophe

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  • 41WWW.CEN-ONLINE.ORG MAY 12, 2008

    THE PET FOOD contamination crisis of 2007 began quietly, with just a few cats and dogs becoming seriously ill or dying unex-pectedly. Regrettably, those first few vic-tims were followed by many more. Ul-timately, the incident led to massive recalls of pet food, multiple lawsuits against pet food suppliers that are only now being settled, and an inten-sive effort in labs throughout North America to track down the cause of the deaths. Even now, research con-tinues into the properties and health effects of the adulterants responsible for the calamity.

    As the case unfolded, pet food manufacturers recalled millions of containers of more than 150 brands of dog and cat food. There definitely was a bit of a panic among pet own-ers, especially when the names of all the foods were still coming out, and people werent sure if the food they were feeding their pets was going to turn up on the list, recalls Kristy Bennett, an associate veterinar-ian at Wheaton Animal Hospital in Ken sington, Md. While her practice treated only a few of the affected pets, quite a lot of veterinarians were see-ing cases left and right, she says.

    Animals that ate the adulterated pet food developed symptoms of kidney failure, including loss of appetite, vomit-ing, lethargy, frequent urination, increased thirst, and death. Stricken pets also had heightened levels of nitrogen compounds and phosphates in their blood and distinc-tive round, greenish brown kidney stones clogging their kidneys, says William H. Tolleson, a cell biologist at the Food & Drug Administration.

    FDA, which regulates the manufacture and distribution of animal food and drugs, logged more than 18,000 phone calls after

    the recalls were announced. This was the largest recall in FDA history, says Tolleson, who works in the agencys Na-tional Center for Toxicological Research

    (NCTR) in Jefferson, Ark. There were more calls to FDA over this is-sue than anything weve ever handled before.

    As many as 1,000 dogs and cats may have died in North America, according to Colorado State University veterinary pathologist Barbara E. Powers. She bases her estimate on a survey she helped conduct last year as president of the American Association of

    Veterinary Laboratory Diagnosticians.Within two months of the first cases,

    chemists, toxicologists, and other inves-tigators traced the deaths to two spe-cific adulterants in pet food ingredients: melamine and cyanuric acid.

    Melamine is used to make plastics, fertilizer, and other products but is not approved as an ingredient in human or animal food in the U.S. The compound emits a very high level of nitrogen when it is analyzed, according to Paul K. Hender-son, president and CEO of Menu Foods, one of the pet food manufacturers that unwittingly used a contaminated ingredi-ent. The reason this matters is that both

    the human and pet food industries routinely test for nitrogen as a way of confirming the desired protein levels in their raw materials.

    Why would the Chinese supplier do this? We believe it was to artifi-cially increase the apparent level of the protein, Henderson said last June during his companys annual meeting.

    Cyanuric acid is normally used to stabilize chlorine in swimming pools. The compound is a by-product of melamine synthesis and could have been an impurity in the melamine added to the pet food, Tolleson speculates.

    Sadly, the 2007 incidents might not have been the first. A 2004 out-break of kidney failure that report-edly struck thousands of dogs and cats in Asia originally was attributed to contamination of pet food ingredi-ents by fungal toxins. But University of Georgia veterinary pathologist Cathy A. Brown and colleagues re-ported in September 2007 that they had found compelling evidence in tissue samples from autopsies of af-fected pets that both outbreaks were caused by the same toxic chemical combination (J. Vet. Diagn. Invest.2007, 19, 525).

    The latest drama began unfolding in February 2007, when Canadas Menu Foods began receiving com-plaints about illness and death in cats

    that might have eaten the companys pet food. On March 15, Menu Foods notified FDA that at least 10 animals had died, says R. Duane Satzger, director of the organic

    SCIENCE & TECHNOLOGY

    ANATOMY OF A PET FOOD CATASTROPHE

    Investigators deployed an array of analytical tools while probing a wave of PET DEATHS in 2007

    SOPHIE L. ROVNER, C&EN WASHINGTON

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    MORE ONLINETo learn more about the case load and origins of FDAs Forensic Chemistry Center and the National Center

    for Toxicological Research, visit C&EN Online, www.cen-online.org.

    INNOCENT VICTIM

    Los Angeles veterinarian Pedro Cisneros examines Pebbles, a Yorkshire terrier that eventually died after eating contaminated pet food.

  • 42WWW.CEN-ONLINE.ORG MAY 12, 2008

    branch of the agencys Forensic Chemistry Center in Cincinnati.

    The next day, Menu Foods announced a recall of some of the dog and cat food it had manufactured between Dec. 3, 2006, and March 6, 2007, because of concerns over kidney failure in pets. The start date for the recall was later expanded back to Nov. 8, 2006. The firms customers, including Procter & Gamble and Nestl Purina PetCare, also began initiating their own recalls.

    Menu Foods discovered that production of the problematic food coincided with introduction of an ingredient from a new supplier. In late 2006, Menu Foods had added ChemNutra of Las Vegas as a source for wheat gluten, a concentrated vegetable protein used as a thickener or binding agent in pet food. Other pet food manu-facturers also purchased small quantities of wheat gluten from ChemNutra and ulti-mately initiated recalls as well. ChemNutra itself had acquired the wheat gluten from a Chinese supplier.

    AT THE TIME of the initial recall last year, the specific defect in the wheat gluten was a mystery, despite a battery of tests. Assays for minerals, heavy metals, ethylene glycol, pesticides, toxins formed by molds, and in-tentionally added toxins came up empty.

    Then on March 23, researchers at the New York State Food Laboratory said they had identified the rat poison aminopterin as a toxin in the pet food (C&EN, April 2, 2007, page 11). FDA was unable to cor-roborate the finding. But Daniel H. Rice, director of the New York lab, stands by the aminopterin identification. If lab findings dont support each other, its usually due to different methodology, he says. In this case, FDA instrumentation was different than ours. Rice notes his lab detected the aminopterin only at parts-per-billion lev-els, so it probably wasnt a factor in sicken-ing pets anyway.

    On March 30, FDA and Cornell Uni-versitywhose animal health experts had been enlisted in the investigation by Menu Foodsannounced that they had independently identified melamine as a contaminant.

    Cornell researchers had detected the melamine in urine and kidney samples from affected pets, and both Cornell and FDAs Forensic Chemistry Center (FCC) had detected the contaminant in the pet food.

    FCC made an initial tentative iden-tification of the melamine using a mass spectrometric (MS) technique that relies on the open-air ionization method known as direct analysis in real time (DART) (C&EN, Oct. 8, 2007, page 13). The group confirmed the finding with additional DART and gas chromatography (GC)-MS runs.

    The finding was a bit mysterious because the small amount of information available in the scientific literature indicated that melamine wasnt particularly toxic.

    FCC staff next ex-amined samples of the suspect wheat protein concentrate under a light microscope and separated out what ap-peared to be foreign par-ticles. Fourier transform infrared and Raman spectra of the particles, as well as DART and GC-MS, confirmed the presence of melamine. FCCs analysis also unearthed further tri-azine contaminants, particularly cyanuric acid. Satzger says that total adulterant lev-els in the protein concentrate ran as high as 25% by weight.

    The DART technique is much quicker than GC-MS because it requires no sample preparation, Satzger notes, but it depends on expensive equipment thats not widely available. Throughout April 2007, FCC scientists labored to develop a GC-MS method to rapidly screen pet food samplesone that could be replicated in other labs across the country with com-monly available instrumentation. One challenge involved finding a way to test for multiple triazines, which have widely varying solubilities, in a single run. FCC researchers working in collaboration

    with FDAs Pacific Regional Laboratory Northwest, in Bothell, Wash., found that a solvent system of acetonitrile, water, and diethylamine did the trick.

    During this same period, other FCC scientists were examining crystals from kidney tissue provided by FDAs Rockville, Md.-based Center for Veterinary Medicine, Satzger says. Raman mapping studies indi-cated the crystals could be melamine cya-nurate. Lab tests also showed that mixing melamine and cyanuric acid in water forms melamine cyanurate crystals.

    Meanwhile, Tolleson was gathering data about the toxicity of the pet food contami-nants using cultured cells from the immune system known as mac-rophages. He found that cyanuric acid by itself was only slightly toxic; by contrast, melamine and the combination of both compounds were toxic to the macrophages.

    In April 2007, Procter & Gamble toxicolo-gist George P. Daston showed that the triazine compounds are deadly to rats when consumed

    in combination, Tolleson says. Rats that ate the mixture developed the same kidney disorder seen in pet cats and dogs. Neither Daston nor other P&G representatives would comment for this story beca