GE HealthcareLife Sciences

Amersham™ Western blottingSelection guide

Western blotting selection guide

Product Quantity Fisher Scientific Cat. No.

Description

Sample preparationSDS-PAGE Clean-Up kit • • • 50 samples 45-001-738 Removal of interfering contaminants and concentration of total proteinMammalian protein Extraction buffer • • • 1 × 500 ml 45-002-169 Buffer for extraction of proteins from mammalian cells2-D Quant kit • • • 500 assays 45-001-732 Quantitation of protein amount using a colorimetric assay

Gel electrophoresisAmersham ECL™ Gel Box • • • 1 45-002-481 Horizontal electrophoresis system with virtually no assembly requiredAmersham ECL Gel 10%, 2 wells • • • Pack of 10 45-002-471 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 10 wells • • • Pack of 2 45-002-476 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 10 wells • • • Pack of 10 45-002-461 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 15 wells • • • Pack of 10 45-002-466 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 2 wells • • • Pack of 10 45-002-472 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 10 wells • • • Pack of 2 45-002-477 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 10 wells • • • Pack of 10 45-002-462 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 15 wells • • • Pack of 10 45-002-467 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 2 wells • • • Pack of 10 45-002-473 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 10 wells • • • Pack of 2 45-002-478 Precast horizontal gradient PAGE gel for protein electrophoresis

Amersham ECL Gel 4-12%, 10 wells • • • Pack of 10 45-002-463 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 15 wells • • • Pack of 10 45-002-468 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 2 wells • • • Pack of 10 45-002-474 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 10 wells • • • Pack of 2 45-002-479 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 10 wells • • • Pack of 10 45-002-464 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 15 wells • • • Pack of 10 45-002-469 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 2 wells • • • Pack of 10 45-002-475 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 10 wells • • • Pack of 2 45-002-480 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 10 wells • • • Pack of 10 45-002-465 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 15 wells • • • Pack of 10 45-002-470 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel running buffer • • • 10 runs 45-002-482 Pre-made 10 × concentrated Laemmli buffer for denatured protein electrophoresisEPS 301 Power Supply • • • 1 45-000-465 Power supply suitable for the Amersham ECL Gel systemDeep Purple Total Protein Stain • 25 ml 45-001-299 Rapid, ultra-high sensitivity fluorescent total protein stain. Suitable for both

protein gels and blots.Bromophenol Blue • • • 10 g 45-000-242 Dye for visualization of the migration of proteins in gel electrophoresis

MarkerLow-Range Rainbow™ Molecular Weight Markers • • 250 μl 45-001-593 Mr 3 500 to 38 000, seven separate proteins with five different visible colors, not

visible on chemiluminescence imageHigh-Range Rainbow Molecular Weight Markers • • 250 μl 45-001-592 Mr 12 000 to 225 000, eight separate proteins with six different visible colors, not

visible on chemiluminescence imageFull-Range Rainbow Molecular Weight Markers • • 250 μl 45-001-591 Mr 12 000 to 225 000, ten separate proteins with six different visible colors, not

visible on chemiluminescence imageAmersham ECL DualVue Western blotting markers • • 25 loadings 45-001-182 Detect proteins on gel and membrane as well as on film, Mr 15 000 to 150 000

with 3 bands visible on gel and blotting membrane and 7 bands visible on chemiluminescence image

Amersham ECL Plex Fluorescent Rainbow markers • 120 μl 45-001-594 Protein visible molecular weight markers, as well as fluorescent images using Cy3 and Cy5 channels. Mr 12 000 to 225 000

Amersham ECL Plex Fluorescent Rainbow markers • 500 μl 45-001-595 Protein visible molecular weight markers, as well as fluorescent images using Cy3 and Cy5 channels. Mr 12 000 to 225 000

Blotting paper3MM Chr 20 × 20 cm • • • 100 sheets 05-716-3V Blotting paper suitable for Western blot

Blotting sandwichProtran BA83, 0.2 μm blotting sandwich (8 × 7.5 cm) • • 10 units 09-925-109 Pre-cut Protran membrane and blotting paper to fit with the ECL Gel system for

Western blottingProtran BA85, 0.45 μm blotting sandwich (8 × 7.5 cm) • • 10 units 09-925-110 Pre-cut Protran membrane and blotting paper to fit with the ECL Gel system for

Western blotting

MembranesAmersham Hybond™ ECL (20 × 20 cm) • • • 10 sheets 45-000-839 Unsupported, 100% pure nitrocellulose membrane validated for use with all ECL

Western blotting systems and for all protein blotting applications.Amersham Hybond ECL (30 cm x 3 m) • • • 1 roll 45-001-929 Unsupported, 100% pure nitrocellulose membrane validated for use with all

ECL Western blotting systems and for all protein blotting applications. Amersham Hybond-LFP (20 × 20 cm) • 10 sheets 45-001-313 Low-fluorescent PVDF membrane optimized for fluorescent Western blotting applicationsAmersham Hybond-LFP (30 cm x 3 m) • 1 roll 45-001-315 Low-fluorescent PVDF membrane optimized for fluorescent Western blotting applications

Pre-cut to fit with the ECL Gel systemAmersham Hybond-P (20 × 20 cm) • • 10 sheets 45-000-841 Hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in

protein transfers and/or Western blotting applicationsAmersham Hybond-P (30 cm x 3 m) • • 1 roll 45-000-931 Hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in

protein transfers and/or Western blotting applications. Pre-cut to fit with the ECL Gel system

Chemiluminesc

ence/F

ilm

Chemiluminesc

ence/C

CD

Fluoresc

ence

Many types of products are available in other formats and pack sizes. Please visit www.fishersci.com for more information.

Transfer units Quantity Fisher Scientific Cat. No.

Description

TE 22 Mini Tank Transfer Unit • • • 1 45-001-953 Performs protein and nucleic acid transfers in less than one hour. Transfers four mini-gels up to 9 × 10 cm in about 1 l of buffer

TE 62 Transfer Unit • • • 1 45-001-962 Tank transfer for gels up to 15 × 21 cmTE 70 Semi-Dry Transfer Unit, 14 × 16 cm • • • 1 45-001-963 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hourTE 70 PWR Semi-Dry Transfer Unit, 14 × 16 cm • • • 1 45-001-782 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hour.

Built in power supply TE 77 Semi-Dry Transfer Unit, 21 × 26 cm • • • 1 45-001-968 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hourTE 77 PWR Semi-Dry Transfer Unit, 21 × 26 cm • • • 1 45-001-783 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hour.

Built in power supply EPS 2A200 Power supply • • • 1 450-01-974 Power supply suitable for Western blotting

Blocking agentAmersham ECL Blocking agent • • • 40 g 45-001-197 For blocking nitrocellulose and PVDF membranes to be used with e.g.

ECL Western blotting detection reagentsAmersham ECL Prime Blocking reagent • • • 40 g 45-001-216 For blocking nitrocellulose and PVDF membranes to be used with e.g.

ECL Prime and ECL Plex Western blotting detection reagents

Secondary antibodyAmersham ECL Mouse IgG, HRP-linked whole Ab (from sheep)

• • 1 ml 45-000-679 Secondary antibody for using with e.g. ECL or ECL Prime

Amersham ECL Human IgG, HRP-linked whole Ab (from sheep)

• • 1 ml 45-000-681 Secondary antibody for using with e.g. ECL or ECL Prime

Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)

• • 1 ml 45-000-682 Secondary antibody for using with e.g. ECL or ECL Prime

Amersham ECL Mouse IgG, HRP-linked F(ab)2 fragment (from sheep)

• • 1 ml 45-000-680 Secondary antibody for using with e.g. ECL or ECL Prime

Amersham ECL Rabbit IgG, HRP-linked F(ab)2 fragment (from donkey)

• • 1 ml 45-000-683 Secondary antibody for using with e.g. ECL or ECL Prime

Amersham ECL Plex™ goat-a-mouse IgG-Cy2 • 150 ug 45-0014-75 Cy-2 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy2 • 150 ug 45-0014-77 Cy-2 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy3 • 150 ug 45-0014-73 Cy-3 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-mouse IgG-Cy3 • 150 ug 45-001-208 Cy-3 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy5 • 150 ug 45-001-212 Cy-5 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-mouse IgG-Cy5 • 150 ug 45-001-211 Cy-5 conjugated secondary antibody for fluorescent detection

Detection reagentAmersham ECL Prime Western blotting reagent • • for 1000 cm2 45-002-401 Chemiluminescent detection of Western blotting using film or CCD camera,

low pg detection levelAmersham ECL Western blotting detection reagent • • for 1000 cm2 45-000-878 Chemiluminescent detection of Western blotting using film or CCD camera,

mid pg detection levelAmersham ECL Western blotting detection reagent • • for 4000 cm2 45-000-875 Chemiluminescent detection of Western blotting using film or CCD camera,

mid pg detection levelAmersham ECL Western blotting detection reagent • • for 6000 cm2 45-001-160 Chemiluminescent detection of Western blotting using film or CCD camera,

mid pg detection levelECF Western blotting reagent pack mouse • For 2500 cm2 45-000-943 Alkaline phosphatase conjugated secondary antibodies are used for the detection

of chemifluorescent proteinsECF Western blotting reagent pack rabbit • For 2500 cm2 45-000-945 Alkaline phosphatase conjugated secondary antibodies are used for the detection

of chemifluorescent proteins immobilized on membranesAmersham ECL Select™ • • For 1000 cm2 45-000-999 Chemiluminescent detection of Western blotting using film or CCD camera,

low pg detection level

Detection with filmAmersham Hyperfilm™ ECL (5 × 7 inches) • 50 sheets 45-001-504 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (18 × 24 cm) • 50 sheets 45-001-505 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (8 × 10 inches) • 50 sheets 45-001-507 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (24 × 30 cm) • 50 sheets 45-001-509 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (18 × 24 cm) • 100 sheets 45-002-808 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (5 × 7 inches) • 100 sheets 45-002-806 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (8 × 10 inches) • 100 sheets 45-002-807 Suitable for use with all blue and green light chemiluminescent system, such as

Amersham ECL and Amersham ECL Prime

Chemiluminesc

ence/F

ilm

Chemiluminesc

ence/C

CD

Fluoresc

ence

Many types of products are available in other formats and pack sizes. Please visit www.fishersci.com for more information.

BN0524125 05/2012

ECF Amersham ECL Amersham ECL Prime Amersham ECL Select Amersham ECL Plex

Detection technique Chemifluorescence Chemiluminescence Chemiluminescence Chemiluminescence Fluorescence

Limit of detection Mid picograms 20-100 pg Mid picograms 10-100 pg Low picograms < 10 pg Low picograms < 5 pg Low picograms 1-50 pg

Marker Rainbow marker DualVue markersRainbow markers

DualVue markersRainbow markers

DualVue Rainbow marker ECL Plex Fluorescence Rainbow marker

Blotting membrane Hybond-P Hybond ECL

Hybond ECLHybond-P

Hybond-PHybond ECL

Hybond-P Hybond ECL

Hybond-LFPHybond ECL

Primary antibody dilution 1:100 – 1:5000 1:100 – 1:5000 1:1 000 – 1:50 000 1:5000 – 1:30 000 1:100 – 1:5000

Secondary antibody dilution 1:2500 – 1:10 000 1:1 000 – 1:15 000 1:50 000 – 1:250 000 1:100000 – 1:300 000 1:1 250 – 1:4 000

Dynamic range of detection High to medium Medium to very low high to medium High to low High to very low

Fisher Scientific Cat. No. — 45-000-875 45-002-401 45-000-999 —

GE, imagination at work, and GE monogram are trademarks of General Electric Company.

© 2012 General Electric Company—All rights reserved. First published Jan. 2012.