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  • Agilent Technologies

    Agilent 240 GC/MS

  • Agilent Technologies, Inc. 2011

    Agilent Technologies, Inc.

    G3931-96002

    1 2011 5

    Printed in USA

    Agilent Technologies, Inc.5301 Stevens Creek Boulevard Santa Clara, CA 95051 USA

    Agilent

    Agilent

    Agilent

    /

    DFAR 252.227-70141995 6

    FAR 2.101a FAR 52.227-191987 6

    Agilent Technologies

    FAR 52.227-19 (c) (1-2)1987 6

    FAR 52.227-141987 6 DFAR 252.227-7015 (b) (2)1995 11 Limited

    Rights

  • 240 GC/MS 3

    1

    6

    8

    8 8Ion Preparation 8

    9

    EI 10

    10Ion Preparation 11 11 12

    CI 13

    13 CI 14Ion Preparation 15 15 15 15EI PCI 16

    CI 17

    17 19 19

    22Status and control 22

    23 23 25 26

    2

    28

  • 4 240 GC/MS

    28 29 30 31 32

    3

    36

    39240 GC/MS 39 39 40 48

    4

    53 53 54

  • Agilent 240 GC/MS

    Agilent Technologies

    1

    6

    8

    EI 10

    CI 13

    CI 17

    22

  • 6 240 GC/MS

    1

    240 GC/MS 3 1 EIPCI EI PCI

    NCI

    Ion Preparation

    SIS

    Automatic Methods Development (AMD)

    MS/MS

    MSn

    Multiple Reaction MonitoringMRM

    240 GC/MS

    240 GC/MS 7 1

  • 1

    240 GC/MS 7

    1

    E.M.

    EM

    H.E.D.

  • 8 240 GC/MS

    1

    EI

    PCI -

    MS

    Ion Preparation

    Ion Preparation

    MS/MSSISIon Preparation

  • 1

    240 GC/MS 9

    RF

    EM 240

  • 10 240 GC/MS

    1

    EI

    240 MS

    EI Ion Preparation

    EI

    A A+*

    fi+

    AGC

    AGC 6

    A + e- A+* + 2e- A+* He

    A+* A+

    A+* f+1 + n1 f+2 + n2 f+3 + n3

  • 1

    240 GC/MS 11

    Ion Preparation

    240 MS

    SISMS/MSSIS MS/MS

    Ion Preparation SISMS/MSMSn Multiple Reaction Monitoring

    MRMSIS 240 MS MS/MSMSn MRM MS/MS

    Ion Preparation EM RF -10 kV EM EM 105 m/z MS m/z -

  • 12 240 GC/MS

    1

    EI 2

    1

    AGC 6

    DFTPP BFB EPA

    MS

    NIST Wiley MS PMW 240 GC/MS

  • 1

    240 GC/MS 13

    CI

    CIEI2

    1 CI EI

    2 -

    CI EI CI EI CI EI

    CI

    2

    CH+5 C2CH

    +5

    (CH4 )+ + CH4 CH

    +5 + CH

    3

    CH+3 + CH4 C2CH+5 + H2

    CH4 + e- (CH4 )

    + + 2e-

    CH4 + e- CH+3 + e

    -+ H-

  • 14 240 GC/MS

    1

    CI

    2 4

    (A) Proton transfer (RH)+ + M (MH)+ + R

    (B) Hydride abstraction R+ + M (M H)+ + RH

    (C) Association R+ +z M (MR)+

    (D) Charge transfer R+ + M M+ + R

    R+ M

    CI ABM+1M-1 EI

    CI

    130 kcal/mol 200 kcal/mol CI 180.3 kcal/mol 1862 kcal/mol

    C

    240MSM1)M+29M+41)

    DEI

  • 1

    240 GC/MS 15

    Ion Preparation

    Ion Preparation 240 MS RF SISMS/MS

    Ion Preparation EM RF

    -10000VEM

    EM 105 m/z MS m/z - PCI

    240 GC/MS GC/MS CI CI

    PCI CI EI PCI

    Ion Preparation

  • 16 240 GC/MS

    1

    EI PCI

    EI CI -FAMEEI FAME CI M+1

    240 MS EI CI

    EI CI CI 60 CI CI 20

  • 1

    240 GC/MS 17

    CI

    CI CI

    1 Manual ControlChecks and Adjustments

    2 CI Gas AdjustmentParameters CI

    3 Start

    4 CI

    5 CI

    1

    2 CI 67

    3 CI Gas AdjustmentCI

    4 CI

    5 CI Gas AdjustmentM M+1 CI

  • 18 240 GC/MS

    1

    3 240 CH4 PCICH3CN PCICH3OH PCI

    20 A m/z 41 m/z 42 50%

    2

    3

  • 1

    240 GC/MS 19

    1 4 mL/min

    2 240 Ion Trap GC/MS

    RF

    EI CI CI

    EM

    EM 105 EM

  • 20 240 GC/MS

    1

    200 300 A 4 1

    Electron Lens TuningTurn on CI gas flow during tune CI / CI CI CI Manual Control CI

    3 123m/z 131 414 2

    RF

    RF

    PFTBA m/z 69131264414464 614

    2

  • 1

    240 GC/MS 21

    MS/MS SIS Ion Preparation

    DC

    DC m/z 414

  • 22 240 GC/MS

    1

    Start AcquisitionMS (Acquisitio)

    GC Not ReadyGC Not ReadyReady Windows GC

    23

    25

    Status and control

    Status and Control

    Run Time 0.00

    End Time

    ReadyNo Fault

    Start Acquisition

    4000.x.sms

    Edit MethodMethod Builder System Control

  • 1

    240 GC/MS 23

    MS GC Windows GC GC

    1 File

    2 Activate Method

    3

    Recent Files 8

    Open

    1 InjectInject Single Sample

  • 24 240 GC/MS

    1

    2 Inject Single Sample

    a

    b

    c

    d Defaults

    e Data Files

    f Inject

    MS

    System ControlWaiting for Injection of SampleSystem ControlWaiting

  • 1

    240 GC/MS 25

    Automation File EditorSystem Control (SampleList)

    System Control

    1 FileNew SampleListOpen SampleList

    2 SampleList

    Add

    3 Begin

  • 26 240 GC/MS

    1

    Status and Control

    System ControlMS Data Review

    240 GC/MS

  • Agilent 240 GC/MS

    Agilent Technologies

    2

    28

    28

    29

    30

    31

    32

  • 28 240 GC/MS

    2

    10 20

    1

    2

    3

    System ControlStartup/Shutdown

    MS 1

    1

    100%

    200 300 mAmps

    9 13

    < 20 Torr

    < 50 mTorr

  • 2

    240 GC/MS 29

    100% 100% 240 Ion Trap GC/MS Service.mth Agilent 240 Ion Trap GC/MS

    MonitoringEM

  • 30 240 GC/MS

    2

    Diagnostics Tests240 GC/MS 240

    80 220 PAHghia,h1,2,3-cd

    1 2

    GC MS 20

    50

    240 GC/MS System ControlTemperaturesBakeout

    MS

  • 2

    240 GC/MS 31

    230

    110

    280

    12

    System ControlStartup/ShutdownOperating ConditionsTurbo Pump Speed 100% Startup/ShutdownOperating Conditions

    100%

    Agilent 240 Ion Trap GC/MS

  • 32 240 GC/MS

    2

    240 GC/MS Startup/Shutdown 35%

    80 OFF 5

    Start Up

    RF

    Manual ControlChecks and Adjustments RF

    MS

    MS

  • 2

    240 GC/MS 33

    RF

    1 Manual ControlChecks and Adjustments>RF Ramp Adjustment

    2 Start

    3 240 GC/MS RF Adjustment ResultsOK

  • 34 240 GC/MS

    2

    1 PFTBA FC-43

    2 Manual ControlChecks and Adjustments>Cal Gas Adjustment

    3 240 GC/MS

    Adjustment ResultsOK

    /

    /

    /105 EM EM / EM

  • Agilent 240 GC/MS

    Agilent Technologies

    3

    36

    39

    240 GC/MS 39

    39

    40

    CI 44

    48

    MSMethod Builder

    MS 4 240 GC/MS 4

    4

    RF

    RF

    RF

    AGC

  • 36 240 GC/MS

    3

    1 WorkstationMethod Builder

    2 Create a New Method File

    Do not display this dialog at startup

    3 Instrument 1Next PC

  • 3

    240 GC/MS 37

    4 Next

    5 Next

    6 Finish

    240

  • 38 240 GC/MS

    3

  • 3

    240 GC/MS 39

    1 File

    2

    3

    4 Save

    240 GC/MS

    MS 240 GC/MS 1 EI CI

    A/D

    10

    m/z 10

  • 40 240 GC/MS

    3

    240 GC/MS EICIEI/MS/MS CI/MS/MS

    650 250 /

    MS/MS

    CI 240

    MS EI CIMS/MSMultiple Reaction MonitoringMRM

    SIS 2

  • 3

    240 GC/MS 41

    240 GC/MS 3 Normal

    Normal: Automatic Gain Control 5000 /sec

    Fast: Automatic Gain Control 10000 /sec

    Fastest: 10000 /sec Full

    [General parameters]

    Scan TimeScans Averaged Data Rate

    Scan Parameters 3 3

    2

    Normal Fast Fastest

    501000 1 3 0.76 sec 0.47 sec 0.41 sec

    501000 4 3 1.08 sec 0.79 sec N/A

    50400 DFTPP 3 0.70 sec 0.59 sec N/A

  • 42 240 GC/MS

    3

    Mass Defect

    NIST MS

    Mass Defect Mass Defect C2Br6 497.51002 497 498

    Multiplier Offset Manual ControlModule Attributes EM 105 300V EM EM MS/MS

    Count Threshold 1 2 3

    Customize

    mScan Time

    uScans AveragedMaximum Scan Time

    Data Rate

  • 3

    240 GC/MS 43

    Ionization control

    TICAGC

    Target TIC 10,000 MS

    20,000 40,000 Target TIC

    EI

    Emission Current

    EI/MS/MS MS/MS

  • 44 240 GC/MS

    3

    CI

    CI CI Reaction Storage LevelEjection Amplitude (V)Max Reaction Time)Save

    Reagent Low Mass

    Reagent High Mass EI

    Reagent Low MassReagent High MassReaction Storage Level CI 294119

    CH4+

    CH4 C2H5+

    Reaction Storage Level Reaction Storage Level CI Reaction Storage Level 352 m/z RF 25 m/z 19 m/z CI Storage Level Ejection Amplitude

    Ejection AmplitudeV Reagent High Mass

    CI Storage Values Ejection Voltages CI

    Max Reaction Time

    1 2000 100

  • 3

    240 GC/MS 45

    240

    CI CI

    Scan parameters

    MS MS/MS240 GC/MS

  • 46 240 GC/MS

    3

    GC/MS Full ScanMass Range Low MassHigh Mass 240 GC/MS AGC

    TuneAu

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