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  • Agilent Technologies

    Agilent 1220 Infinity LC

    User Manual

  • Notices © Agilent Technologies, Inc. 2010-2014, 2015

    No part of this manual may be reproduced in any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

    Manual Part Number G4280-90016 Rev. D

    Edition 02/2015

    Printed in Germany

    Agilent Technologies Hewlett-Packard-Strasse 8

    Warranty

    The material contained in this docu- ment is provided “as is,” and is sub- ject to being changed, without notice, in future editions. Further, to the max- imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par- ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor- mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep- arate agreement shall control.

    Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor- dance with the terms of such license.

    Restricted Rights Legend If software is for use in the performance of a U.S. Government prime contract or subcon- tract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR 2.101(a) or as “Restricted computer soft- ware” as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will

    receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

    Safety Notices

    CAUTION

    A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi- tions are fully understood and met.

    WARNING

    A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi- cated conditions are fully under- stood and met.

    76337 Waldbronn

    1220 Infinity LC

  • In This Book

    In This Book

    This manual covers the Agilent 1220 Infinity LC System configurations:

    • G4286B

    • G4288B/C

    • G4290B/C

    • G4294B

    1 Introduction

    This chapter provides an overview of the Agilent 1220 Infinity LC available configurations.

    2 Site Requirements and Specifications

    This chapter provides information on environmental requirements, physical and performance specifications.

    3 Installation

    This chapter provides an overview on shipment content and installation.

    4 LAN Configuration

    This chapter provides information on connecting the instrument to the Agilent ChemStation PC.

    5 Solvent Delivery System Description

    This chapter provides an overview on the operational principles of the solvent delivery system (pump and optional degasser).

    6 Injection System Description

    This chapter provides an overview of the operational principles of the injection systems: manual injector and autosampler.

    1220 Infinity LC 3

  • In This Book

    7 Column Oven Description

    This chapter provides an overview of the operational principles of the column oven.

    8 Detector Description

    This chapter provides an overview of the operational principles of the detector.

    9 Test Functions and Calibration

    This chapter describes the tests, calibrations and tools that are available with the Instrument Utilities software or the Lab Advisor.

    10 Error Information

    This chapter provides information on the error messages that might be displayed, and gives the possible causes and suggestions on their solutions.

    11 Maintenance

    This chapter provides general information on maintenance of the instrument.

    12 Parts for Maintenance

    This chapter provides information on parts for maintenance.

    13 Upgrading the Agilent 1220 Infinity LC

    This chapter provides information for upgrading the LC system.

    14 Identifying Cables

    This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.

    15 Appendix

    This chapter provides addition information on safety, legal and web.

    4 1220 Infinity LC

  • Contents

    Contents

    1 Introduction 9

    Agilent 1220 Infinity LC Configurations 10 Agilent 1220 Infinity LC VL Configurations 11 Early Maintenance Feedback 12

    2 Site Requirements and Specifications 15

    Site Requirements 16 Physical Specifications 19 Performance Specifications 20

    3 Installation 29

    Unpacking Your System 30 Installing the Hardware 34 Connecting and Configuring the Instrument to the Chromatographic Data System 43 Connecting the Agilent 1220 Infinity LC to the PC 44 The Instrument Utility / LabAdvisor Software 46 Configuration of the Instrument After an Upgrade Installation 47 Priming the System and Performing the ‘Installation Check’ 48 Performing a ‘Checkout Run’ 49

    4 LAN Configuration 51

    To do first 52 TCP/IP parameter configuration 54 Configuration Switches 55 Initialization mode selection 56 Dynamic Host Configuration Protocol (DHCP) 60 Link configuration selection 63 Automatic Configuration with BootP 64 Storing the settings permanently with Bootp 74 Manual Configuration 75

    1220 Infinity LC 5

  • Contents

    5 Solvent Delivery System Description 81

    Overview 82 Degasser 83 Principles of Operation 84 Compressibility Compensation 88 Variable Stroke Volume 90 Using the Pump 91

    6 Injection System Description 93

    Manual Injector 94 Autosampler 97

    7 Column Oven Description 109

    Column Oven 110

    8 Detector Description 111

    Detector Types 112 Agilent 1220 Infinity LC Variable Wavelength Detector (VWD) 113 Agilent 1220 Infinity LC Diode Array Detector (DAD) 114 Match the Flow Cell to the Column 130

    9 Test Functions and Calibration 135

    Agilent 1220 Infinity LC System 137 Solvent Delivery System 139 Autosampler 149 Column Oven 155 Variable Wavelength Detector (VWD) 157 Diode Array Detector (DAD) 167

    6 1220 Infinity LC

  • Contents

    10 Error Information 191

    What are Error Messages? 194 General Error Messages 195 Pump Error Messages 201 Autosampler Error Messages 213 General Detector Error Messages 222 VWD Detector Error Messages 225 DAD Detector Error Messages 230

    11 Maintenance 237

    PM Scope of Work and Checklist 239 Cautions and Warnings 240 Solvent Delivery System 242 Manual Injector 263 Autosampler 267 Variable Wavelength Detector (VWD) 285 Diode Array Detector (DAD) 295 Algae Growth in HPLC Systems 316 Replacing the Module’s Firmware 318

    12 Parts for Maintenance 319

    1220 Infinity LC System 320 Solvent Delivery System 322 Injection System 331 Column Oven 339 Detector 340

    13 Upgrading the Agilent 1220 Infinity LC 347

    Oven Upgrade 348

    1220 Infinity LC 7

  • Contents

    14 Identifying Cables 349

    Cable Overview 350 Analog Cables 352 Remote Cables 354 BCD Cables 357 CAN/LAN Cables 359 Agilent 1200 module to PC 360

    15 Appendix 361

    General Safety Information 362 Solvent Information 365 Radio Interference 367 UV Radiation 368 Sound Emission 369 Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 370 Declaration of Conformity for HOX2 Filter 371 Agilent Technologies on Internet 372

    8 1220 Infinity LC

  • 1220 Infinity LC

    1 Introduction

    Agilent 1220 Infinity LC Configurations 10

    Agilent 1220 Infinity LC VL Configurations 11

    Early Maintenance Feedback 12

    EMF counters for the pump 12

    EMF counters for the autosampler 13

    EMF counters for the variable wavelength detector 14

    EMF counters for the diode array detector 14

    This chapter provides an overview of the Agilent 1220 Infinity LC available configurations.

    9Agilent Technologies

  • 1 Introduction Agilent 1220 Infinity LC Configurations

    Agilent 1220 Infinity LC Configurations

    Available configurations of Agilent 1220 Infinity LC

    The Agilent 1220 Infinity LC is available in four different configurations. Possible components include isocratic pump, dual- channel gradient pump (with degasser), manual injector

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