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Agilent Technologies

Agilent 1200 Series Isocratic Pump

User Manual1200 Series Isocratic Pump User Manual

1200 Series Isocratic Pump User Manual

Notices Agilent Technologies, Inc. 2006-2007, 2008

No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part NumberG1310-90011

Edition11/08

Printed in Germany

Agilent TechnologiesHewlett-Packard-Strasse 8 76337 Waldbronn

Research Use Only

Not for use in Diagnostic Procedures.

Warranty

The material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor-mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall control.

Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor-dance with the terms of such license.

Restricted Rights LegendIf software is for use in the performance of a U.S. Government prime contract or subcon-tract, Software is delivered and licensed as Commercial computer software as defined in DFAR 252.227-7014 (June 1995), or as a commercial item as defined in FAR 2.101(a) or as Restricted computer soft-ware as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis-closure of Software is subject to Agilent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will

receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi-tions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi-cated conditions are fully under-stood and met.

1200 Series Isocratic Pump User Manual 3

Contents

Contents

1 Introduction 5

Introduction to the Isocratic Pump 6Overview of the Hydraulic Path 7Instrument Layout 13Electrical Connections 14Agilent 1200 Series Interfaces 16

2 Site Requirements and Specifications 17

Site Requirements 18Physical Specifications 21Performance Specifications 22

3 Installing the Pump 25

Unpacking the Isocratic Pump 26Optimizing the Stack Configuration 28Installing the Isocratic Pump 31Connecting Modules and Control Software 34Flow Connections of the Isocratic Pump 37Priming and Purging the System 40

4 Using the Isocratic Pump 45

Hints for Successful Use of the Pump 46Solvent Information 47Prevent Blocking of Solvent Filters 48Algae Growth in HPLC Systems 49

5 Optimiziming Performance 51

When to Use a Vacuum Degasser 52When to use the Seal Wash Option 53When to Use Alternative Seals 54Optimize the Compressibility Compensation Setting 55

4 1200 Series Isocratic Pump User Manual

Contents

6 Troubleshooting and Diagnostics 57

Agilent Lab Advisor Software 58Overview of the Pumps Indicators and Test Functions 59Status Indicators 60User Interfaces 62

7 Maintenance 63

Introduction to Maintenance and Repair 64Early Maintenance Feedback (EMF) 67Overview of Maintenance and Repair 69Simple Repairs 71

8 Parts and Materials for Maintenance 99

Overview of Main Assemblies 100Pump Head Assembly 104Pump Head Assembly with Seal Wash Option 106Outlet Ball Valve Assembly 108Purge Valve Assembly 109Active Inlet Valve Assembly 110Accessory Kit G1311-68705 111Seal Wash Option Kit G1311-68711 112

9 Appendix 113

General Safety Information 114The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 118Lithium Batteries Information 119Radio Interference 120Sound Emission 121Solvent Information 122Agilent Technologies on Internet 124

5

1200 Series Isocratic Pump User Manual

Agilent Technologies

1Introduction

Introduction to the Isocratic Pump 6

Overview of the Hydraulic Path 7

How Does the Pump Work? 8

How Does Compressibility Compensation Work? 11

How Does Variable Stroke Volume Work? 12

Early Maintenance Feedback (EMF) 12

Instrument Layout 13

Electrical Connections 14

Agilent 1200 Series Interfaces 16

6 1200 Series Isocratic Pump User Manual

1 IntroductionIntroduction to the Isocratic Pump

Introduction to the Isocratic Pump

The isocratic pump comprises a pump assembly and a damping unit. Degassing is not included but avacuum degasser is available as a separate product for applications that require best flow stability especially at low flow rates or highest detector sensitivity. This is most likely required to run small internal diameter columns (2 mm and 1 mm i.d.) which require low flow rates. A solvent cabinet provides enough space for up to four one liter bottles. An active seal wash (optional) is available when the pump is used with concentrated buffer solutions.

Figure 1 Overview of the Isocratic Pump

1200 Series Isocratic Pump User Manual 7

Introduction 1Overview of the Hydraulic Path

Overview of the Hydraulic Path

The isocratic pump is based on a two-channel, dual-plunger in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 400 bar.

The pump assembly includes a pump head with an active inlet valve which has a replaceable cartridge, and an outlet valve. A damping unit is connected between the two plunger chambers. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head.

An active seal wash (optional) is available when the isocratic pump is used with concentrated buffer solutions.

Figure 2 Hydraulic Path

8 1200 Series Isocratic Pump User Manual

1 IntroductionOverview of the Hydraulic Path

How Does the Pump Work?

The liquid runs from the solvent reservoir to the active inlet valve. The pump assembly comprises two substantially identical plunger pump units. Both pump units comprise a ball-screw drive and a pump head with one sapphire plunger for reciprocating movement in it.

A servo-controlled variable reluctance motor drives the two ball screw drives in opposite directions. The gears for the ball-screw drives have different circumferences (ratio 2:1) allowing the first plunger to move at twice the speed of the second plunger. The solvent enters the pump heads close to the bottom limit and leaves it at its top. The outer diameter of the plunger is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between. The first plunger has a stroke volume in the range of 20 100 l depending on the flow rate. The microprocessor controls all flow rates in a range of 1 l 10 ml/min. The inlet of the first pumping unit is connected to the active inlet valve which is processor-controlled opened or closed allowing solvent to be drawn into the first plunger pump unit.

The outlet of the first plunger pump unit is connected through the outlet ball valve and the damping unit to the inlet of the second plunger pump unit. The outlet of the purge valve assembly is then connected to the following chromatographic system.

1200 Series Isocratic Pump User Manual 9

Introduction 1Overview of the Hydraulic Path

Figure 3 Principle of the Isocratic Pump

When turned on, the isocratic pump runs through an initialization procedure to determine the upper dead center of the first plunger. The first plunger moves slowly upwards into the mechanical stop of the pump head and from there it moves back a predetermined path length. The controller stores this plunger position in memory. After this initialization the isocratic pump starts operation with the set parameters. The active inlet valve is opened and the down-moving plunger draws solvent into the first pump head. At the same

10 1200 Series Isocratic Pump User Manual

1 IntroductionOverview of the Hydraulic Path

time the second plunger is moving upwards delivering into the system. After a controller-defined stroke length (depending on the flow rate) the drive

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