agilent 1200 hplc 现场培训

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Agilent 1200 HPLC 现场培训

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  • Agilent 1200 LC

    B02.01C

  • 1200LC

    ,,

    1 Agilent 1200LC

    G1310A :()G1312AG1312B XL()G1311A() G1329A G1316A,G1316B XL G1314B,G1314C XLVWD G1362A G1315B ,G1315C XL (DAD) G1365B ,G1365C XL (MWD) G1321A (FLD) : Zorbax Eclipse XDB-C18 150 x 4.6 mm, 5um column

    P/N 993967-902 /5063-6600

    2

  • :

    ()

    1,Windows XPIP Bootp Service 2 1200 LC

    3 1

    Agilent Chemstation, 1

    1200LC

    4, ,

    5

    6

    7

    8 3-5ml/min

    9

    ,

    10

    111.0ml/min

    12

  • 1

  • 2

    This is for test!

    3

    , 1.5ml/min10 min B

    70.0,(A=100-B-C-D) , ,

  • 4:G1329A

    , 1.0ul ,6

    ----

    ----

    ---

    5:

    G1316A : ,40,

    >> ,,

  • G1316B: ,,

    >> ,,

    6VWD:

    G1314B: ,254nm,

    (),, >0.1min (2s)

    G1314B:13.74HZ

    1min

    =300nm

  • G1314C: ,254nm, (

    ),, >0.1min (2s)G1314C:

    55HZ

    1min

    =300nm

    7DAD:

    G1315B: : 254nm,=4nm, =360nm,=100nm;

  • 100nm

    -

    G1315C XL:;8

    8 RID:

  • :

    : 20ul ; 1.5ml/min; : 35 ;

    25 ;: ;() : 4s

    : ---2,Off,

    ,5,

    ()---4S

    ---

    ***** RIDRID

  • 9 FLD:

    P/N 01018-68704 1:10

    ODS Hypersial column 125mmx4.0mmIDX5u.

    A:Water 35% B: 65%

    5ul; : 30 ; =246nm,

    =317nm ;PMT=10

    =4s. 4min

    :200-700nm,1nm,

    : 280-900nm, 1nm,

    PMT:10,, PMT

    4s

  • 10

    11

    12 , 1, ,,

    (, 10, 2)

  • 13

    /

    : --,

    /----

    vwd 0001vwd

    0002

    14 , 15

    (

    )

    DAD, VWD

  • :

    1

    2

    3

  • 4:

    5:

  • ():

    shut down

    Agilent 1200

    ()Agilent 1200 LC

    /

    *

    *seal-wash 120090%+10%seal-wash

    ****

    3

    4

  • 1 ?

    2 HPLCSeal-wash HPLC,,

    ,,,,

    ,Seal-wash

    0.1mol0.1mol,.

    : 90%+10%.

    3 Agilent 1200LC? HPLC,

    4~5

    1-2

    Agilent 1200LC

    0.17mm ------ ;0.12mm-------

    PEEK -----0.13mm;0.18mm;0.25mm;0.5mm

    : .

    ***Agilent 1/16in , ,

    ,

    4 O2

  • FLD

    10-

    15min

    Agilent1200LC

    5

    PH=4-7

    A

    B

    C0.0001---0.001M.

    D:,

    E

    6 Agilent 1200LC Agilent 1200LC

    A

    BSeal-wash

    C

    DPurge ValvePurge Valve10bar

    E

  • 7

    p/n0905-1420 2/pk

    ***0-200bar

    200bar

    8

    A

    /D

    A

    AD

    9 ? HPLC,

    4~5

    1-2

    ,Agilent 1200LC ,

    ,

    PEEK