Agilent 1100 Series LC/MSD

New standards for valueand performance

Analyze a wide variety of samples

Standard positive and negative ionizationmodes, and a choice of electrospray ion-ization (ESI) or atmospheric-pressurechemical ionization (APCI), allow you toanalyze a wide variety of compoundssuch as:

Drugs and drug metabolites

Proteins and peptides

Pesticides and herbicides

The advantages of mass spectral data—made easy and reliable

These results from the analysis of a sulfadrug mixture show the complementary combination of mass chromatograms, UVchromatograms, and spectral information.

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The Agilent 1100 Series LC/MSD offers you the sensitivity, selectivity,and depth of molecular information inherent in mass spectrometry—ina package that is rugged, reliable, and surprisingly easy to learn, operate,and maintain.

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Increase confidence in results andshorten analysis times

The LC/MSD provides molecular weightand structural information that comple-ments the spectral information from adiode-array UV-Visible detector. Theseorthogonal sets of data provide confidentcompound identification from a singlechromatographic run—in one report.

You do not need to derivatize analyteslacking chromophores to obtain data

Mass spectral data make it easy to detectand identify low-level impurities

The ability to quantitate using unique masses means quantitative performance is less affected by matrixinterference and unresolved chromatographic peaks

Fully-resolved peaks are not required formass spectral analysis; allowing you toshorten chromatographic runs andreduce sample preparation

Reproducible in-source fragmentationcan confirm sample identity

Use existing HPLC methods

With its patented orthogonal-spray ionsource and high-capacity drying gas system, the LC/MSD provides excellentsensitivity and reproducibility over a widerange of LC operating conditions. Thisminimizes the need to alter establishedLC methods for LC/MS analyses.

Flow Rates

Up to 1.0 ml/min ESIUp to 1.5 ml/min APCI

Solvent Composition

100% aqueous to 100% organic

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Save time with fast, easy system setup

Automated tuning and a built-in calibrantdelivery system make system setup fastand easy, while ensuring the highest sensitivity and reproducibility.

Calibrant delivery is automatic, without replumbing

Pre-made calibrants save time and avoid mistakes

Easy-to-use software allows you to man-ually tune the instrument when desired

Autotune and automated calibrant deliveryshorten system setup time and ensure the high-est sensitivity and day-to-day reproducibility.

Automated switching improves performance

The LC/MSD’s automated switching valvesimplifies plumbing and provides a singleLC connection. This reduces leakage,minimizes extra-column band broadening,and allows you to divert the void volumeas part of a method.

An optional, integrated manual injectionvalve gives you a way to quickly introducesamples that do not require separation.

Switch from ESI to APCI in just a few minutes

A hinged, swing-out spray chamberspeeds switching between modes andmakes cleaning easier.

Improve uptime with easy maintenance

The LC/MSD is designed to minimizemaintenance requirements and makewhat maintenance is required fast andeasy.

Orthogonal spray orientation keeps thecapillary and ion optics cleaner

Large sampling orifice prevents capillaryplugging

Slide-out, self-aligning components suchas the sprayer make maintenance easier

Diagnostic software can log the hours ofsystem use and prompt you when keycomponents are due for servicing

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Analyses at low picogram levels are common thanks to Agilent’spatented orthogonal-spray ion source design and high-capacity dryinggas system. Data are exceptionally reproducible, with RSDs of lessthan 5 percent. For quantitative performance, response is linear overseveral orders of magnitude.

Gain the advantages of orthogonal spray...

Agilent revolutionized electrospray technology, replacing traditional in-linesource geometry with a fixed-positionsprayer orthogonal to the mass analyzer’soptical axis. This approach:

Greatly reduces background from solventclusters and mobile-phase adducts

Lets you optimize both desolvation andion transport for improved sensitivity

Substantially reduces ion source contamination and the need for cleaning

Eliminates the need to adjust the positionof the sprayer—even when the flow rateor solvent composition changes

Achieve the highest sensitivity and reproducibility

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HPLC inlet

Skimmer

Capillary

Octopole

Quadrupole

Lenses

Fragmentationzone (CID)

Sprayer

Orthogonal spray orientationand high-capacity drying gassystem improve instrumentperformance and decreasemaintenance requirements.

Orthogonal-spray ion source isvery tolerant of non-volatile com-ponents in samples or mobilephase. This ion source was stillgenerating useful data.

Swing-out spray chambermakes maintenance easier.

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3 Torr

1 x 10-5 Torr

...excellent sensitivity...

The LC/MSD delivers excellent sensitivityin all operating modes. And the extraspecificity provided by mass spectraldata makes that sensitivity more useful.

When scanning a range of masses,LC/MSD sensitivity is comparable to thesensitivity of a good diode array UV detector

Sensitivity is enhanced 5 to 100 timeswhen the LC/MSD is set to monitor onlyselected masses characteristic of thecompounds being analyzed (selected ionmonitoring mode)

With its improved ion optics, sensitivity ofthe LC/MSD SL model is 10 times greaterthan the sensitivity of previous LC/MSDs

Five replicate injections of 1 picogram of reserpine on column inESI mode (SL model).

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1 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103

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Injection #

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ESI

APCI

Caffeine reproducibility in ESI and APCI modes of operation

Standard Deviation = 0.01Average= 0.953

Standard Deviation =0.008Average = 0.945

Reproducible dataincrease confidence in methods and results.

Added sensitivity extends linear range to improve quantitation results.

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...and enhanced stability and reproducibility

Good reproducibility yields better qualita-tive and quantitative results. The LC/MSDexhibits exceptional stability and repro-ducibility, giving you more confidence inthe validity of your methods and data.

The VL model can provide linear quanti-tation results over three orders ofmagnitude. The SL model is even better:it can provide linear quantitation resultsover four orders of magnitude.

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Simplify operation

The Microsoft® Windows® -based userinterface makes the software easy tolearn and operate.

System control and data acquisitionparameters require only two windows

Some parameters are calculated auto-matically. For example, if you provide thechromatographic peak width, the systemautomatically optimizes the data acquisition rate

Other parameters are preset or reliabledefaults are provided

Online help includes tutorials to lead youthrough tasks. Digital videos show you howto perform common maintenance tasks

Acquire more data quickly and easily

The software is fully inte-grated with the Agilent LC3D ChemStation software.

In this example, the FIA series table was used to make repeatedflow injections of a caffeine standard while the fragmentor voltagewas varied.

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The LC/MSD ChemStation software is fully integrated with our LC 3DChemStation software. Thanks to an easy-to-use graphical user inter-face and powerful automation features, both new and experiencedusers can quickly take advantage of the LC/MSD’s capabilities.

Speed analyses

Automated flow-injection analysis (FIA)allows you to quickly analyze samplesthat do not require separation. When youare developing methods, FIA can help youfind the best settings for MS parameters.Setup is easy using the FIA series table.

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Maximize the information from each analysis

The multisignal analysis found in theLC/MSD SL model gives you the power toacquire up to four separate signals, eachusing a different acquisition mode, from asingle sample injection. Choose positive ornegative ionization, selected ion monitoring(SIM) or scanning, and fragmentor voltage.

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Maximize the information from asingle analysis by using up tofour different acquisition modes.

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Acquiring positive and negative ionshelps you determine which mode pro-duces the best response for a particularcompound. And seeing both [M+H]+ and[M-H]- ions can confirm molecular weight

Combining SIM and scan data meansthat, from the same run, you get theutmost sensitivity for quantitation of low-level target compounds and completespectra for other high-level compoundsand unknowns

Combine the power of LAN and the Intranet

The Agilent ChemStation software is compatible with the latest in networkingtechnologies.

Instruments can be controlled, and theiroperating status checked, from any PC—even if it is not in the laboratory

Complete web-page-ready reports canbe sent directly to your desktop

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Acquiring data with low and high frag-mentor voltages can provide you withmolecular ion information to confirmmass and fragmentation information todetermine structure

And multisignal data acquired togethercan be linked for easier processing

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Speed peak identification

Automatic alignment of UV and mass-spectral data allows rapid visualizationand peak-to-peak data comparisons.Each peak in the MS and UV chro-matograms is integrated and labeled witha retention time. In mass spectra, majorions are automatically labeled with massinformation.

Speed up data processing for greater productivity

Peak purity software with a multi-component peak in red. The softwaredraws the extracted ion chromatograms below to identify components.

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Powerful, easy-to-use software speeds processing of qualitative andquantitative data. UV and mass-spectral data can be processedtogether using the same software tools. Data processing can be automated and included as part of a method.

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Identify coeluting compounds quickly

Peak purity software uses UV data ormass-spectral data or both to identifychromatographic peaks with incom-pletely-resolved compounds. This isuseful for developing separation methodsor for choosing quantitation ions withoutfurther refinement of the separation.

Toggle buttons make it possible to quicklymove from one impure peak to the nextacross a chromatogram

The peak purity report displays the reten-tion time, number of possible peakcomponents, and characteristic ions foreach peak in a chromatogram

Visualize complex data rapidly

Isoabundance plots of mass-spectraldata allow rapid visualization of complexdata. This feature is useful for detectingpatterns of specific functional groups.

Identify compounds fast

Optional NIST mass-spectral librarysearching quickly identifies compoundsby matching MS spectra with user-createdlibrary spectra. You can combine UV andmass-spectral searching and reporting.The software is optimized for both ESIand APCI data.

Quickly identify com-pounds with optionallibrary search software.

Batch review software helps you evaluate and, if necessary, reprocessthe results for large batches of samples.

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Speed quantitation through automation

Instrument control, data analysis, andreporting can be automated and includedas part of your method.

You can quantitate using both UV and MSsignals in a single calibration table

Quantitation methods can include peakarea or peak height, external standards(ESTD), internal standards (ISTD), peakratios, and multi-point calibration curves

Batch review software provides rapid,interactive user evaluation of results forlarge batches of samples. You can quicklyreview a set of calibration compounds,controls, and samples; and, only if neces-sary, manually reintegrate peaks andrecalculate the calibration curves andsample results

Automated report options are available.Or the drag-and-drop report builder pro-vides an easy way to customize reports

System-wide validation

Because the LC/MSD is a member of the Agilent 1100 Series family of LC andMS modules and systems, you get a com-plete system approach to installationqualification (IQ) and operational qualifi-cation/performance verification (OQ/PV).

The Declaration of System Validationqualifies the Agilent LC/MSD designaccording to documented procedures

IQ and OQ/PV software and services qualify system installation and operation.The ChemStation software includes onlinestandard operating procedures (SOPs) for performing OQ/PV, maintenance, and repairs

The Declaration of Conformity for per-formance verifies that the LC/MSD met specifications prior to its shipmentfrom Agilent

Increase uptime with validation tools, diagnostics, and support

OQ/PV softwarehelps assessongoing systemperformancequickly.

The Agilent 1100 Series LC/MSD includes an array of integrated valida-tion tools to help you meet the most demanding regulatoryrequirements. High reliability, easy-to-use diagnostics, and supportservices increase instrument uptime.

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Maximize uptime

Built-in diagnostics and remote accessmaximize uptime and make maintenanceeasier.

Early maintenance feedback (EMF) software tracks operating time and alerts you when components are due for maintenance

Built-in diagnostic software makes system troubleshooting easy

If maintenance is required, multimediatools, including digital videos, show youhow to do it

Remote access enables remote operationand troubleshooting

Enjoy a range of support services

Agilent offers a broad range of supportservices designed to improve instrumentuptime and to help you comply with regu-latory requirements.

Industry-specific service bundles aredesigned to meet the needs of pharma-ceutical, hydrocarbon processing,chemical, and environmental laboratories

ISO-registered training courses are avail-able at selected locations world wide.On-site courses can be tailored to meetspecial needs

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ChemStationSecurity Packsoftware supports21 CFR Part 11compliance

Software, test files, and methods help thesystem manager assess the functionalityof the system

For data integrity and traceability, thesoftware saves instrument conditions,together with raw data, in checksum-protected binary files

Two password-protected levels of systemaccess ensure operators have only theaccess they need

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Capillary electrophoresis with tandemUV and MS detection

The LC/MSD can be directly coupled to theAgilent 3D CE system to assist with theanalysis of complex mixtures. An optionalcapillary sprayer optimizes performancefor CE/MS analyses.

Combinatorial libraries solution

This molecular-weight confirmationsolution is for the rapid molecular-weight confirmation of combinatoriallibraries—from sample analysis toreporting. Using this system, you canrapidly identify the products of combinato-rial syntheses and estimate their purities.The products can then be purified asnecessary before screening.

Mass-based fraction collection lets youmonitor specific masses and their adductsto trigger fraction collection, thereby col-lecting the compounds of interest.

Complete analytical solutions

Agilent’s 1100 Series LC/MSD Trap system for MS/MS and MSn analyses.

Agilent’s capillary electrophoresis (CE) system with tandem UV and MS detection.

Agilent offers complete analytical solutions with fully-integratedhardware and software. All components are supplied and supported by Agilent.

Agilent’s molecular-weight confirmationsystem for combinatorial libraries.

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MS/MS and MSn solutions

The Agilent 1100 Series LC/MSD Trapsystem provides the multiple stages ofMS data required to solve challengingresearch questions faster and with moreconfidence.

Multiple stages of MS deliver the informa-tion needed to unambiguously characterizecompounds and elucidate structures.

MS/MS provides greater specificity andsensitivity and therefore faster, moreaccurate quantitation—even when chro-matographic peaks are not completelyresolved or sample matrices are complex.

Protein and peptide analysis solutions

Optional software tools make the analysisof proteins and peptides easier.

Protein and peptide analysis softwareeliminates the tedious manual calcula-tions required to match the masses ofpeptide digest fragments to those pre-dicted by digest conditions

Deconvolution software automaticallydeconvolutes mass-spectral data frommultiply-charged ions and assigns molec-ular weights to the mass peaks

Protein database search software searchesprotein databases against mass-spectralinformation generated by peptide maps

High-performance design features

Orthogonal-flow sprayer with pneumaticnebulizer – enhances sensitivity over awide range of LC conditions and reducesmaintenance requirements

High-capacity solvent drying system withuser control of gas flow rate and gastemperature – reduces chemical noiseand permits the analysis of thermally-sensitive compounds

Dielectric platinum-plated capillary – permits independent optimization ofspray chamber and ion optics

User-adjustable in-source fragmentation

Air-cooled vacuum system with dual tur-bomolecular pumps – eliminates theneed for cooling water

Fraction collection capability – can moni-tor and trigger the collection of fractionsbased on mass, an analog signal input, or time. For mass-based determination,up to 16 m/z values can be monitored.Requires optional I/O Accessory Module.

Specifications

Mass rangeVL – m/z 50 - 1500SL – m/z 50 - 3000

Mass accuracy± 0.13 u within the calibrated mass rangein scan mode

Mass axis stability± 0.13 u over 8 hours

SIM sensitivity

• API-electrospray at 400 µl/min or APCI at 1000 µl/min

• Selected ion monitoring of m/z 609.3

• Positive ionization

Quantity Signal-to-Noise Ratio

VL 10 pg 50:1 RMSreserpine (10:1 peak-to-peak)

SL 1 pg 50:1 RMSreserpine (10:1 peak-to-peak)

Scan sensitivity

• API-electrospray at 400 µl/min orAPCI at 1000 µl/min

• Scan range m/z 100 - 650

• Extracted ion at m/z 609.3

• Positive ionization

Quantity Signal-to-Noise Ratio

SL 50 pg 50:1 RMSreserpine (10:1 peak-to-peak)

Multiple signal acquisition (SL model only)Ability to cycle through four differentacquisition modes on a scan-by-scanbasis within a single run.

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For more information

For more information about the Agilent1100 Series LC/MSD, call toll free: 1-800-227-9770 (in the U.S.) or 1-800-341-2477 (in Canada).

In other countries, please call your localAgilent Technologies analytical salesoffice or authorized Agilent Technologiesdistributor.

You can also visit our site on the WorldWide Web at:

http://www.agilent.com/chem

The Agilent 1100 Series LC/MSD hasbeen designed and manufactured undera quality system that has been registeredto ISO 9001.

Microsoft® is a U.S. registered trademark ofMicrosoft Corporation

Windows® is a U.S. registered trademark ofMicrosoft Corporation

Information, descriptions, and specifications in thispublication are subject to change without notice.

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