a g c c g a t g t c g t c g t c t a c c a t g c a t

13
A G C C G A T G T C G T C G T C T A C C A T G C Polymerase Chain Reaction (PCR) Part I Segment of Interest

Upload: tamyra

Post on 26-Jan-2016

122 views

Category:

Documents


0 download

DESCRIPTION

Polymerase Chain Reaction (PCR). Part I. Segment of Interest. A G C C G A T G T C G T C G T C T A C C A T G C A T. Part II. C C G A T. A T G G T. Part III. C C G A T G T C G T. A G C A G A T G G T. Part IV. C C G A T G T C G T. A G C A G A T G G T. Part IV. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

A G C C G A T G T C G T C G T C T A C C A T G C A T

Polymerase Chain Reaction (PCR)

Part I

Segment of Interest

Page 2: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C C G A T

A T G G T

Part II

Page 3: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C C G A T G T C G T

A G C A G A T G G T

Part III

Page 4: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C C G A T G T C G T

A G C A G A T G G T

Part IV

Page 5: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C C G A T G T C G T

C C G A T G T C G T C G T C T A C C A

G G C T A C A G C A G C A G A T G G T

A G C A G A T G G T

Part IV

Page 6: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C C G A T G T C G T

C C G A T G T C G T C G T C T A C C A

G G C T A C A G C A G C A G A T G G T

A G C A G A T G G T

1 1

1 1

2 2

Part IV

Page 7: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C1

CC1

CC2

CC2

C2

CC1

3’ T C G G C T A C A G C A G C A G A T G G T A C G T A 5’5 ‘ C C G AT G T C G T C G T C T AC C A T G C A T 3’

C C G A T G T C G T C G T C T A C C A T G C A T

C C G A T G T C G T C G T C T A C C A T G C A T

5’ A G C C G A T G T C G T C G T C T A C C A T G C A T 3’T C G G C T A C A G C A G C AG A T G G T

T C G G C T A C A G C A G C AG A T G G T

T C G G C T A C A G C A G C AG A T G G T

Part V

Page 8: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

C1

CC1

CC2

CC2

C2

CC1

3’ T C G G C T A C A G C A G C A G A T G G T A C G T A 5’5 ‘ C C G AT G T C G T C G T C T AC C A T G C A T 3’

C C G A T G T C G T C G T C T A C C A T G C A TG G C T A C A G C A G C A G A T G G T

C C G A T G T C G T C G T C T A C C A T G C A TG G C T A C A G C A G C A G A T G G T

5 ‘ C C G A T G T C G T C G T C T A C C A 3’

G G C T A C A G C A G C A G A T G G T

5’ A G C C G A T G T C G T C G T C T A C C A T G C A T 3’T C G G C T A C A G C A G C AG A T G G T

T C G G C T A C A G C A G C AG A T G G TC C G A T G T C G T C G T C T A C C A

T C G G C T A C A G C A G C AG A T G G TC C G A T G T C G T C G T C T A C C A

Part V

Page 9: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

3’ T C G G C T A C A G C A G C A G A T G G T A C G T A 5’5 ‘ C C G AT G T C G T C G T C T AC C A T G C A T 3’

C C G A T G T C G T C G T C T A C C A T G C A TG G C T A C A G C A G C A G A T G G T

C C G A T G T C G T C G T C T A C C A T G C A TG G C T A C A G C A G C A G A T G G T

5 ‘ C C G A T G T C G T C G T C T A C C A 3’

G G C T A C A G C A G C A G A T G G T

5’ A G C C G A T G T C G T C G T C T A C C A T G C A T 3’T C G G C T A C A G C A G C AG A T G G T

T C G G C T A C A G C A G C AG A T G G TC C G A T G T C G T C G T C T A C C A

T C G G C T A C A G C A G C AG A T G G TC C G A T G T C G T C G T C T A C C A

C1

CC1

CC2

CC2

C2

CC1

Part V

Page 10: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

Part V

16

1 1

3 3

4 4

Page 11: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

32

1 1

4 4

11 11

Part VI

Page 12: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

Part VII

Page 13: A  G  C  C  G A  T  G  T  C  G  T  C  G  T  C  T  A C  C  A  T  G C  A  T

Part VII

2

4

8

16

32

64

2048

2,097,152

1

1

11

11

11

1 1

1

11

1

1

1

0

1

0 0 0

1

1 1

0 0

2 2

3 3

4 4

5 5

10 10

20 20

4 4

11 11

26 26

1013 1013

1,048,555