(980 nm) uc(550 nm) trypan blue 6 h a;49 %) ypo 4sogalabo.jp/ptrcar/h19ptrc_2.pdf · 2010-06-12 ·...
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(980 nm ) U C (550 nm ) Trypan B lue 6 h
100 % 24 h 70 %72 h 3 Y 38 %
45 % ( C ;42 % , G ;44 % , A ;49 % ) Y PO 4
75 %
H 5 Y 2O 3: Er U C6 100
24 70 72 40 50 Y PO 4
75
1) Fang Lim et al, "In V ivo and Scanning Electron M icroscopy Im aging of U pconverting N anophosphors in C aenorhabditis elegans," Nano Letters, 6 [2] (2006)169-174. 2) D avid Schubert et al, "C erium and yttrium oxide nanoparticles are neuroprotective," Biochemical and Biophysical Research Communications, 342 (2006) 86-91.
100
80
60
40
20
0
Sam plesY C G A P
Y 2O 3: Er
Y 2O 3: Er(a) (c) (980 nm ,550 nm)
(FB I)
FB I
FB I
in vivo FB I
nm 500nm
980 nm 1550 nm
Y PO 4: Er in vivo
Y PO 4: Er
m ol 10
K 3PO 4 1100 1
X (FT-IR ) pH 3 H C l 2
pH
5 m ol/l
X
FT-IR (SEM ) 980 nm
Y PO 4: Er O P-50
Y PO 4: Er
U C 980
nm 565 nm Y 2O 3:Er
FT-IR Y PO 4
Y PO 4
K 3Y (PO 4)22 Y PO 4 Y PO 4
K 3Y (PO 4)2 Y PO 4
K 3Y (PO 4)2 K 3(Y PO 4)2Y PO 4: Er FT-IR X
Y PO 4 Y PO 4
Y PO 4 10 Y PO 4
480
Y PO 4 Y PO 4
SEM 500 nm Y PO 4:Er 980 nm
1550 nm 125m W
1s 3(a),(b)
Y 2O 3: Er 900m W 1s 980 nm
U C ( 4(c),(d)
K 3PO 4 500
nm Y PO 4: Er Y PO 4: Er
Y 2O 3: Er
400 500 600 700 800 9000
0.5
1
w avenum ber(cm-1)
Y PO 4Y PO 4
10 20 30 40 50 600
1000
2000
2 (degree)
Y PO 4Y PO 4K 3Y (PO 4)2
3
(a) (b) 4 U C
(a) (b)
T
A ILIM /IC O S T
elongation (1) elongation
T elongation
A ILIM /IC O S T elongation
A ILIM /IC O S T A ILIM /IC O S
elongation C EM T PB T
T elongation
T m R N A
K IF3A K IFC 3 K IF5B K IF9 K LC (2)
dom inant negative A ILIM /IC O S C EM
A ILIM /IC O S K IF3A elongation
K IF3A dom inant negative PB T (Fig.
1)
K IF3A PA R 3 aPK C
(3) G FP-aPK C
C EM elongation
G FP-aPK C T elongation PA R 3 aPK C
dom inant negative PA R 3 aPK C
elongation (Fig. 2)
T elongation K IF3A PA R 3 aPK C
T K IF3A PA R 3 aPK C T
H U V EC
(1) O kam oto N ., N ukada Y., Tezuka K ., et al., Int Immunol 16, 1515-22 (2) R iley L. J, M ao M ., K obayashi S., et al., Proc. Natl Acad. Sci. USA 99, 11790-11795 (3) N ishim ura T., K ato K ., Yam aguchi T., et al., et al, Nat Cell Biol 6, 328-34
Fig.1 K IF3A dom inant negative T elongation
C EM PB T K IF3A w ild type (w t) dom inant negative (D N ) m ock
elo n g atio n
a
b.
c. d.
Fig.2 PA R , aPK C dom inant negative T elongation
c.a. b. d.s.
C EM PB T PA R 3 aPK C w ild type (w t) dom inant negative (D N )
m ock elongation
A B C
K 2
K 2
side population (SP) A B C
H oechst
SP 1 106 K 2 A B C
50 M verapam il 37 30
20 g/m l H oechst 37 60 1 g/m l propidium iodide (PI) 37
10 PI
H oechst SP
K 2 activin A FG F2 H G F
K 2
poly-H em dish 15 FC S-D M EM 2
dish 100 ng/m l activin A FG F2 1 % FC S-D M EM /F12
3 10 ng/m l H G F 1% D M SO 10 %
FC S-D M EM /F12 5 10-7 M
2 K 2 R N A
4K 2 SP K 2
61% SP (Fig.1)
H oechst A B C verapam il SP
0.3% SP
Fig. 1 Existence of SP cells in rat hepatom a K 2 cells.
K 2 K 2 2
activin A FG F2 3
H G F D M SO 5
7 20% , 3%
2 TATsox17 pdx1
1 K 2
K 2
K 2
ribosom e inactivating protein (R IP)
(Aralia elata) aralin
N- A B(1) A ralin R IP
ricin aralin R IP
aralin
aralin
aralin
A ralin C y3 0.1 M N aH C O 3 4 30
G 50 C y3-aralin B SA Lys C y3
C y3-B SA C y3-Lys A ralin
W I-38 VA -13
H eLa H oechst33342 C y3-aralin C y3-B SA 4
15 aralin
H eLa C y3-aralin 10 aralin
aralin Far W estern blot H eLa
SD S-PA G E
aralin aralin H R P
X aralin
aralin H eLa
aralin Far W estern blot 4
A ralin C y3-aralin H eLa VA -13
W I-38 C y3-aralin aralin
(Fig1) C y3-aralin
aralin aralin C y3-aralin
C y3-aralin aralin
H eLa Far W estern blot 100
kD a A ralin
100 kD a aralin
Cy3 Hoechst Phase Merge
Fig1 C y3-aralin
A ralin 100 kD a
A ralin
2. Y 2O 3:Er SEM ((a) PEG ,
(b)PEG (PEG 0.019 chains/nm 2))
3 . PEG Y 2O 3:Er (PEG
0.019 chains/nm 2) U C
(980 nm )
1) 2nd W orkshop on P hotolum inescence in R are E arths: P hotonic M aterials and D evices
(P R E’07) (Tokyo U niv. of Sci.)Yu Saito, K . Shim izu, M . Shim izu, T. K onishi and K . Soga
“A pplication of rare-earth-doped phoshpors for N IR bioim aging” (2007/5/31-6/1, Trento, Italy)
2) M aterials Science and Technology 2007 C onference and E xhibition (M S& T ’07) (Tokyo U niv.
of Sci.) K ohei Soga, Yu Saito, K azuaki Shim izu, Yoshihito O kum ura and Tom oya K onishi,
“A pplication of R are-E arth D oped C eram ic N anophoshpors for B ioim aging under N IR
E xcitation” (2007/9/16-20, D etroit, M ichigan, U SA ).
3) Second International Sym posium on A tom ic Technology (Tokyo U niversity of Science)
K ohei Soga, "D evelopm ent of N IR B ioim aging System s by “Polyscale” T hechnologies Starting
at A tom ic Technologies," (2007/10/1-2, Aw aji, H yogo).
4) Second International Symposium on Atomic Technology (Tokyo Univ. of Sci., Univ. of Tsukuba) N. Venkatachalam1, Yu Saito1, K. Soga1 and Y. Nagasaki2, “Y2O3 Nanophoshpors Prepared by Enzymatic Decomposition for Upconversion Bioimaging" (2007/10/1-2, Awaji, Hyogo).
tional Symposium on Atomic Technology (Tokyo Univ. of Sci., Anan Col. Tech.*) 5) Second Interna
Yoshihito OKUMURA, Yu SAITO, Kazuaki SHIMIZU, Tomoya KONISHI* and Kohei SOGA "Novel
Fabrication Route for Yittrium Oxide Nanoparticles by Using Phosphate Salt" (2007/10/1-2, Awaji,
Hyogo).
6) B ioceram ics 20 (Tokyo. U niv. of Sci.) K . Soga, Y. Saito, K . Shim izu, R . K unibonu and J.
C hiba “R are-E arth-D oped C eram ic N anophosphors for B ioim aging” (2007/10/24-26, N antes,
F rance).
Deregulated expression of a novel component of TFTC/STAGA histone acetyltransferase
complexes, rat SGF29, in hepatocellular carcinoma: possible implication for the oncogenic
potential of c-Myc Nobuya Kurabe, Yuko Komiya, Seiichi Mochizuki, Kumiko Katagiri, Akinori Sugiyama, Yasushi Kawasaki, Fumio
Tashiro.
Department of Biological Science and Technology, Tokyo University of Science
Department of Biological Science and Technology, Tokyo University of Science