37~ PATENT ABSTRACTS

other to form resolved rows on a support sequence homologies to immunoglobulin V and medium, which comprises a process including: C genes. Both the T cell receptor protein and its (I) determining on each of the resolved rows a subunits may be produced from the cDNA scanning line for signal processing; (2) detecting clones. The protein molecules may be further on each of the resolved rows sampling points on used for the production of T-cell clone specific said scanning line; and (3) comparing and iden- antibodies. tifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, 4873191 ademine, thymine and cytosine; said process being applied to digital signals corresponding to G E N E T I C T R A N S F O R M A T I O N O F an autoradiograph having the locational in- Z Y G O T E S formation on the radioactively labeled synthetic products, said digital signals being obtained by Thomas E Wagner, Peter Hoppe assigned to the use of a stimulable phosphor sheet or by the Ohio University use of a radiosensitive material.

Genetic transformation of a zygote and the em- 4873187 bryo and mature organism which result there-

from is obtained by placing or inserting exogenous genetic material into the nucleus of

B I F U N C T I O N A L D N A - P R O T E I N the zygote or into any genetic material which ul- C O N J U G A T I N G A G E N T timately forms at least a part of the nucleus of the

zygote. It is preferred that the exogenous genetic Floy Taub assigned to Digene Diagnostics In- material be added to a pronuclei of the zygote corporated and is particularly preferred that it be added to

the male pronucleus of the zygote. Thereafter, A cationic detergent having a positively charged the zygote is allowed to undergo differentiation group and a hydrophobic group is used to con- and development into the organism. The jugate an enzymatically active enzyme molecule genotype of the zygote and the organism which with a single-stranded nucleic acid molecule. The results therefrom will include the genotype of the conjugate may be used to detect the presence of exogenous genetic material and the exogenous nucleic acid molecules having a nucleotide genetic material will be phenotypically ex- sequence which is complementary to that of the pressed. The invention can be utilized in a variety single-stranded molecule of the conjugate, of ways including, for example, animal and plant

breeding to modify or create new species, it can be used in ¢pigenetics and in the understanding

4873190 and treatment of genetic diseases.

H E T E R O D I M E R I C T L Y M P H O C Y T E R E C E P T O R 4873192

Haruo Saito, David Kranz, Herman N Eisen, P R O C E S S F O R S I T E S P E C I F I C Susumu Tonegawa assigned to Massachusetts M U T A G E N E S I S W I T H O U T Institute of Technology P H E N O T Y P I C S E L E C T I O N

Disclosed is a beterodimeric T lymphocyte Thomas A Kunkelassigned to The United States receptor comprising an alpha and a beta subunit, of America as represented by the Department of Each subunit consists of a signal peptide, Health and Human Services variable, joining, constant, transmembrane, and cytoplasmic regions. The two subunits are con- The present invention discloses several DNA nected by a disulfide bond between cysteine mutagenesis processes using a DNA template residues located between the constant and trans- containing several uracil residues in place of membrane region. The structure, amino acid, thymine, which can be applied without selection and nucleotide sequence of the lymphocyte techniques to produce altered DNA sequences receptor were determined using cDNA clones with approximately 10-fold greater efficiency derived from a functional murine cytotoxic T than current methods of site-specific lymphocyte clone. The genes corresponding to mutagenesis. This template has relatively normal these cDNA are expressed and rearranged coding potential in the in vitro reactions typical specifically in T cells and have significant of standard site-directed mutagenesis protocols