4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · bunyaviridae caliciviridae...

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0 1000 2000 3000 0 50000 100000 150000 200000 250000 genlens$len count Figure S1 Genome length histogram. The genome bin cutoff sizes (red lines) shown on ezVIR plots (data point circumferences) were chosen to separate the three major groups of virus genome lengths. These default values of 4,000 bp 20,000 bp and 40,000 bp can be changed by the user to match other custom databases if needed. 4,000 bp 20,000 bp 40,000 bp Genome length (bp) 0 500 1000 1500 2000 0 5000 10000 15000 20000 genlens$len count 4,000 bp 20,000 bp enlarged region: 0 – 20,000 bp

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Page 1: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

0

1000

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0 50000 100000 150000 200000 250000genlens$len

count

Figure S1 Genome length histogram. The genome bin cutoff sizes (red lines) shown on ezVIR plots (data point circumferences) were chosen to separate the three major groups of virus genome lengths. These default values of 4,000 bp 20,000 bp and 40,000 bp can be changed by the user to match other custom databases if needed.

4,000 bp

20,000 bp

40,000 bp

Genome length (bp)

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count

4,000 bp 20,000 bp

enlarged region: 0 – 20,000 bp

Page 2: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

Herpes-­‐2  

Herpes-­‐1  

Herpes-­‐1  coverage  footprint  

Herpes-­‐2  coverage  footprint  

sliding window of [50 bp]

genome coverage (area under the curve)

genome

Figure S2 Sliding window calculation for maximum depth. The sliding window allows ezVIR to better highlight subtle differences in virus genome coverage (for the Y-axis values on ezVIR plots). Here, while the read coverage (red and blue curves) for two genomes have the same maximum ‘depth’ of coverage, Herpes-1 has a larger footprint and higher read count within the sliding window. The value used for the Y-axis in ezVIR plots is the maximum of the rolling mean in this 50 bp window. In other words, a 50 bp window is rolled along the genome. For each 50 bp window, the average number of reads (coverage) is computed. The maximum average coverage value that is found is used for the Y-axis value for that genome. This measure performs better than ‘average depth’ or ‘overall maximum depth’ calculations, where (for Herpes-1) a similar depth value would have to be divided by a relatively larger denominator.

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maximum depth of coverage

Page 3: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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< 4,000 > 4,000

> 20,000

> 40,000

AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Herpes_simplex_2

Herpes_simplex_1

Cytomegalovirus

JC_polyomavirus

specimen−01PHASE−1 | viruses with > 0 % genome coverage

specimen−01_cut−0_p1−plot.pdf | 2014−06−25 14:53

% genome coverage

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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 4: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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< 4,000 > 4,000

> 20,000

> 40,000

AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

BK_polyomavirus

Herpes_simplex_1Cytomegalovirus

JC_polyomavirus

TT_virus

specimen−02PHASE−1 | viruses with > 0 % genome coverage

specimen−02_cut−0_p1−plot.pdf | 2014−06−25 14:56

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 5: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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< 4,000 > 4,000

> 20,000

> 40,000

AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_1Herpesvirus_3Cytomegalovirus

Herpesvirus_6AHerpesvirus_6B

Herpesvirus_7

specimen−03PHASE−1 | viruses with > 0 % genome coverage

specimen−03_cut−0_p1−plot.pdf | 2014−06−25 14:57

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 6: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_1Cytomegalovirus

Herpesvirus_6A

Herpesvirus_6BHerpesvirus_7

Papillomavirus

TT_virus

specimen−04PHASE−1 | viruses with > 0 % genome coverage

specimen−04_cut−0_p1−plot.pdf | 2014−06−25 14:58

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 7: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

BK_polyomavirus

Herpes_simplex_1Cytomegalovirus

Herpesvirus_6A

Herpesvirus_6BHerpesvirus_7

Micro_TT_virus

TT_virus

specimen−05PHASE−1 | viruses with > 0 % genome coverage

specimen−05_cut−0_p1−plot.pdf | 2014−06−25 14:59

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 8: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Epstein−Barr_virusHerpesvirus_6AHerpesvirus_6B

Micro_TT_virus

TT_virus

specimen−06PHASE−1 | viruses with > 0 % genome coverage

specimen−06_cut−0_p1−plot.pdf | 2014−06−25 15:00

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 9: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

CytomegalovirusHerpes_simplex_1

Herpesvirus_6AHerpesvirus_6B

Herpesvirus_7

Micro_TT_virus

TT_virus

specimen−07PHASE−1 | viruses with > 0 % genome coverage

specimen−07_cut−0_p1−plot.pdf | 2014−06−25 15:01

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 10: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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> 20,000

> 40,000

AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_2

Herpes_simplex_1

Cytomegalovirus

specimen−08PHASE−1 | viruses with > 0 % genome coverage

specimen−08_cut−0_p1−plot.pdf | 2014−06−25 15:20

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 11: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Parvovirus_B19

Herpes_simplex_1Cytomegalovirus

Herpesvirus_6AHerpesvirus_6B

Herpesvirus_7

specimen−09PHASE−1 | viruses with > 0 % genome coverage

specimen−09_cut−0_p1−plot.pdf | 2014−06−25 15:20

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 12: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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Total covered length (bp):

< 4,000 > 4,000

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Coxsackievirus

EchovirusEnterovirus

Herpes_simplex_1

RhinovirusMerkel_cell_polyomavirus

specimen−10PHASE−1 | viruses with > 0 % genome coverage

specimen−10_cut−0_p1−plot.pdf | 2014−06−25 15:18

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 13: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Rhinovirus

specimen−11PHASE−1 | viruses with > 0 % genome coverage

specimen−11_cut−0_p1−plot.pdf | 2014−06−25 15:19

% genome coverage

Tom
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Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 14: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Coxsackievirus−B

Echovirus

Enterovirus

Epstein−Barr_virus

Coxsackievirus

Herpes_simplex_1

CytomegalovirusMeasles

specimen−12PHASE−1 | viruses with > 0 % genome coverage

specimen−12_cut−0_p1−plot.pdf | 2014−06−25 15:02

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 15: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_1Cytomegalovirus

Rhinovirus

Measles

specimen−13PHASE−1 | viruses with > 0 % genome coverage

specimen−13_cut−0_p1−plot.pdf | 2014−06−25 15:03

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 16: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_1Cytomegalovirus

Metapneumovirus

specimen−14PHASE−1 | viruses with > 0 % genome coverage

specimen−14_cut−0_p1−plot.pdf | 2014−06−25 15:04

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 17: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Herpes_simplex_1Metapneumovirus

Parainfluenza_3

Merkel_cell_polyomavirus

specimen−15PHASE−1 | viruses with > 0 % genome coverage

specimen−15_cut−0_p1−plot.pdf | 2014−06−25 15:05

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 18: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Herpes_simplex_1Cytomegalovirus

Rhinovirus

specimen−16PHASE−1 | viruses with > 0 % genome coverage

specimen−16_cut−0_p1−plot.pdf | 2014−06−25 15:07

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 19: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Epstein−Barr_virus

Hepatitis_G

Herpes_simplex_1Cytomegalovirus

Rhinovirus

Influenza_A

specimen−17PHASE−1 | viruses with > 0 % genome coverage

specimen−17_cut−0_p1−plot.pdf | 2014−06−25 15:10

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 20: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

0 25 50 75 100

Epstein−Barr_virus

HIV−1

Herpes_simplex_1Cytomegalovirus

Lymphadenopathy_virus

T_Cell_Lymphotropic_Virus

specimen−18PHASE−1 | viruses with > 0 % genome coverage

specimen−18_cut−0_p1−plot.pdf | 2014−06−25 15:15

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 21: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Epstein−Barr_virus

Hepatitis_C

specimen−19PHASE−1 | viruses with > 0 % genome coverage

specimen−19_cut−0_p1−plot.pdf | 2014−06−25 15:16

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 22: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Parainfluenza_1

Parainfluenza_3Parainfluenza_4

Parechovirus

Rhinovirus

Measles

specimen−20PHASE−1 | viruses with > 0 % genome coverage

specimen−20_cut−0_p1−plot.pdf | 2014−06−25 15:21

% genome coverage

Tom
Typewritten Text
Figure S3. ezVIR phase-1 reports. Note that this is the default view where all detected viruses (> 0% genome coverage) are displayed. Users have the option to define a minimum % genome coverage that can help remove background signals.
Page 23: 4,000 bp 4,000 bp 20,000 bp 20,000 bp 40,000 bp · 2014-08-15 · Bunyaviridae Caliciviridae Coronaviridae Flaviviridae Hepadnaviridae Hepeviridae Herpesviridae Orthomyxoviridae Papillomaviridae

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Epstein−Barr_virus

Hepatitis_G

Herpes_simplex_1Cytomegalovirus

Rhinovirus

Influenza_A

specimen−17PHASE−1 | viruses with > 0 % genome coverage

specimen−17_cut−0_p1−plot.pdf | 2014−03−04 11:54

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Epstein−Barr_virus

Hepatitis_G

Herpes_simplex_1Cytomegalovirus

Rhinovirus

Influenza_A

specimen−17PHASE−1 | viruses with > 0 % genome coverage

specimen−17_cut−0_p1−plot.pdf | 2014−06−25 15:10

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Coxsackievirus−B

Echovirus

Enterovirus

Epstein−Barr_virus

CoxsackievirusHerpes_simplex_1

Cytomegalovirus Measles

specimen−12PHASE−1 | viruses with > 0 % genome coverage

specimen−12_cut−0_p1−plot.pdf | 2014−03−04 11:47

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Coxsackievirus−B

Echovirus

Enterovirus

Epstein−Barr_virus

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Herpes_simplex_1

CytomegalovirusMeasles

specimen−12PHASE−1 | viruses with > 0 % genome coverage

specimen−12_cut−0_p1−plot.pdf | 2014−06−25 15:02

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Coxsackievirus

EchovirusEnterovirus

Herpes_simplex_1

RhinovirusMerkel_cell_polyomavirus

specimen−10PHASE−1 | viruses with > 0 % genome coverage

specimen−10_cut−0_p1−plot.pdf | 2014−03−04 12:02

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Coxsackievirus

EchovirusEnterovirus

Herpes_simplex_1

RhinovirusMerkel_cell_polyomavirus

specimen−10PHASE−1 | viruses with > 0 % genome coverage

specimen−10_cut−0_p1−plot.pdf | 2014−06−25 15:18

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6

197166

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mappping to: Human rhinovirus 66 strain ATCC VR−1176− complete genomegenome ID: gi|217316426|gb|FJ445148.1| | May 02, 2014 at 02:12 PM

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AdenoviridaeArenaviridaeAstroviridaeBunyaviridaeCaliciviridaeCoronaviridaeFlaviviridaeHepadnaviridaeHepeviridaeHerpesviridaeOrthomyxoviridaePapillomaviridaeParamyxoviridaeParvoviridaePicornaviridaePolyomaviridaeReoviridaeRetroviridaeRhabdoviridaeTogaviridaeOtherpadding

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Herpes_simplex_2

Herpes_simplex_1

Cytomegalovirus

JC_polyomavirus

specimen−01PHASE−1 | viruses with > 0 % genome coverage

specimen−01_cut−0_p1−plot.pdf | 2014−03−04 11:37

% genome coverage

Measles in specimen-12 C

JC polyomavirus in specimen-01 A

HRV in specimen-10 B

HRV in specimen-17 D

specimen shown positive for HRV *

*

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01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20specimen ID

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mappping to: JC polyomavirus strain JCVCPN1− complete genomegenome ID: gi|383932066|gb|JQ823124.1| | May 02, 2014 at 02:58 PM

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317

100

100.5

101

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01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20specimen ID

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mappping to: Human rhinovirus 73− complete genomegenome ID: gi|95102499|gb|DQ473492.1| | May 02, 2014 at 03:03 PM

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01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20specimen ID

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mappping to: Measles virus strain MVi−New Jersey.USA−45.05− complete genomegenome ID: gi|347990581|gb|JN635408.1| | May 02, 2014 at 03:02 PM

specimen shown positive for Measles *

*

specimen shown positive for HRV *

*

Tom
Text Box
Figure S4. Cross contamination analysis (CCA) for four specimens. In all cases, the contaminating strain originates from the neighboring specimen preparation. Reference specimen is indicated by a red arrow. Note Y-axis is log scale.