Better than 2D,

In vivo, cell behavior results from the integration of different biochemical signals and mediators and from mechano-structural guidance, a relationship that has been largely ignored in 2D culture systems. «Classical» in vitro 2D cellular assays are performed on uniform adhesive substrates that can introduce considerable but unnoticed variability in cell function. With 2D+ adhesive micropatterns, the topology of cell adhesion can be finely controlled, as well as the spreading and 3D shape of cultured cells in single- or multi-cellular configurations.

a new dimension in cell culture

assay InnovationStructural guidance brought by our adhesive micropatterns results in predictive cell organization and behavior. thus, small perturbations induced by drugs or any other external stimuli can be immediately detected and quantified, instead of being lost in the variability of a classical 2D cell culture.

assay Optimizationnormalization of cell morphology reduces readout variability, increasing sensitivity and robustness. normalized cell architecture also means that an average cell can be calculated and be used as a stable reference Cell* between experiments and platforms.

assay resuscitationthe exquisite control of each cell’s microenvironment opens new possibilities for working with difficult and complex assays to redesign them in a standardized, quantitative and simplified manner.

Cell functions can be restored, stem cell differentiation and fate can be controlled, polarized 3D structures can be built..

• Provides structural guidance • Increases relevance• normalizes single and multi-cellular structures

* www.CYTOO.com/referenceCell

Contact us to discuss your application: www.CYTOO.com/contact

CYTOO Cell architects7 parvis Louis néel, Bht, BP 50 - 38040 Grenoble FranCe, +33 (0)4 38 88 47 05

CYTOO Inc harvard Square, One Mifflin Place, Suite 400Cambridge, Ma 02138 , USa +1 617 674 7711 vi

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exploratory Solutions Bringing together our ecosystems with the customer’s cellsWe offer exploratory 2D+ Solutions to guide our customers through the initial exploration of our technology.Our Jungle plates are designed to offer a range of ecosystems for each cell type, to observe their behavior and function in a wide variety of microenvironment conditions and applications. typical exploratory projects can be fulfilled in less than a month, with experimental work spanning a few days either at CYtOO or at the customer’s site.

assay development A Darwinian approach to select the best ecosystemCYtOO also offers full assay development services, integrating the choice of cell line and biomarker, along with the best microenvironment geometry and adhesion protein. the latter is an iterative process which involves custom pattern designs and layout to best elicit the targeted function or phenotype, and fine tuned to offer the widest dynamic range in terms of readout with appropriate positive and negative controls.In the final iteration, the customized CYtOOplates can be manufactured in large volumes to streamline into screening.

Screening Seamless ramp-up into larger campaignsCYtOO is equipped with state-of-the-art high Content analysis instruments to run small and medium screens in house. We also develop innovative algorithms to extract maximal performance from our 2D+ technology. We are also equipped with clean rooms, large scale manufacturing, and stringent quality control standards, to turn out high volumes of CYtOOplates for any sized screen.

CYtOO’s Custom Solutions brings expertise, resources and technology to solve the issues of current bottlenecks in cell based assay development and screening. Our Solutions span initial exploration, feasibility, assay development and screening, both at CYtOO or at our customer’s facilities.Custom Solutions begin with an in-depth analysis of the customer’s assay, with experienced PhD-level experts of our technology. We work together on a project and workflow description to define objectives, timeline and budget.Our Custom Solutions fall into 3 main categories, but each Solution is unique, confidential and tailored to the needs of our customer.

Solutions