HPR-40 DSA Membrane Inlet Mass Spectrometers for Dissolved Species Analysis

HPR-40 DSA Membrane Inlet Mass Spectrometers for Dissolved Species Analysis

Membrane Inlet Mass Spectrometry (MIMS) enables analyses of dissolved gases and volatile compounds from bulk samples.

fermentation process analysisenvironmental monitoringwater analysis

Membrane Inlet Mass Spectrometry (MIMS)

Applications:XX Water analysis in Estuary, River or Reservoir (Denitrification)

XX Soil core analysis

XX Fermentation process analysis

XX Groundwater contamination studies

XX Methane production control

XX Microbiological/Enzyme activity studies

XX Environmental monitoring

XX Electrochemical Analysis (DEMS)

Membrane Inlet MS - Applications

Pervaporation

Vacuum (Vapour phase) Liquid phase

PolydimethylsiloxaneMembrane

(gas permeable)

To QuadrupoleMass Spectrometer

Gas/Vapour Molecule

Desorption

Diffusion

Partitioning

Example Data

Denitrifi cation StudiesThe HPR-40 DSA was used to investigate denitrification by Pseudomonas stutzeri in a static lakewater column. Continuous real-time measurement of gases enabled the dynamics of the process to be investigated. Concentrations of 17 mmol/L nitrate and 10 mmol/L nitrite were identified as optimal for denitrification under nutrient-limited conditions (i.e., produced the highest concentrations of N2). Available carbon was the major rate-limiting factor in lakewater when nitrate or nitrite was present. No stratification of the process with depth was observed, and aerobic denitrification was apparent under all the conditions employed.

PPT Level Detection of Volatiles in Water The HPR-40 DSA was used to detect trace amounts of CH3SCH3 (Dimethylsulfide DMS) in ocean water. DMS is a naturally occurringsubstance produced from micro-organisms and related to globalclimate change and regulation.

The amounts which were detected in this application go down to 60 ppt(parts per trillion), which corresponds to dilute concentrations of DMSof less than 1 nML-1.

The diagrams show the calibration curve and raw data obtained fromDMS solutions in different concentrations.

Denitrifi cation by Pseudomonas stutzeri in a sterilelake water microcosm supplemented with succinateand nitrate.

JR Firth and C Edwards, Journal of Applied Microbiology, 88, 853-859.

Data obtained with assistance from P.D.Tortell, Department of Botany, University of British Columbia.

24

750

Tim 108 cells ml–1e (h)

[N2]

(μm

ol l–1

)

00

2016128

(a)

300

150

450

4

600

2·75x108

2·06x1081·50x108

1·88x108

1·04x108

6·90x107

[N2]

(μm

ol l–1

)Pe

ak

(b)

0 0.5 1.0 1.5 2.0 2.5 3.0

800

700

600

500

400

300

200

100

024

750

Tim 108 cells ml–1e (h)

[N2]

(μm

ol l–1

)

00

2016128

(a)

300

150

450

4

600

2·75x108

2·06x1081·50x108

1·88x108

1·04x108

6·90x107

[N2]

(μm

ol l–1

)Pe

ak

(b)

0 0.5 1.0 1.5 2.0 2.5 3.0

800

700

600

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0

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5000DM

S si

gnal

inte

nsity

cou

nts/

sec

(c/s

)

00:00:00 00:07:12 00:14:24 00:21:360

(HAL 101/3F, PIC mode) 40nML-1

1nML-1

4nML-1

------ Dilute DMS peak at 62 amu

40

35

30

25

20

15

10

5

Sign

al in

tens

ity (c

/s)x

103

0 250

1 4 10 40

500 750 1000 1250 1500 1750 2000 2250 2500 27500

Signal intensity fromHAL 101/3F in PIC mode

DMS signal at 62 amu

DMS dilute concentration level parts per trillion (ppt)

nML-1

1 ppb

40000

35000

30000

25000

20000

15000

10000

5000DM

S si

gnal

inte

nsity

cou

nts/

sec

(c/s

)

00:00:00 00:07:12 00:14:24 00:21:360

(HAL 101/3F, PIC mode) 40nML-1

1nML-1

4nML-1

------ Dilute DMS peak at 62 amu

40

35

30

25

20

15

10

5

Sign

al in

tens

ity (c

/s)x

103

0 250

1 4 10 40

500 750 1000 1250 1500 1750 2000 2250 2500 27500

Signal intensity fromHAL 101/3F in PIC mode

DMS signal at 62 amu

DMS dilute concentration level parts per trillion (ppt)

nML-1

1 ppb

Technical data

Mass ranges, amu: 1-200 / 1-300 amu

Sensitivity: 100% to 60 ppt (species dependent) subject to spectral interference

Speed: Up to 650 measurements/second

Software: MASsoft Professional

Windows 7/8/10 compatible

Interface: Ethernet/USB/Serial (RS-232) connections

Detector: Dual Faraday/Channeltron Electron Multiplier

Analogue input: 8x (optional)/16 bit

Analogue output: 8x (optional)/14 bit

Digital input: 8x

Digital output: 8x, 24 V

Further system options: Application specific inlets

Multi-stream sampling

Dimensions (L x W x H), mm: 495 x 539 x 393 mm

Weight, kg: Typically 33 kg and external membrane backing pump 7 kg

Power requirement: 110/220/240 V AC, 50/60 Hz, 1.2 kVA

HPR-40 DSA with probe inlet (external membrane backing pump not shown) Dimensions in mm

Inlet options

FLOW-THROUGH PROBE

The Flow-through Probe is directly mounted on the MS with two ports to allow flow from an external reservoir.It is used for a wide range of dynamic applications.

CUVETTE CELL

The Cuvette Cell is designed to allow temperature control and illumination of a liquid sample – e.g. for algae studies.

ENZYME KINETICS PROBE

The Enzyme Kinetics Probe was specifically developed to be submerged in enzyme containing samples. All wetted parts are made of glass to avoid sample contamination and side reactions.

TYPE A DEMS CELL*

The Type A DEMS Cell supplies a membrane inlet in close vicinity toan electrode for flexible analyses of electro-chemical reactions or catalysts.

TYPE B DEMS CELL*

The Type B DEMS Cell is designed for optimum performance in analysis of electro-chemical reaction products.

PROBE INLET

The Probe Inlet is a membrane inlet that can be submerged in any liquid or slurry sample. It is used for a wide range of applications.

*For more info on DEMS please see DEMS brochure.

Hiden’s quadrupole mass spectrometer systems address a broad application range in:

Sales Offi ces:We have sales offices situated around the globe. Visit our website for further information.

Hiden Analytical Ltd.420 Europa BoulevardWarrington WA5 7UN England

T +44 [0] 1925 445 225F +44 [0] 1925 416 518E info@hiden.co.ukW www.HidenAnalytical.com

GAS ANALYSIS

dynamic measurement of reaction gas streamscatalysis and thermal analysismolecular beam studiesdissolved species probesfermentation, environmental and ecological studies

SURFACE ANALYSIS

UHV TPDSIMSend point detection in ion beam etchelemental imaging – 3D mapping

PLASMA DIAGNOSTICS

plasma source characterisationetch and deposition process reaction kinetic studiesanalysis of neutral and radical species

VACUUM ANALYSIS

partial pressure measurement and control of process gasesreactive sputter process controlvacuum diagnosticsvacuum coating process monitoring

etch and deposition process reaction kinetic studiesanalysis of neutral and radical species

VACUUM ANALYSIS

TDS 171/3

a broad application range in:

dynamic measurement of reaction gas streams

fermentation, environmental and ecological studies

SURFACE ANALYSIS