204 Impact of maternal diet on peripheral vs hepaticinsulin resistance in young female offspringHye Heo1, Howard Slomko2, Scarlett Karakash1,Fabien Delahaye1, Yongmei Zhao1, DerekHuffman3, Nir Barzilai3, Francine Einstein4

1Albert Einstein College of Medicine/Montefiore Medical Center,Obstetrics & Gynecology and Women’s Health, Bronx, NY, 2AlbertEinstein College of Medicine/Montefiore Medical Center, Pediatrics,Bronx, NY, 3Albert Einstein College of Medicine, Medicine, Bronx, NY,4Albert Einstein College of Medicine/Montefiore Medical Center,Obstetrics & Gynecology and Women’s Health, Bronx, NYOBJECTIVE: To characterize body composition and metabolic pheno-type of young female offspring exposed to maternal western diet andcalorie restriction (CR).STUDY DESIGN: SD female rats were fed 1 of 3 diets: 1) standard chow(Con-M), 2) western diet from 3 wks through gestation/lactation(WD-M) or 3) standard chow from 3 wks until Day 11 of gestation,then calorie restricted (pair-fed 50% kcal/d of Con-M) through lac-tation (CR-M). Litters were culled to 8pups. Female offspring (n�9/grp, Con-F, WD-F and CR-F) were given standard chow after wean-ing. Body composition (microCT) was assessed at 4 and 6 weeks of life.At 9wks, insulin sensitivity was measured by hyperinsulinemic (3mU/kg/min) clamp technique, using somatostatin (1.5ug/kg/min) and3H3-glucose (to follow glucose flux).RESULTS: Maternal calorie intake (kcal/d/kg) and weight were similarin Con-M and WD-M. Litter size, pup and placental weight weresimilar in all groups. At weaning, WD-F weighed more and hadgreater SC/VF ratio compared to Con-F, but no differences in total fat.CR-F weighed less and had less total fat at weaning and did not catchup in growth. By 6wks, weight and fat distribution normalized inWD-F compared to Con-F. Both WD-F and CR-F consumed morecalories for body weight compared to Con-F during the study, butnormalized at 9 wks (Fig 1). Clamp results shown in Table 1. Glucoseinfusion rate was lower in both WD-F and CR-F. While CR-F hadgreater hepatic insulin resistance (less suppression of hepatic glucoseproduction (HGP)), HGP was similar in WD-F compared to Con-F.Peripheral insulin sensitivity (Rd) was lower in WD-F, but this did notreach statistical significance.CONCLUSION: Western diet alone (i.e. without changes in maternalbody weight) leads to insulin resistance in the young female rat off-spring similar to the degree seen with severe maternal CR. Unlikeoffspring of WD dams, offspring of CR dams have greater degree ofhepatic insulin resistance. The mechanism for differences in hepatic vsperipheral insulin resistance requires further study. *p�0.05.

205 Sedentary behavior during pregnancy does notalter maternal fat distribution or insulin actionHye Heo1, Scarlett Karakash1, Derek Huffman2, FabienDelahaye1, Yongmei Zhao1, Nir Barzilai2, Francine Einstein3

1Albert Einstein College of Medicine/Montefiore Medical Center,Obstetrics & Gynecology and Women’s Health, Bronx, NY,2Albert Einstein College of Medicine, Medicine, Bronx, NY,3Albert Einstein College of Medicine/Montefiore Medical Center,Obstetrics & Gynecology and Women’s Health, Bronx, NYOBJECTIVE: To determine the effects of voluntary exercise on insulinaction and body composition in young pregnant and non-pregnantfemale rodents.STUDY DESIGN: Four groups of lean, age-matched SD rats were studied(n�5/group): non-pregnant controls (NPC), non-pregnant seden-tary (NPS), pregnant controls (PC), and pregnant sedentary (PS). PChad continuous voluntary access to running wheels starting Day 1 of a22 day gestation. PS were housed in similar cages without access to therunning apparatus. At day 19, insulin sensitivity was assessed by hy-perinsulinemic-euglycemic (3mU/kg/min) clamp technique, usingsomatostatin (1.5ug/kg/min) and 3H3-glucose (to follow glucoseflux). A bolus of 2-[U-14C]deoxyglucose (20 Ci) was administered 30min before the end of the clamp to determine tissue glucose uptake.To study chronic effects of exercise (as opposed to acute effects), wheelbreaks were placed 18 hrs prior to insulin clamps. Animals were sac-rificed at end of the clamp and tissues were collected and weighed.RESULTS: NPS had similar food intake, but greater visceral fat (3.0�.3v 1.7�.4g; p�0.05) compared to NPC. PS and PC also had similarfood intake, but no significant differences in visceral fat. PS and PChad no differences in pup weight, litter size, or glucose uptake inplacenta, pups or maternal muscle. PC ran approximately 1/4 of thedistance that NPC ran (9,434�1,593 v 36,235�11,517m; p�0.059).During insulin clamps (Table 1), overall insulin sensitivity, measuredby glucose infusion rate (GIR) was not statistically different in NPSand NPC. In NPS, peripheral insulin sensitivity (Rd) was similar, buthepatic glucose production (HGP) was greater compared to NPC.GIR, Rd and HGP were similar in PS and PC.CONCLUSION: Sedentary behavior in non-pregnant, young female ro-dents is associated with increased visceral adiposity and hepatic insu-lin resistance. This effect is not seen in sedentary pregnant animalsmost likely because voluntary activity is decreased in pregnancy.*p�0.05.

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Supplement to JANUARY 2012 American Journal of Obstetrics & Gynecology S103