199: Maternal obesity suppresses placental fatty acid uptake in male offspring

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<ul><li><p>CONCLUSION: Black women had lower levels of triglycerides and fattyacids in the 1st and 2nd trimesters compared to white women, andblack women had higher levels of omega-6 fatty acids at 24-28 weeks.Triglycerides and fatty acids are essential for fetal growth and devel-opment, and higher levels of omega-6 fatty acids have been linked topreterm birth. Further investigation is necessary to determinewhether these lipid differences contribute to race disparities in preg-nancy outcomes.</p><p>198 Prolactin derived vasoinhibins are associated withinflammation in mid-gestationChristy Pearce1, Linah Al-Alem2, John OBrien2, Kristine Lain3,Wendy Hansen2, Thomas Curry21Vanderbilt University, OB/GYN, Nashville, TN, 2University of Kentucky,OB/GYN, Lexington, KY, 3NortonHealthcare, Maternal Fetal Medicine,Louisville, KYOBJECTIVE: Prolactin derived vasoinhibins have been implicated inthe pathophysiology of preeclampsia and peripartum cardiomyopa-thy via a pro-inflammatory mechanism. We sought to determine thepresence and examine the relationship of these prolactin derived va-soinhibins and inflammation in mid-gestation.STUDY DESIGN: Women with a singleton gestation between 16-23weeks without exclusion for preexisting medical conditions were re-cruited as the study cohort.Womenwith preeclampsia at 24-42weekswere recruited as physiologic standards. Serumwas tested for C-reac-tive protein with a turbidimetric assay, prolactin with an immunoas-say, and prolactin derived vasoinhibins via immunoprecipitation andWestern blot. A sample bywhich to normalize vasoinhibin expressionacross different Western blots was selected from one of the recruitedpreeclamptic standards. Statistical analysis included t-test and corre-lation coefficients.RESULTS: Sixty subjects were recruited at an average of 19.9 1.5weeks gestation for the study cohort. The mean age was 22.4 3.6years and mean body mass index (BMI) was 27.9 7.1 kg/m2. Mostwomen were primigravidas (n47, 78.3%) and Caucasian (n43,71.7%). Forty-nine (81.7%) of samples expressed a significant mea-surable optical density of PRL fragment on Western blot. Increasingprolactin derived vasoinhibins and the ratio of vasoinhibins to pro-lactin was associated with increasing C-reactive protein (rho0.3,p0.04 and rho0.3, p0.04). See figure 1. Significant differences inmean C-reactive protein were noted between samples above and be-low the median prolactin derived vasoinhibin optical density(9.78.1 vs. 6.36.1, p0.03) and the median ratio of vasoinhibinsto prolactin (9.98.1 vs. 6.26.1, p0.02).CONCLUSION: Prolactin derived vasoinhibins are associated with in-flammation in mid-gestation. Further examination of these prolactinfragments in larger, high risk cohorts may be instructive in the patho-physiology of obstetrical conditions.</p><p>199 Maternal obesity suppresses placentalfatty acid uptake in male offspringElizabeth Brass1, Kent Thornburg2, Perrie OTierney21OregonHealth &amp; Science University, Obstetrics &amp;Gynecology, Portland,OR, 2OregonHealth &amp; Science University, Heart Research Center, DeptCardiovascularMedicine, Portland, OROBJECTIVE: The fetus is dependent on the placenta for its supply oflong chain polyunsaturated fatty acids (LCPUFA), which are essentialin fetal growth and development. Previous work suggests that mater-nal bodymass index is associatedwith fetal LCPUFAdelivery and thatmales have greater fatty acid requirements than females during devel-opment.We hypothesized thatmale placental fatty acid uptake wouldbe more sensitive to maternal BMI compared to females.STUDY DESIGN: Women were recruited upon admission to Labor &amp;Delivery for cesarean section (n25). At delivery, placental sampleswere collected for fatty acid uptake studies using 14C-labeled oleicacid (OA), arachidonic acid, (AA) and docosahexanoic acid (DHA) inplacental explants. Uptake was calculated as nmol fatty acid/mg pro-tein at 15 minutes. Results were stratified by fetal sex and maternalfirst trimester BMI (normal BMI25, obese BMI26). Women withsignificant co-morbidities were excluded. Dichotomous outcomeswere analyzed using 1 way ANOVA followed by Tukey post-hoc test-ing; p0.05 was used to indicate statistical significance.RESULTS: Placental fatty acid uptake of OA and AA in males of obesewomenwas decreased 62%and 60% respectively compared to normalBMI women (p0.001). In females, placental fatty acid uptake wasnot suppressed in the setting of obesity; OA and AA uptake in normalBMI women was reduced 49% and 48% respectively compared to itsmale cohort (p0.01). There was no difference in DHA uptake be-tween sex or BMI groups. Fatty acid transporter CD36 and bindingprotein FABP5 gene expression levels in males mirrored the fatty aciduptake suppression seen in obesity.CONCLUSION: Placentas from males with obese mothers had sup-pressed uptake of unsaturated fatty acids and expression of theirtransporters, while uptake in females was unaffected by maternalBMI. This data suggest thatmales born to highBMImothersmayhaveinadequate LCPUFA acquisition. Males may pay a higher develop-mental price in the setting of maternal obesity.</p><p>200 Fetoplacental endothelium demonstrate uniqueresponses to physiologic hypoxemiaEmily Su1, Hong Xin1, Chunfa Jie2, Nadareh Jafari3,Matthew Dyson1, Serdar Bulun11Northwestern University Feinberg School ofMedicine, Obstetrics andGynecology, Chicago, IL, 2Northwestern University Feinberg School ofMedicine, Surgery/Organ Transplantation, Chicago, IL, 3NorthwesternUniversity Feinberg School ofMedicine, Center for GeneticMedicine,Chicago, ILOBJECTIVE: Although physiologic, fetoplacental pO2 is relatively hy-poxemic in comparison to postnatal life, ranging from 12-50 mmHg(1.5 - 8%O2). In adults, hypoxic conditions have profound effects onendothelial cell function that are often deleterious. We sought to de-</p><p>Correlation of CRP with prolactin derivedvasoinhibin and ratio of prolactin derivedvasoinhibin to prolactin</p><p>Poster Session I Clinical Obstetrics, Epidemiology, Fetus, Medical-Surgical Complications, Neonatology, Physiology/Endocrinology, Prematurity www.AJOG.org</p><p>S94 American Journal of Obstetrics&amp; Gynecology Supplement to JANUARY 2013</p></li></ul>


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