16th congress in plant pathology

7/22/2019 16th Congress in Plant Pathology

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.

52 16o


53/192

[`p`isfphrk{q, rtvl{sfs` i`h dm\ojxrf `lgdishi}l rs` ktifsnis{l``onknl}rdxl snt B6;9@;: +9)rd i`jjh]pbdhdq k`pntjhn|

U. _fyklilqutzptw ofn ._. Yfuubw

, & ,384 46 . ,

, -

(MIC)

447 B. cinerea.

[QoIHRBosHRHydHRAniHRPhenMRDicMRBenHR, QoIHRBosHRAniHRDicMRBenHR, QoIHR-

BosHRDicMRBenHR] (QoI), (Bos), -

(Hyd), (Ani), (Phen),

(Dic) (en),

7%, 12% 6%, . -

. -

(BenHR) 11%

. (


54/192

Qnphì{q v`pìsfphrk{q, kdgnnjnb_` `l_vldtrfq kds`jj`b}l sfq:,0Bi`h rv]rdhq h`rs`tpxs^q lgdishi{sfs`q rd rsdj]vf snt k|ifs`B6;9@;: +9)`lgdishi}l rsntq o`pdkonhrs]q sfq ùtpnbnl\rfq

snt fjdisphin| nm]nqQ. jptofw1, M. L;2, M. H+2 ofn .[. Sfvfthpfreilw1

1. , , ,

54124,

2. Department of Biology, University of Kaiserslautern, Germany

- (SDHIs) . , -

. - : )

-

(sdh) 25 B. cinerea,

boscalid, )

) SDHIs.

3 (B,C D) sdh

5

(P225F, N230I H272L/R/Y). -

, PIRA-PCR (primer in-

troduced restriction analysis-PCR).

.

SDHIs 30 (5

) 8 .

.

P225F -

. , H272L

boscalid -

. N230I boscalid,fluopyram fluxapyroxad isopyrazam,

bixafen, fenfuram, benodanil carboxin. H272R -

boscalid, isopy-

razam, fenfuram carboxin, fluopyram, bixafen,

fluxapyroxad benodanil. , H272Y

boscalid, isopyrazam,

bixafen, fenfuram carboxin,

fluopyram benodanil ( ).

-

.

54 16o


55/192

lgdishi{sfs` ojfgtrk}l snt k|ifs` B6;9@;: +9)`o{ i`jjh]pbdhdqup\ntj`q i`h gdpknifoh`i^q snk\s`q rd anspthnis{l`

Y. Sfpthjvtutptz, Q. jptofw ofn .[. Sfvfthpfreilw

, , ,54124,

Botrytis cinerea -

. -

( )

( , ). 1.160

cypro-

dinil (), fenhexamid (), fludioxonil (-

), carbendazim (), pyraclostrobin (QoIs) boscalid (SDHIs).

. -

. -

(48.9 % ), - (57.4%

),

(52.8 % ). -

-

, , Qol SDHI -

57% .

.

boscalid

(46.7% 76.8% -

, ). , -

boscalid

0.8, 9.8 13.2% , , -

. boscalid -

PIRA-PCR. sdhB

H272R.

55


56/192

Upnr`pknrshi{sfs` i`h ls`bxlhrshi^ hi`l{sfs` rsdjdv}l snt k|ifs`

B6;9@;: +9)kd kds`jj`b]q rsn bnl_hn sfq `utpnbnl\rfq snt

fjdisphin| nm]nq

Q. jptofw1, Y. Sfpthjvtutptz1, .. Ufvohptz2 ofn . Sfvfthpfreilw1

1. , , ,

. 269, 54124,

2. , , 75, -

118 55

(SDHIs) - . , , - .

B

(sdhB).

H272Y/R/L, N230I P225F ,

Botrytis

cinerea. : )

in vitro, )

in vivo ) in vitro

. ,

fluopyram boscalid. -

230 -

272 .

H272R, H272L

P225F . -

-

. H272R . H272Y/L,

N230I P225F

.

4

-

. -

boscalid

H272L P225F . fluopyram 1o

56 16o


57/192

P225F . -

272R -

,

. -

- SDHIs

sdhB .

57


58/192

Ztsno`gnjnbhi{q i`h knph`i{q v`p`isfphrk{q rsdjdv}l snt k|ifs`

+33


59/192

Sdh , -

boscalid (RF: 300) -

fluopyram (RF: 10).

P. expansum SDHIs.

SDHIs , -

.

59


60/192

[`p`isfphrk{q rsdjdv}l `bpn| snt k|ifs` M63) 3)?)i`h `l_-vldtrf sfq E198A kds`jj`b^q lgdishi{sfs`q rs` adlehkh`enjhi\ kt-ifsnis{l` kd sf vp^rf c!-!FL

.. Ufpfrivbolw, .V. Ufvohptz ofn .V. Onhfw , , 75, 118

55

Monilia laxa - . In vitro

: () (RF:

>500, EC50s

) carbendazim 37%

() carbendazim. -

carbendazim

benomyl thiophanate-methyl

- diethofencarb

zoxamide. carbendazim

-

,

fludioxonil, iprodione,

fenhexamid, cyprodinil, tebu-

conazole, boscalid pyraclostrobin. ,

carben-

dazim .

-, -

, carbendazim -

E198A

. Eco31I

-

carbendazim -

PCR-RFLP E198A

.

60 16o


61/192

isf Wtldcp_`

[fkhi^ Alshkds}ohrf Argdldh}lYvtjivjet: Nuno. Sfmlhlydw Sfvfthpfreilw . ofn v. Sfpfqfvbol J.

hrfb^rdhq

ntpthnocw ninylyjw ytz >, jrw rctz $DHI qzolytoyrtz ylw BA$F

$=+; G. ml K. K++

D+ 60/30 DC, t xzrizfxqw + qj >+ ilqntzvhje

crf rct uvtr frf{tvbw hnf ylr inf|jevnxl ytz ~nietz xyt fqucpn

M;-#> F., Ytfxlr [., Uxwlnamu V. ml N. Tnrx

l`inhl}rdhq

"=;+ (F=


62/192

hnjnbhi]q hh{sfsdq snt '


63/192

D@ 60/30 Dc, n rtlt`rk{q + kd +@1, [. Yfvfhznt2, V. Uzynpcofw3 ofn N. Tptz4

1. Development Lead Specialty Crops & Potato Fungicides, Development Centre North-East,

Syngenta Agro GmbH

2. Technical & Development Support Manager, Syngenta Hellas AEBE

3. Campaign Manager Specialty Crops, Syngenta Hellas AEBE

4. Registration Supervisor, Regulatory Affairs, Stewardship & Technical support, Syngenta Hellas

AEBE

Dynali 60/30 DC, , (DC) 60 / difenoconazole 30 /

cyflufenamid. difenoconazole (

) . -

, , , , -

, . -

, .

cyflufenamid -

, . cyflufenamid -

,

,

(DMI) . cyflufenamid

, ,

. , -

, .

difenoconazole cyflufenamid Dynali 60/30 DC

,

(Erysiphe necator), (Guignardia bidwellii) (Pseudopezicula tracheiphila syn. Pseudopeziza tracheiphila), -

, -

. difenoconazole,

cyflufenamid.

, -

. ,

,

, , -

. , , -

63


64/192

, , -

. ,

, ,

.

64 16o


65/192

;@ (F;C20"c), ]l` l]n ktifsnis{ln bh` sfl i`s`on-j]kfrf kdb\jnt `phgkn| `rgdldh}l rd dlp}dhq i`h ifodtshi]q i`j-jh]pbdhdq to`_gpnt i`h gdpknifo_nt

. Yfrvntw, . [yfqfybw, J.L. G= ofn #. M+=


66/192

hnjnbhi^ `mhnj{bfrf snt INI#I$MC rsfl `lshkds}ohrf snt odpnln-ro{pnt. Udhp`k`shi\ `onsdj]rk`s` `bpn| rd i`jjh]pbdhdq `kodjhn|,snk\s`q i`h o`s\s`q

S.V. Uukthptz, S. \xfoevl, \. Spjnyxnrbvlw, [. Uunyngbrtw ofn . [cvglwBASF , . 449, . ,

INITIUM ASF -. Initium , ( bc1), -

.

Initium Qo, . Initium

-

, , . Initium -

. -

-

,

.

Initium (Plasmoparaviticola),

(Phytophthora infestans)

, , .

2006-2012

dimethomorph metiram. -

Initium , -

,

.

66 16o


67/192

hdpd|lfrf sfq do_p`rfq snt ktifsnis{lnt @+;* rsf ut-rhnjnb_` sfq `ko]jnt i`h sfl `lshkds}ohrf `rgdldh}l

[. Stzritzvbw1, .[. Sfvfthpfreilw 2, V. Qjtivtz1, . Yfufgfxnpjetz2,

. Syxntz1, N. _fyklilqlyvetz3, . Spjnyxnrbvlw4 ofn S.V. Uukthptz41. , , ,

54124,

2. , , ,

54124,

3. , , -

, 54124,

4. BASF , 48, , 15125,

pyr-aclostrobin , .

.

( 1S), ( 2S) (, -

-3S) pyraclostrobin.

-

pyraclostrobin.

. -

.

-

-

. ,

. -

.

.

. . ,

Aspergillus spp.,

-

. -

Aspergillus spp.

3S. pyraclostrobin

SPAD CCM .

, - pyraclostrobin

67


68/192

. -

,

,

pyraclostrobin.

68 16o


69/192

mhnj{bfrf `onsdjdrk`shi{sfs`q snt @+;* ]l`lsh a`isfphn-jnbhi}l i`h hnjnbhi}l rgdldh}l sfq snk\s`q i`h hdpd|lfrf snt kf-v`lhrkn| p\rfq

. [ofribplw1, [. [yfzvtzpbolw1, . \keqf1, R. Ufpfrivbol1, [. Uunyngbrtw2, .Spjnyxnrbvlw2, _. fvgcvl1 ofn V. fxnpbotw1

1. , . 8, 14561

2. ASF , 449, 15343,

(strobilurins) . - -

. ,

-

(greening effect). pyraclostrobin

.

pyraclostrobin

. ,

pyraclostrobin,

(speck disease)

Pseudomonas syringae pv. tomato, . , -

, pyraclostrobin - (Cucumber mosaic virus, CMV) (Potato

virus Y, PVY)

, toll. -

,

pyraclostrobin,

.

-

pyraclostrobin

(systemic acquired resistance,SAR).

69


70/192

[fkhi^ i`s`onj]kfrf sfq sdup\q r^wfq rd tpnonlhi^ i`jjh]pbdh`k`pntjhn| i`h tonjd_kk`s` ktifsnis{lxl i`s\ sf rtbinkh^

U. _fyklilqutzptw1, R. Tehofw2 ofn ._. Yfuubw1

1. , & ,384 46 . ,

2. Cadmion , . 153, 202 00

(2010, 2011) - . -

, 15 -

. 4

.

Daconil SC (chlorothalonil 50%) 3ml/lt, Teldor WG (fenhexamid 50%) 1.5g/lt,

Signum WG (boscalid 26.7% + pyraclostrobin 6.7%) 1.5g/lt, Switch WG (fludioxonil

25% + cyprodinil 37.5%) 0.5g/lt Ortiva pti SC (azoxystrobin 8% + chlorothalonil

40%) 2.5ml/lt,

. Switch WG Signum WG

Teldor WG Daconil SC -

. -

.

. Daconil SC

(chlorothalonil 50%), -

-

.

70 16o


71/192

A


72/192

acnkf Wtldcp_`

Qtinsnmhinb{lnh Q|ifsdq i`h ru\jdh` Xpnu_kxlYvtjivjet: Nuno. Sfmlhlydw \xbpyfw . ofn Nuno. Sfmlhlydw \xnyxnhnbrrlw .

l`inhl}rdhq

Utvnfow |fvfoylvnxqw ofn ytsnohtrtw nofrylyf futqtrxj~r qzody~r

A


73/192


74/192

Jdldshi{q i`h knphì{q v`pìsfphrk{q i`h `mhnj{bfrf djjflhi}l kf-snmhinb{lxl ònknl}rdxl snt b]lntq A:79/33


75/192

Qnphì{q v`pìsfphrk{q sxl +@751, ,9i`h )3!bnlh_xl i`h do_-p`rf snt ahnvfkhin| kfv`lhrkn| lgdishi{sfs`q rsntq DMI rsfl opn-r`pknrshi{sfs` i`h ùj`snmhinb{ln hi`l{sfs` snt k|ifs` A:79/33


76/192

- . -

cyp51-

mdr

A. parasiticus DMIs,

A. parasiticus -.

76 16o


77/192

o_p`rf sfq `lgdishi{sfs`q rs` adlehkh`enjhi\ ktifsnis{l` rsf kt-insnmhinb{ln hi`l{sfs` i`h utsno`gnb{ln opnr`pknrshi{sfs` snt k|-ifs` F


78/192

o_p`rf sfq `lgdishi{sfs`q rs` adlehkh`enjhi\ ktifsnis{l` rsf kt-insnmhinb{ln hi`l{sfs` i`h utsno`gnb{ln opnr`pknrshi{sfs` snt k|-ifs` F


79/192

X`tsnon_frf i`h ]jdbvnq dt`hrgfr_`q `lshahnshi}l snt dohutshin|khipnah`in| ojfgtrkn| utjjx}l j`v`lhi}l sfq |opnt

R. rfxyfxetz, N. [bggf ofn . \xbpyfw

, , - , 3603 ,

, -

. ,

,

-

.

, , ,

, , . -

.

95% 87% . -

(83,33%) -

(76,58%). Escherichia coli -

. -

(4,04-5,67 log cfu/g).

.

.

79


80/192

Fbcnf Wtldcp_`

njnb_` - njnbhi]q rg]ldhdqYvtjivjet: Sfmlhlydw R~brrtz V. ofn Nu. Sfmlhdyvnf _fyklgfxnpjetz N.

l`inhl}rdhq

Yvtxintvnxqw ylw rtzopjtyninodw fpplptz|efw ofn frbpzxl ylw htrninfodw

tvhbr~xlw ytz nt ylw utnonpt|pv~xlw qj rfrnxq ylw qjpnykbrfw

(E9+7= 68==/ @+;0 ?;>


81/192

Upnrhnphrk{q sfq lntijdnshhi^q `jjfjntv_`q i`h `l\jtrf sfq bnlh-h`i^q npb\lxrfq snt hn| sfq onhihj{vpxrfq kd l`lhrk{ sfq kdjhse\l`q(E//73); 6;;3, ,>)9 =9


82/192

Qdj]sf sfq bdldshi^q o`p`jj`ishi{sfs`q snt hn| sfq onhihj{vpxrfq kdl`lhrk{ sfq kdjhse\l`q (E//73); 6;;3, ,>)9 =9


83/192

l_vldtrf i`h knph`i{q v`p`isfphrk{q h}l sfq nhinb]ldh`q B;-?= (cN!M%, cG!M% i`h c%A) rd noxp}ldq idp`rh\q

N.. vthofw, .\. Sfyxnbrl, N.S. jplhnbrrlw, _. Sy~vd, V.R. Sfydw ofn .R. Uf-

pnhof , , ,

54 124,

Betaflex-iviridae, - . 2006, 2007 2009

166 12 -

. Betaflexivi-

ridae. , RT-PCR

(RdRp) betaflexi-. -

-

CNRMV

(Cherry necrotic rusty mottle virus), CGRMV (Cherry green ring mottle virus) CVA

(Cherry virus A). -

PCR -

CNRMV, CGRMV CVA.

CNRMV (64/166), CVA (44/166) CGRMV (21/166). -

RT-PCR

.

.

83


84/192

Qnphì{q v`pìsfphrk{q i`h kdj]sf sfq bdldshi^q o`p`jjìshi{sfs`qsnt hn|-1 sfq khipni`po_`q sfq idp`rh\q (L;;3 +099@ =9


85/192

hdpd|lfrf sfq dohfkhnjnb_`q i`h v`p`isfphrk{q h}l snt b]lntqc9=9


86/192

`rg]ldh` snt i_sphlnt i`pntjh\rk`snq sxl u|jjxl sfq snk\s`q rsfl`l`snjhi^ Qdrnbdh`i^ Pdi\lf: U`gnb{lnh hn_, h\nrf, mdlhrs]q i`hv`p`isfphrshi\ kds\nrfq

T._. Yfufhnbrrlw1, . Yfvfxojzutzptw2 ofn V.R. Sfydw31. , .. 22016, 1516,

2. , , 24500,

3. , , ,

.. 269, 54 124,

2005-2012, Begomovirus -Bemisia tabaciGennadius - .

, , , ,

,

8000 4500 -

,

Q 3000 . , -

TYLCV TYLCSV -

B Q B. tabaci

. -

TYLCV 94,5%, TYLCSV 5,5% .

, TYLCV . -

, , -

Q B. tabaci,

, TYLCV .

TYLCV 49 15

, .

TYLCV

TYLCSV. , Hamiltonellasp.

TYLCV.

86 16o


87/192

o`pntr_` snt hn| sfq sphrs]sr`q sxl drodphndh}l rsf p^sf i`hdu`pknb^ kdg{xl `l_vldtrfq snt hn|

M. $+1, . \xeotz1, A. ';?1, H. A1, A. Kfvfhnbrrl1, A. G+?+1,

K. M+?


88/192

H sphrs]sr` rsfl U. . pbnj_`q, s` ld}sdp` dnk]l`

. dqtz1, S. [ufrt1, R. Ufrw1, . \quvfw1, Y. Stzyvcyxlw2, . \keqf3, I. Mf-

pfrivbol3 ofn _. fvgcvl3

1. / & , . & / , 21100

2. , 2, 15123

3. , , , .

8, 14561

, 2000, - , ,

.

, , -

,

. ,

, , -

. -

1994. ,

19

, , 320, 190 150 . , -

, , . ,

3,3 ,

12

, 37, 48 195 ,

. ,

-

2011 . -

, -

365/2002 ( 307) 121285/665/5-9-2006,

-

. , 2010, 2011 2012

3.400 .

, ,

-

.

88 16o


89/192

Tpnjnbhi^ `l_vldtrf i`h knph`i^ s`tsnon_frf h}l sxl wtv`lg}l

N.S. _fyklgfxnpjetz1, . nfotzrydw1,2 ofn [. [yfzvnfrw1

1. , , -

, 75, , 118 55 2. ,

, 34, 16672 ,

. - , 2011 2012, - ,

, , . -

300 , (Phaseolus vulgaris L.)

[Vigna unguiculata (L.) Walp] (Ph. coccineus L.). -

(ELISA) Cucumber mosaic virus (CMV) Bean

common mosaic virus (BCMV) ,

( 38% 8%, )

(44% 57%), / (23% 7%). Alfalfa mosaic virus

(AMV) / (40%)

(4,6%). Bean yellow mosaic virus (BYMV)

(2%) (3%), Bean common mo-

saic necrosis virus (BCMNV) . O

Bean leafroll virus (BLRV) . , (Lens culi-naris Medik.) AMV, BLRV, Pea enation mosaic virus

(PEMV) Pea seed-borne mosaic virus (PSbMV),

CMV (


90/192

l`sf Wtldcp_`

jjfjdo_p`rf Sdlhrs^ ~ U`gnb{lnt Yvtjivjet: Xqynql Sfmlhdyvnf Szvnfotutptz Y. ofn v. Tngnjvbytw R.

l`inhl}rdhq

X ql|frnxqw xehlxlw jric|jyfn rf c|jn mjynod jueivfxl xyl qtpzxqfynoylyf

ytz ntjnitw "8=+=8 ,/; ?;8xylr 8=+7+ ,/7=2+6+7+

Vwpw`oj e., Swvtr K., Usrwn ., thwwu V., \rtwm`mj [., Stmvlnlwj R.

ml S. Snpwcgju

Nyjvpthjw uv~yjrjw qj vpt ofyfxytpcf ytz ql|frnxqt ylw xehlxlw #A

qutvtr rf zutxylvestzr ylr qpzrxl fu ytr n ylw jzpthnbw ylw ifqfxolrnbw

(">6 98 ?;>;

Yjvfnycv~ jsfoveg~xl fpplpjunivbxj~r qjyfs uv~yjrr ytz nt

ytz q~xfot ytz ofn ytqbyfw ofn qjpcyl ylw fryeivfxlw ytz {zyt

xyl qpzrxl

Uklrxg`mju U.U. ml R._. Tllht`wru

I7 ?=;8 qjyfhvf{d ytz "/978 68


91/192

T kfv`lhrk{q r_bfrfq dl]vds`h l` ]vdh gdshi^ do_p`rf rsf knjtrk`-shi{sfs` snt hndhn|q 6;);6 :7,3 ;


92/192

sdp{jnbdq opxsdldq kd p{jn i`s`rsnj]` snt kfv`lhrkn| sfq r_bfrfq!NA konpn|l l` tonrsfp_mntl sfl k{jtlrf `o{ snl h{ sfq dtjnbh\qsfq `k`riflh\q (3


93/192

Qdj]sf sfq opnr`pknb^q snt hn| Y sfq o`s\s`q (6;);6 =9


94/192

Udp`hs]px dm`ip_axrf `jjfjdohp\rdxl kds`m| opxsdyl}l snt hn|snt kxr`yin| snt i`h snk\s`q i`h kdj]sf sfq `ls_p`rfq sntutsn| rsf k{jtlrf

U.U. Ufmntzibolw1,2 ofn R._. Tngnjvbytw11. , ,

, , 73100

2. , , ,

.. 269, 54124

pepino (Pepino mosaic virus, PepMV) Potexvirus Solanum muricatum(pepino) 1980. -

. PepMV -

-

,

- . ,

( 70 [Hsc70] )

p25 , , -

. -

in vivo in vitro

.

94 16o


95/192

I =;96kds`bp`u^ snt 76 6:)+ =9


96/192

K]i`sf Wtldcp_`

Tjnijfpxk]lf lshkds}ohrf rgdldh}lYvtjivjet: Nu. Sfmlhdyvnf Tfhtuil J. ofn Tcoytvfw \kbqtw [.

hr^bfrf

Nueivfxl ytz jqgtpnfxqt y~r pf|frtotqnor jnir xylr frt|d

ofn fryt|d ytz jqgtpetz xynw fxmcrjnjw

Srxy`mju Q.

l`inhl}rdhq

Ujpcyl ylw frmjoynoylyfw y~r infhtrninfor {zyr ytz zgvnietz ytqbyfw

CL3731 utz zujvjo{vbktzr yt htreint 64qjyb fu uvtxgtpd

qj yt gfoydvnt ".


97/192

o_p`rf snt dkanjh`rkn| sxl j`v`lhi}l dh}l rsfl `lnv^ i`h`lsnv^ snt dkanj_nt rshq `rg]ldhdq

Q. Stz{bolw

AGRIS .., , 59032 72200,

(, , ) , -

. -

, ,

.

-

,

. ( ),

,

-

.

, .

, ,

.

97


98/192

Qdj]sf sfq `lgdishi{sfs`q sxl h`bnlhh`i}l uts}l snt taph_nt sn-k\s`q cL'3731 ont todpdiup\entl sn bnl_hn //:;


99/192

Qdj]sf `mhnj{bfrfq sfq do_p`rfq h`unpdshi}l utrhi}l ntrh}lrsfl `lsnv^ sfq pnh\q rd v`kfj]q gdpknip`r_dq

Y. vthtil, . cppf ofn . Yfryjpeilw

, , , .. 38, 59 035

-, 2011.

,

.

-

, -, , ,

,

. -

(-

) , 8 3 ,

-

.

: )

, ) ,

) .

.

,

- ,

. -

,

.

99


100/192

ns`lhi\ lfk`sxnis{l` rsn oj`_rhn sfq njnijfpxk]lfq h`vd_phrfqsxl phe{inkaxl lfk`sx}l M36,6/@ .

. Vybppl ofn !. Ujroexthptz-[uzvtil

, , , 54124

(RKN; Meloidogyne spp.), , -

, -

, .

,

(Regulation 2009/1107/EU & 2009/128/EU),

. ,

,

. ,

, -

. -

, .

, -

.

.

, ,

, .

-

Meloidogyne spp.

-

. -

in vivo;

(AchE) in vitro.

-

,

.

, -

.

100 16o


101/192

o_p`rf h u{pxl utshi}l divtjhrk\sxl i h sfq vhsnr\lfq rd kn-j|lrdhq vptr`lg]knt kd sn hndh]q c09@:);0


102/192

o_p`rf snt hn| snt kxr`yin| sfq `bbntph\q (cM%) i`h snt *-; (B#H) rd onrnshi\ i`h onhnshi\ v`p`isfphrshi\ dkonpd|rh-kxl i`po}l snk\s`q

M. Sfpthdvtz1,2, . fvgcvl1, . fxnpbotw1 ofn L.A.%;;21. , . . 8, 14561 , -

2. Plant Science Laboratory,Cranfield University, College Road, Bedfordshire, MK43 0AL, United

Kingdom

(Cucumbermosaic virus, CMV) benzothiadiazole (BTH, 50 WG, 50 mg/L), - , -

(F1 )

. O CMV

(CMV+BTH, BTH,

) ,

.

, -

, , -

.

BTH ,

. , ,

, BT,

-, -

. BTH,

CMV, -

,

,

. BTH -

CMV -

.

102 16o


103/192

mhnj{bfrf sfq onsdjdrk`shi{sfs`q hò]p`rsxl oj`rshi}l u|j-jxl i\jtwfq rd du`pknb^ fjhnònj|k`lrfq rd rtlt`rk{ kd khr^sfq rtlhrs}kdlfq {rfq ònjtk`lshi}l d\untq rsfl `lshkds}ohrf

dùnbdl}l o`gnb{lxlY.Y. ry~retz1, R. nfrrfot2 ofn N.S. \kbqtw1

1. ,

2. , ,

, 75, 118 55

- ORGASUN (4 6 - ) .

-

. CONDOR:

20 lt/. (100%) , 10 lt/. (50) . VAPAM: 55 lt/. (100%)

, 27 lt/. (50%) .CHLOROPICRINE: Pic (67:33 v/v) lt/

(100%) , lt/ (50%) .

-

. 2011, -

2011 2012 (

). -: ,

Meloidogyne.

Fusarium oxysporum,

Fusarium oxysporum f.sp. radicis lycopersici -

.

Pyrenochaeta lycopersici.

: -

.

( 8.46-9.33). -

-

.

Fusarium oxysporum -

Fusarium oxysporum f.sp.

radicis lycopersici Pyrenochaeta lycopersici -

orgasun.

103


104/192

Fusarium

oxysporum f.sp. radicis lycopersici( 2012) 16%

.

104 16o


105/192

o_p`rf hg]phxl dj`_xl o]lsd pxk`shi}l uts}l rsfl ktifjh`i^`l\ostmf i`h sfl aj\rsfrf inlh_xl sxl kti^sxl +33


106/192

Llc]i`sf Wtldcp_`

hnjnbhi^ lshkds}ohrf rgdldh}lYvtjivjet: Xqty. Sfmlhdyvnf \kfgcppf-Sp~rbvl S.

ofn Sfmlhlydw Kjpnxxfvetz .

l`inhl}rdhq

Nhofybxyfxl y~r ufmthr~r ">7+ 9>7=08;66

8/8


107/192

bi`s\rs`rf sxl o`gnb{lxl kti^sxl


108/192

mhnj{bfrf jjflhi}l `onknl}rdxl kti^sxl snt b]lntq #9+06-,9)xq ahnjnbhi}l o`p`b{lsxl `lshkds}ohrfq d`unbdl}l utsn-o`gnb{lxl kti^sxl

.. Uubvifw, Y. hhjptutptz, _. Nzxyvfyetz, . \kerthptz, . Utzytpl, .\xfrfoyxeitz, . Yfpbytw ofn [. [yj{frtz

, 57400

Verticillium dahliae, Rhizoctoniasolani, Pythium ultimum Sclerotinia sclerotiorum

. -

, DNA (ITS1 ITS2

), 32 Trichoderma.

-

in vitro . -

. ,

, -

, Trichoderma asperellum Trichoderma

harzianum,

. Trichoderma viride

, -

. -

( ,

)

( , -

R. solani). , Trichoderma harzianum2

Trichoderma viride B4 -

. , -

in planta .

108 16o


109/192

mhnj{bfrf i`h kdj]sf snt kfv`lhrkn| p\rfq snt `ls`bxlhrshin| a`-isfp_nt )*)+3


110/192

onk{lxrf l]xl ahno`p`b{lsxl lshkds}ohrfq sfq r^wfq phe}l i`hj`hkn| sfq snk\s`q ont opni`jd_s`h `o{ sn k|ifs` F


111/192

mhnj{bfrf kdg{xl `onk{lxrfq ktino`p`r_sxl sxl rijfpxs_xlsnt utsno`gnb{lnt `rink|ifs` "+396;) :+396;69


112/192


113/192

YNZRTP`NR[

NRSXVXZ^PUNVaVNZ[RaV


114/192


115/192

LNTRTJVHZMQLRLW LVJHWNLW / O"#E!"

Up}sf Wdhp\ Lhinlnbp`ufk]lxl Lpb`rh}l

Qtifsnjnbhi]q-Upni`ptxshi]q-Nnjnbhi]q i`h Qf U`p`rhshi]q Hrg]ldhdq -

Rfk`sxcnjnb_`


Nueivfxl ylw pyjvrfve~xlw xylr ujvnjoynoylyf fryntsjni~ynor tzxnr xj ofvutw uvt-

xgjgplqcr~r uvcqr~r foynrnie~r}pncgrx ., Stopnj S., Strfnpcgju .[., Uhpmcvrfnrx-[sxtrgj X. ml . Mlopwc-

gju

Uzolytpthnocw fxmcrjnjw ytz lpefrmtz xylr Y.N. vbqfw ylr injyef 2010-2011pvwvl`gju .i.

ujrjvhtutelxl ytz htrnietz ''/A ('?= A) xyt qolyf '/;=33553>6 +253+/ ofn injvjrlxlytz vptz ytz xyl {zxntpthef ofn ufmthcrjnf ytz qolyfpw|pl`gj . ml M.R. \vlwvlfl`ppju

vdhtvl ofn fovngdw yfzytutelxl y~r jninor qtv{r ytz {zytufmthrtz qolyf F>6

qj yl |vdxl ylw z}lpdw infovnynodw nofrylyfw ofqup~r ydslw (H#M) frbpzxlfrutzptw R., Uficxlw Y. ofn J. \xfzybvlw

Utvnfod ofn {zytufmtpthnod injvjrlxl ytz vptz ytz vzmqnxynot htrnietz ytz ijzyj-vthjrtw qjyfgtpnxqt AL+/A xyt qzotytsnothrt qolyf A< +;87+;3>6 +253+/fu crfr rct sjrnxyd, yt knkbrnt $85+7>6 /5+/+1730853>6Tfrsgj .T., K+


116/192

Yvyl frf{tvb injmrw olpei~xlw y~r {pp~r ytz {ternof "28/73 =2/892;+6Stxrtxvp`mj V., nhql`gju \. ml S. Snpwcgju

116 16o


117/192

Yvyl frf{tvb ytz nt ylw pjofrxlw y~r rjv~r ylw qtp|fw (M'C') ofn ytz nt ytz oe-yvnrtz q~xfot ylw {fxtpnbw (B*M') xylr NppbifTwru T., Xtypcgrx _.. ml V.R. Swbu

Yvyl frf{tvb jrw xzhhjrtw qj ytr BL#' nt xj yvef jeil }z|frmrTwru T., \vl`nwu R.Q. ml V.R. Swbu

P ufvtzxef ytz nt ylw yvnxycyxfw xylr Y.N. _fre~rUnpgt`mj N., Mlm`trx N. ml Y. rflw`mju

Utvnfod fre|rjzxl ytz nt ylw fqucptz (G'A) ofn ytz nt utz x|jyekjyfn qj yl gtmve~xlytz otvqt ytz #> + ("MA)_tlvwrytrx U., Ypwhncgju R.[., Spawju, T., \v`nwu M. ml V. R|`pprx

Sb}nqt y~r xuv~r, ql ufvfxnynod fxmcrjnf ytz lpefrmtzpvwvl`gju A.i.

Noyeqlxl y~r junuyxj~r fu z}lpcw xzhojryvxjnw yvtutx{fnvnot ktrytw xj tu~vrjwvtifonrnbw ofn ifqfxolrnbw xylr Pqfmef, |vlxnqtutnryfw ufmlynotw ijnhqfytpduyjwMtrfrgj Y. ml . Ypwhncgju

117


118/192

Kd|sdpf Wdhp\ Lhinlnbp`ufk]lxl Lpb`rh}l

[fkhi^, Ihnjnbhi^ i`h Tjnijfpxk]lf Hlshkds}ohrf Hrgdldh}l

_PURSP JV\RUN\aYR[P

ntpthnod ivbxl ofn jzfnxmlxef bhvn~r uplmzxqr ytz qolyf B8=;=3< 37/;/+xyt rctqzolytoyrt ylw tqbifw y~r ufvjqutinxyr ylw f{zivthtrbxlw ytz lpjoyvnot tsctw >-;+hnrmu Q. ml Strfnpcgju .[.

Nzfnxmlxef futqtrxj~r y~r qzody~r A< 731/;ofn A< +;87+;3>


119/192

sntphlxl ylw jueivfxlw Npplrnor futqtrxj~r ytz hcrtzw %;328/;6+xyf gntpthnob|fvfoylvnxynob frbuyzslw {zyr fvrnfw (A;873+ 6/5+78+;9+)Us`tgu .., Yapwvrx Y., Mwvlrx U., Upgt`u _., Urxwrnj ., \vpmwvcgrx ., Yn`wru .

ml [. [why`prx

utqr~xl ofn qtvnfow |fvfoylvnxqw jun{zynor kzqr fqucptz ofn fsntphlxl ylwfryfh~rnxynodw ytzw ivbxlw jrfryetr qzody~r ytz hcrtzw A<


120/192

[zhovnynod qjpcyl frbuyzslw y~r {zytufmthr~r qzody~r xj izt xzxydqfyf ofppncvhjnfwmjvqtolunfodw ytqbyfwYsgrsrnrx U.

jn{tvnod |vdxl futpzqfrynor jib{tzw hnf ylr frynqjyunxl jif{thjrr ufmthr~rxyf tu~vtolujzynob (LIFE 2008 - $>


121/192

Up}sf Wdhp\ Lhinlnbp`ufk]lxl Lpb`rh}l

Qtifsnjnbhi]q-Upni`ptxshi]q-Nnjnbhi]q i`h Qf U`p`rhshi]q Hrg]ldhdq -

Rfk`sxcnjnb_`


o_p`rf sfq jsdpl`p_xrfq rsfl odphdishi{sfs` `lshnmdhxshi}l nt-rh}l rd i`pon|q opnradajfk]lxl op]klxl ishlh_xl

. rfpeitz1, S. Sfvfqfrpl1, .[. Sfvfthpfreilw3, X. Ujroexxthptz-[uz-

vtil2 ofn . nfqfryeilw1

1. , , , 54124 , 2. , , , 54124 ,

3. , , , 54124 ,

Alternaria alternata - .

-

( )

. CPPU (forchlorfenuron),

, -

-

.

Alternaria alternata -

forchlorfenuron.

FRAP

DPPH, -

(HPLC). -

-

CPPU.

forchlorfenuron -

50%. -

.


122/192

Qtifsnjnbhi]q `rg]ldhdq snt fj_`lgnt rsfl U.. p\k`q sfl hds_`2010-2011

.I. rfxyfxnbilw

... , , 5 . -/

(2010-2011) , .

, , ,

, , , , .

, 2011, -

15-20%, 2011 -

.

(-

, , ), 2011,

2011 2010 -

. -

(, , )

, - -

90%. 2011 50-60%, (

) . -

2 , -

, .

122 16o


123/192

odldpbnon_frf snt bnlh_nt %,%A (%=; A) rsn k|ifs` %9;+33


124/192

Jp^bnpf i`h `ipha^q s`tsnon_frf sxl dhhi}l knpu}l snt utsno`-gnb{lnt k|ifs` F


125/192

Qnph`i^ i`h utsno`gnjnbhi^ hdpd|lfrf snt p{jnt snt ptgkhrshin|bnlh_nt snt dtsdpnbdln|q kds`anjhrkn| A+L)A rsn ktinsnmhinb{lnk|ifs` A:79/33


126/192

Up}sf `l`unp\ snt k|ifs` D)769;0 6;0+63)xq o`gnb{lnt ontopni`jd_ l]ipxrf aj`rs}l rsfl `ishlhh\

Q. Q~qeilw ofn N. Nsfifoyptz

.... , , , , .. 57400, -

. .

-

, . 2009,

( Hayward )

(. ),

- .

-

, .

potato dextrose agar (PDA)

ITS-5.8S rDNA-ITS2 Elongation Factor 1-alpha

CBS Fungal Biodiversity Centre, Identification Service (Utrecht, Netherlands)

Diaporthe neotheicola A.J.L. Phillips & J.M. Santos.

Koch, ,

. .

,

10 30C, 20 25C.

D. neotheicola

, .

126 16o


127/192

Qds`anjnkhi^ `l\jtrf sxl utj}l 1 i`h 2 snt k|ifs` %9;+33


128/192

X`tsnon_frf i`h rtvl{sfs` o`gnb{lxl `hs_xl opnrtjjdishi}l i`h kd-s`rtjjdishi}l r^wdxl rd i`pon|q pnh\q

[. S~rxyfryertz ofn .[. Sfvfthpfreilw

, , ,.. 269, .. 54124,

, , , . .

. -

,

.

- -

.

2011 (- ),

. . -

, -

. 5

3 .

Aspergillus spp. (45.9 %)

Penicillium spp. (40 %). -

Botrytis cinerea (70.8 %) Pilidiella granati(15.4 %). Aspergillus spp.

(RFLP,

Restriction Fragment Length Polymorphism) A. niger 65.5 % .

tubigensis 34.5 %. Penicillium spp.

ITS1 ITS2

DNA. Penicillium spp., -

P. adametzioides

P. brevicompactum 50% . -

, P. adametzioides. -

P. adametzioides P. brevicompactum

, . ,

2012,

.

128 16o


129/192

hdpd|lfrf sfq dto\gdh`q i`jjhdpbn|kdlxl rnj`lx}l i`h djh\q rsn%9;+33


130/192

Up}sf `l`unp\ sfq r^wfq u|jjxl 06?. opni`jn|kdlfq `o{snl `rink|ifs` N6,/0;6) 706+


131/192

Up}sf l`unp\ hdgl}q ifj_xrfq sxl u|jjxl snt un_lhi` 06?;06709):;opni`jn|kdlfq `o{ snl `rink|ifs` )9)+66;0@9


132/192

Up}sf l`unp\ p{hlfq r^wfq unhlhindh}l 06?i`h &):0/;6). opni`jn|kdlfq `o{ snl `rink|ifs` N)3);0))3) =96:rsfl jj\`

N.S. Tnhtszhobolw1, R.. Yfuf~brrtz2, N.. Ufvobolw3, N.N. ^vfhotpl4 ofnU.. \ufw2

1. , , -

, 71003,

2. , , -

, , 15701

3. , ,

, 71004,

4. , , -

Phoenix Washingtonia -. ,

, ,

, , -

-

. ,

( ), - ( ITS1-5.8S-ITS2

18S, BLAST) (

) ,

Nalanthamala vermoesenii(Biourge) Schroers (. Penicillium vermoesenii, Glio-

cladium vermoesenii). P. ca-

nariensis, P. theophrasti W. filifera

. W. filifera

. N. vermoesenii -

. -

N. vermoesenii .

,

, Rhynchophorus ferrugineus.

132 16o


133/192

Qnph`i{q v`p`isfphrk{q onknl}rdxl snt o`gnb{lnt k|ifs` D6;0-:;96).

O.. Vfotutptz1, _. Yjvpcvtz2 ofn [. nfqfrydw1

1. , , 570 06,

2. , 1, 546 26,

23 (Pinus brutia L.) - .. Dothistroma spp.

(Dothistroma Needle Band Disease).

,

: 1. ITS

ITS1 ITS4

-

, 2. ITS ITS1

ITS4 RFLPs (Restriction Fragment Length Poly-

morphisms) 3. 1-1, 1-2 -

, 4.

DMS A.

23 Dothistroma septosporum (Dorog.)

M. Mopelet , Dothistroma piniHulbary

B . -

, Dothistroma septosporum Dothistroma pini, -

-

.

133


134/192

Wtvl{sfs` i`h v`p`isfphrk{q sxl rtedtishi}l s|oxl MA#1-1 i`h MA#1-2 rsnl k|ifs` c9@706+;9) 7)9):;+)

O.. Vfotutptz1, _. Yjvpcvtz2, . vtkfw3, . Ulyxfobol4 ofn [. nfqfrydw1

1. , , 570 06,

2. , 1, 546 26

3. , , A

, .. 104, .. 541 24,

4. , , 491 00

Cryphonectria parasitica

-

1963. 707

C. parasitica

-

. -

PDA DNA

(PCR) 1,649 kb (MAT-1) 594

bp (MAT-2). 707 655

1-1, 52 1-2 1 -

1-1,2. 1-1 1-2 92,6% 7,35% , -

627 11 -

. ,

.

134 16o


135/192

Sfp\lrdhq dlptjj_xl `ktb`jh\q ont nud_jnls`h rsn k|ifs`B6;9@6:70)9) ,6;0,)

R._. Ztqutw1 ofn .R. "tqutz2

1. ()

2. ,

- 2011 (, . ) -

.

. . -

(40-70%) . -

-

. . -

. H ,

, -

.

. B.

dothidea (Moug.: Fr.) Ces. & De Not. Neo-fusicoccum mediterraneumCrous, M.J. Wingf. & A.J.L. Phillips

, 23-29 x 4,5-5,0 m. H -

-

.

. -

B. dothidea .

135


136/192

fj_xrf sxl u|jjxl snt I?


137/192

YZXSJZ]a\RSN[ J[QNVNRN[

Up}sf `l`unp\ sfq opnranj^q |n ans`lhi}l onhihjh}l D);


138/192

:


139/192

Upnranj^ uts}l i`ponteh\q, odonlh\q i`h `bbntph\q `o{ utsno`gn-b{ln a`is^phn snt b]lntq A+,6=69)?rsfl jj\`

U.S. _tpcgf, Y.N. pzrw ofn _.. Sfvb{pf

, , ,. 8, 145 61

- 2012, , - ,

, / ,

, , -

. ,

. -

10% 100% -

. ,

.

, , , ,

,

Acidovorax.

16S rDNA A. valerianellae, PCR

A. citrulli.

.

-

Acidovorax, .

139


140/192

Up}sf `lìn_lxrf opnranj^q uts}l oj`s|utjjnt a`rhjhin| ò{sn utsno`gnb{ln aìs^phn :


141/192

RXTXRSN[ J[QNVNRN[

lsnohrk{q snt hn| sfq sphrs]sr`q sxl drodphndh}l rsfl U. . P`-ixl_`q

.. vlhtvbotz1, Y.. _vnxyt{npbotw1, Y. Stzyvcyxlw2, . \keqf3 ofn _. fvgcvl3

1. & .. , , 2 .

-, 23100

2. , 2, 15123

3. , , .

8, 14561

(Citrus tristeza virus, CTV, Clos-terovirus, Closteroviridae) .

2007 ,

Clemenpons Carrizo citrange

2001. , 939 ,

( 10%), .

2009

. 2011

. , 365/2002 (

307) 121285/665/5-9-2006,

, 21.163 ,

31 ,

, -

. ,

,

, -

, (, , -) .

.

. ,

,

-

.

141


142/192

Wtvl{sfs` dku\lhrfq h`u{pxl o`gnb{lxl rd l]ntq noxp}ldq pn-`ihlh\q

S.N. Nzmzqetz, Y.. Yfuud, T. Tytw, .Q. Sfyxnbrl, [. ofgfpcof ofn V.R. Sfydw

, , ,54 124,

, (), .

, , ,

(Plum pox virus, PPV),

(Apple chlorotic leaf spot virus, ACLSV),

(Prunus necrotic

ringspot virus, PNRSV), (Prune dwarf virus,

PDV), (Peach latent mosaic

viroid, PLMVd) -

(European stone fruit yellows phytoplasma, ESFY).

2010 2011 -

.

360 -

, . -

PCR. PLMVd (66,66%) PNRSV (16,1%),

PPV (0,83%), ACLSV (1,11%), PDV (1,38%),

ESFY (0,55%), .

-

.

142 16o


143/192

Up}sf `l`unp\ snt hn| sfq dtjnbh\q sfq `k`riflh\q rd `ktb`jh\rsfl jj\` rsn oj`_rhn utsnzbdhnlnkhin| dj]bvnt

U. Sfurl1, N.. sfvpd2, Y. Stzyvcyxlw2, V. Vnotptzibolw2, Y. vthtil3 ofn

U.. Uujvubyl41. 5, 151 26

2. ...&., , 151 23

3. , , ,

59 035 4 26, 181 22

, ,

(Plum pox potyvirus, PPV). PPV

(Prunus dulcis, . Prunus amygdalus Batsch) , , ,

. , .

-

()

PPV DAS-ELISA -

. 2010-2012 PPV

, .

(, , ).

(Immunocapture

RT-PCR, IC-RT-PCR) DAS-ELISA, -

.

, .

,

.

143


144/192

Z`hlnstohi^ i`h jdhsntpbhi^ l\jtrf snt "E!!A#Erd uts\ N+6;));)*)+


145/192

Up}sf `l`unp\ snt hn| sfq jd|i`lrfq sxl ld|pxl sfq knj{v`q(M%c%) i`h snt hn| snt i_sphlnt kxr`yin| sfq u`rnjh\q (BM%) rsfljj\`

T. Tytw, _.. Xv{freitz ofn V.R. Sfydw , , ,

54 124,

Potyvirus - 146 , . - 2011 -

.

RT-PCR

Potyvirus. , -

Poty-. , Malva sylvestris

() Cassia . -

PCR

BLASTn -

NCBI. 97%

M. sylvestris

(Malva vein clearing virus MCVC) 91% o Cassia

(Bean yellowmosaic virus BYMV).

MVCV BYMV Cassia.

145


146/192

Up}sf `l`unp\ dl{q rtbbdln|q kd snl BL!% hn| rd sp_` d_f wtv`l-g}l

T. Tytw1, R.Q. \xnbpyfw2 ofn V.R. Sfydw1

1. , , ,54 124,

2. , , , 54 124,

(Bean leafroll virus - BLRV) Luteovirus

. 2011 -

Luteo- (Vicia sativa L. subsp. sativa) (Vicia

ervilia (L.) Willd.) ,

(Medicago sativa L. subsp. sativa) .

, , -

RT-PCR BLRV

(). 391 -

. -

BLASTn

98-99% (HE601635),

(HE601636) (HE601637) BLRV. - Luteo-,

.

, ,

.

, BLRV

.

146 16o


147/192

.

. M, . .

/ & , . & / , ,

73103

, , - 2000, Lane late.

20 ,

Carrizo citrange,

1994. , -

. - 2000-2002 4000

-

. 2011

9.000 ( )

,

... & .

, 140 -

( , -

). , ,

. ,

Lane late, Washington navel New hall.

.

147


148/192

Qnph`i^ `l_vldtrf snt hn| sfq `ko]jnt (G%A) i`h snt hn| ont rvds_-eds`h kd sf angp_xrf snt inpkn| snt !


149/192

hshnjnb_` his]pnt rd i`jjh]pbdhdq injnitlgndh}l sfq v}p`q k`q

_.. Xv{freitz1, T.. Yfufhnbrrlw2, T. Tytw1, . lqlyvetz1, . nthcrtzw1,

.R. Ufpnhof1 ofn V.R Sfydw1

1. , , ,54 124,

2. , .. 22016, 1516,

- . - ,

(BPYV), -

(CYSDV)

(CABYV). BPYV, CYSDV

CABYV . BPYV CYSDV -

Trialeurodes vaporariorum Bemisia tabaci, , CABYV

Aphis gossypii Myzus persicae. 2011-2012

, -

.

RT-PCR, , real-time PCR.

128 4 (, ,

, ), 34 6 241 .

. ,

CYSDV 33,7%,

BPYV CABYV 26,9% 21,35% -

. , , CABYV

58,97%, CYSDV 46,15%, BPYV

. -

-

.

CYSDV CABYV .

149


150/192

l_vldtrf i`h v`p`isfphrk{q snt hn| sfq sphrs]sr`q sxl drodphndh}lrsfl |opn kd knph`i]q sdvlhi]q

T._. Yfufhnbrrlw, . Szvnfot ofn Q. Sfufvd-Pxff

, 1516, ,

(Citrus tristeza virus, CTV), 1968, - (Citrus au-

rantifolia), ELISA.

CTV , -

,

. -

-

(Real-Time TaqMan RT-PCR)

-

.

, ,

RT-PCR.

, 12-15

LNAs (Locked Nu-cleic Acids)

, .

, ,

.

150 16o


151/192

l\ostmf kdg{nt op`bk`shin| vp{lnt `ls_rspnufq kds`bp`u^q-`jt-rhxs^q `ls_p`rfq sfq onjtkdp\rfq (! # !#-c!) rd ]l` n-ihk`rshi{ rxj^l` bh` sfl `l_vldtrf i`h onrnshinon_frf `onknl}rdxl

snt EMD% rd h\unpntq mdlhrs]qY.. Yfuud1, _.R. gfw2, S.N. Nzmzqetz1 ofn V.R. Sfydw1

1. , , ,

54 124,

2. , ,

, 54 124,

(Eggplant mottled dwarf

virus, EMDV), 1969.

-

-

.

-

(Real Time qRT-

PCR) ,

EMDV -.

210

. -

RNA RT-PCR. - RT-PCR

RNA .

(amplification efficiency) 96,9%.

2x108-10 RNA.

,

, , , , , .

-

- , - .

151


152/192

T h{q sfq sphrs]sr`q sxl drodphndh}l rsfl U. . nphlg_`q

. ^pvtw1, . \keqf2 ofn _. fvgcvl2

1. & .. 36, 20100 -

2. , . 8, 14561 ,

- , 190, 90 - . 2011,

,

,

.

, , , -

. , 637

,

, 300

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, 7.655 , ,

, 365/2002 ( 307), 665 /2006 (

14167) . 2147/5192 (155 ). -

,

, .

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,

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.

152 16o


153/192

l\ostmf kdg{nt fkh-drsh`rk]lfq !#-c! bh` sfl l_vldtrf snt j`l-g\lnls` hn| sfq adphinih\q (A79+6; 3);; =9


154/192

VPUJ\aXTXRJ SJR UP YJZJ[R\RSN[ J[QNVNRN[

Th lfk`s}dhq xq ahnd_isdq sfq tbd_`q snt d\untq rd odhp`k`shi^i`jjh]pbdh` fj_`lgnt

N. Sfvfrfxybxl1, . \vnfryf{zppeilw2, T. Xnotrqtz3, V. Ubryktw4, . Ubrtw5

ofn R. S~rxyfryertz4

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,

2. , &

,

3. , & -

, , 4. , , ,

5. , , ,

1000m2

, . 16

40m2 , 8 5% 8

1%. , , -

-

8 , 4 5% 4 1%,

4-5 cm, 70 cm 20cm .

8 . 2 -

, oxyfluorfen ,

quizalofop-p-ethyl .

8 , 4

4 , 2 5% 2

1%. 8 , 5% 1%

5% 1% . - 0-10cm. -

, cm3,

/ -

, , -

STATISTICA ver 7.0.

- (split-split-

plot) -

(p


155/192

h`vxphrk{q exls`l}l/ldip}l `tb}l sxl itrsnb{lxl lfk`sx}lsfq o`s\s`q kd sf vp^rf


156/192

\whkn sxl ro{pxl, kf o`p`rhshi^ `rg]ldh` snt fj_`lgnt

.R. rfxyfxnbilw

... , , 5 . -/

, , , ()

-

. ,

( -

). -

2011,

.

R6-R7. ,

, , -

- .

-

.

,

.

-.

156 16o


157/192

is_kfrf sxl dohos}rdxl o{ twfj]q rtbidlsp}rdhq spnonru`hphin|{enlsnq rd noxp}ldq pn`ihlh\q i`h `k`riflh\q rsfl k`g_`, vpf-rhknonh}ls`q o`gfshin|q dhbk`snj^osdq

Y. vthtil ofn . Yfryjpeilw , ,

, .. 38, 59 035

(3) -

, -

.

3 ,

. 10 3

radiello 9 1 ,

.

3

. 3 26-45 ppb, 31-50 ppb 35-49 ppb

2005, 2006 2007, . 3

( )

( ). 7%

2005, 13% 2006 14% 2007

,

12%, 1% 16% 2005,

2006 2007, , . 3 ,

.

, , -

, .

157


158/192

U`pntr_`rf Kd|sdpfq Wdhp\q Lhinlnbp`ufk]lxl Lpb`rh}l

[fkhi^, Ihnjnbhi^ i`h Tjnijfpxk]lf Hlshkds}ohrf Hrgdldh}l

_PURSP JV\RUN\aYR[P

hnjnbhi^ p\rf i`h dt`hrgfr_` \bphxl ojfgtrk}l snt k|ifs`B6;9@;: +9)rsn l]n ktifsnis{ln sfq nk\`q sxl o`pdkonhrs}lsfq `utpnbnl\rfq snt fjdisphin| nm]nq


159/192

t`hrgfr_` `onknl}rdxl sxl kti^sxl A:79/33


160/192

Qdj]sf sfq do_p`rfq sxl ktifsnis{lxl ont odph]vnls`h rs` o{ajfs`sxl rtridt`rsfp_xl upn|sxl rsntq khipnnpb`lhrkn|q snt d\untq

N.[. Yfufitutptz1, X. Ujroexthptz-[uzvtil1 ofn .. Sfvutkfw2

1. , , , 54124

2. , , 26 & , 41

221

thiabendazole (TBZ), imazalil (IMZ), ortho-phenylphenol(OPP) Penicilium sp. Geotrichum sp.

. -

,

.

-

, -

(50 g/g )

,

.

- ( C), (

) ( FDA), 3, 10, 30, 60 90 .

( )

(DGGE).

, .

,

.

DGGE -

. -

,

-

.

160 16o


161/192

p\rf :;


162/192

!OLEc#$"#M (F@): R]n ktifsnis{ln bh` snl ]jdbvn sntnsp|sf rd `ko]jh, j`v`lhi\, up\ntj` i`h sfq Qnl_jh` rd otpfl{-i`po`

$. %+++1, . K>;+1 ofn ]. $+21. Sumitomo Chemical Company, ltd., Tokyo, Japan

2. Sumitomo Chemical Agro Europe, Lyon, France

PROLECTUSTM

fenpyrazamine (S-2188) Sumitomo Chemical

Co., Ltd., . H fenpyrazamine

(pyrazolinone)

, - Botrytis, Sclerotinia sclerotiorum,

Monilia.

,

, , -

. .

-

, , , -

. PROLECTUSTM

50% fenpyrazamine.

, , . -

.

(PHI) 14 7

( ) 1 . PROLECTUSTM

. PROLECTUSTM

80 120 ./

Botrytis cinerea , .

Monilia Sclerotinia.

, , , PHI.

PROLECTUSTM IPM,

.

162 16o


163/192

o_p`rf snt ktifsnis{lnt @+;* rsf utrhnjnb_` snt rijf-pn| r_snt i`h sfl `lshkds}ohrf `rgdldh}l

R.Q. \xnbpyfw1, .[. Sfvfthpfreilw2, . Yfufgfxnpjetz2, . Spjnyxnrbvlw3, S.V.

Uukthptz3 ofn . Sryxfw31. , , , 54124,

2. , , ,

54124,

3. BASF , 48, , 15125,

2010-11 2

( )

pyraclostrobin . -

pyraclostrobin

.

pyraclostrobin 2

2 .

Fusarium spp.

. -

pyraclostrobin BBCH 39

8,69% . .

pyraclostrobin

(: Tcanopy-Tair).

(2180 kg/ha) 8% 56.2%.

pyraclostrobin.

pyraclostrobin

. -

BBCH 30 BBCH

39, BBCH 39

.

163


164/192

hdpd|lfrf sfq dt`hrgfr_`q `onknl}rdxl snt k|ifs` B6;9@;: +9)`o{ i`jjh]pbdhdq pnh\q rs` ktifsnis{l`


165/192

KRXTXRSP JV\RUN\aYR[P

hnjnbhi^ lshkds}ohrf snt snmhinb{lnt k|ifs` A:79/33


166/192

H nk`hi^ i_lfrf `ls`bxlhrshi}l rsdjdv}l :


167/192

ohjnb^ kf-snmhinb{lxl rsdjdv}l snt k|ifs` A:79/33


168/192

mhnj{bfrf sfq p\rfq phena`isfp_xl ]l`lsh d`unbdl}l utsno`-gnb{lxl kti^sxl =;96

. Ufpf{tvlw ofn .T. Tfhtuil

, ,

(8) , in vitro in planta Fusarium oxysporum f. sp. radicis-lycopersici(Forl) 3 , in vitro

. ,

Bacillus cereus 76 79, Serratia marcescens 5-, 47 52, Ser-

ratia rubidea 55 49, Pseudomonas chlororaphisToZ7

9-12, 1-5-10, 4-8, -

Verticillium dahliae, Sclerotium rolfsii, Rhizoctoniasolani,

3 Forl. , 76, 5-

, ToZ7, ToZ9-12 ToZ4-8 in vitro Forl S.

rolfsii .

(11)

S. rolfsii, 5 -

.

in vitro ,

. S. rolfsii 5- 47 .

, 5-, ToZ7, 4-8 9-

12 ,

, 76 3 .

, V. dahliae 52 ,

, -

.

168 16o


169/192

mhnj{bfrf sfq do_p`rfq jjflhi}l onknl}rdxl snt b]lntq #+- rs` ahnjnbhi\ v`p`isfphrshi\ l\ostmfq uts}l p}lh`q (A96)3)6+)97))

.. Uubvifw, Y. Ycryxtz, U. yxntz, _. Ufrivbw, . Utzytpl, . \xfrfoyxeitz,. Yfpbytw ofn [. [yj{frtz

, 57400

Aronia melanocarpa , , -

. -

Trichoderma, Trichoderma harzianum Trichoderma as-

perellum,

. ,

,

, 58%, 2

, 94%, 30 . -

, -

, 80, 87, 94 95% , 2,

7, 15 30 , . , -

. -

Trichoderma harzianum B2

B3 ,

T. asperellum B1

, . ,

-

Aronia melanocarpa .

169


170/192

onk{lxrf i`h knph`i{q v`p`isfphrk{q dohutshi}l etk}l `ko]jnti`h `mhnj{bfrf sfq `ls`bxlhrshi^q sntq p\rfq dl`ls_nl kti^sxl sntb]lntq A:79/33


171/192

hnjnbhi^ lshkds}ohrf snt k|ifs` A:79/33


172/192

mhnj{bfrf sxl ahnjnbhi}l o`p`b{lsxl )*)+3


173/192

jdbvnq dbi`s\rs`rfq i`h dm\ojxrfq tono`gnb{lxl rsdjdv}l sntk|ifs` c9@706+;9) 7)9):;+) kds\ sfl du`pknb^ ahnjnbhi^q i`s`-onj]kfrfq rsfl Udphu]pdh` od_pnt

.Q. \kevtw, _. Yjvpcvtz, N. \tufpeitz, . _vnxyutzptw ofn [. nfqfrydw , , 57006 ,

Cry-phonectria parasitica 1963. 2002 ,

2006 .

Cryphonectria hypovirus (CHV),

. -

(hv),

2007-2009 (-

(..) ) -

.

..

.. . -

/ -

PDA . .. 34,9 - 53,1% , ..

39,2 68,5%. , 35,9 61,8%

, .. 32,3 - 48,8%. -

.

173


174/192

Upnrhnphrk{q snt \phrsnt vp{lnt o`p`knl^q sxl rijfpxs_xl rsflo\rs` v}k`snq i`s\ sfl onk{lxrf ktino`p`r_sxl sxl rijfpxs_xlsnt utsno`gnb{lnt `rink|ifs` "+396;) :+396;69


175/192

o`pntr_` ktino`p`r_sxl sxl rijfpxs_xl snt utsno`gnb{lnt`rink|ifs` "+396;) :+396;69


176/192

XTXSTPZaUNVP JV\RUN\aYR[P

mhnj{bfrf dohu`ldhp`rshi}l ntrh}l rsfl dldpbnon_frf snt db-bdln|q `lnrnonhfshin| rtrs^k`snq snt utsn| A9)*,67:: ;0)3))

^. hhjptutptz1, Y.Y. ry~retz1, N.S. \kbqtw1, J. K>2 ofn .R. \xnyxnhnbrrlw1

1. , , -

, 75, 118 55

2. ex. EmeritusProfessor, 5502 Lorna St., Torrance, CA 90503, USA

Verticillium dahliae -

Pseudomonas syringaepv tomato,

.

,

.

,

. ,

-

(,B,C,D,E)

. Arabidopsis

thaliana V. dahliae Pseudomonas

syringae pv. tomato -

, -

. V. dahliae, D

10mM -

20%. P. syringae pv. tomato -

. ,

C D ( 10 mM) -

, PR-1 PR-5

( Pathogen Related Proteins) A. thaliana.

176 16o


177/192

C3 ;37C;9D G


178/192

*@;=M599 G 7=:G


179/192

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Renovation Sekamosa Verticillium dahliae

. , -

in vitro . ,

-

in vitro EC50 0,025-0,05

%. in planta ,

( AUDPC)

, -

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-

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Renovation Sekamosa

.

179


180/192

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181/192

G5G33


182/192

*7;HC;3?;7OB;9 73-H57?O? B3:5M?K? 3 BKC


183/192

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184/192


185/192

. 172

. 108

. 176, 178

.. 74, 165

. 121

. 144

.. 122, 156

. 79

.. 180

. 123

.. 38, 103, 166, 176, 182

.. 165

.. 143

. 73

. 148

.. 40 . 32, 69, 88, 102, 141, 152

. 74, 167

. 69, 93, 102

.. 58

. 54, 55, 56, 158

. 166

.. 26, 36, 43, 49, 138

. 74, 167

. 179

. 105

. 28, 177

. 147

. 41

.. 75

. 91

. 105

. 124 . 74, 165

. 89

.. 48

. 103

. 74, 165

. 142

.. 48

. 28, 177

.. 26, 36, 43, 49, 138

.. 139, 140

. 180

. 148

.. 141

. 188

.. 83

. 99

. 159, 164, 171

. 149 . 85

. 88

. 166

. 133, 134, 136, 173

. 121

. 147

. 149

.. 81, 82, 151

. 169

.. 75

. 99, 143, 157

.. 33, 83

. 134

. 28, 177

.. 137

. 126

.. 81, 82, 84, 142, 151 . 108


186/192

. 178

.. 60, 75

. 125

. 67

. 105, 126

. 35, 42, 73, 155, 170

. 183

. 47

. 71

.. 77, 78

. 91, 144

. 127

. 55, 56

. 102

. 137 .. 45

.. 110

. 34, 35, 42, 155

- . 150

. 143

. 87

. 91

.. 77

. 121

. 154

.. 41, 54, 55, 56, 58, 67,

121, 128, 158, 163

.. 139, 140

. 46

.. 160

.. 33, 81, 82, 83, 84, 85, 86, 137, 142,

145, 146, 148, 149, 151, 153

. 166

. 91 .. 33, 83, 84, 142

. 93

. 66, 67, 69, 163

. 163

. 67

.. 45, 182 . 127

. 88, 141, 143

. 105

. 97

. 144

. 83

. 150

. 154

. 128

. 67

. 178

.. 107, 110, 129, 168

.. 52

.. 87, 94, 95

. 70

.. 39, 40, 130, 131, 132,

179, 180 . 63

. 110, 137, 142, 145, 146, 149

. 98, 124

.. 94

. 147

. 69, 88

.. 52, 60, 77, 78

. 168

.. 33, 81, 82, 83, 84, 85, 92,

137, 148, 149, 153

. 87

. 169

. 154

. 88

. 154

.. 39, 40, 130, 131, 132, 179,

180 .. 52, 56, 58, 60, 75, 77, 78

186 16o


187/192

. 110

- . 100, 121, 160

. 134

. 148

. 108, 169 .. 36, 43, 138

.. 108, 169

. 183

.. 143

. 159

. 66, 69

.. 66, 67, 163

.. 101

. 91

. 63

.. 133, 134

. 172

- . 98

. 129

. 143

. 100

. 91

. 154

.. 85, 145, 148, 149

.. 26

. 27

. 108, 169

.. 49

- . 105

. 99, 157

.. 34, 46, 73, 155, 170

. 65

. 41, 67, 163

.. 85, 86, 149, 150

. 166

.. 160

. 181 .. 40, 130, 131, 132

. 166

. 161

.. 46, 47, 74, 109, 127, 171,

172, 182

. 46 .. 53, 70, 151

.. 81, 82, 142, 151, 153

. 63

. 86

.. 43, 138

. 169

. 133, 134, 136, 173

. 35

.. 39

.. 135

.. 135

. 79

. 28, 177

.. 26, 36, 43, 49, 138

.. 78 . 66

.. 26, 28, 177

. 69

. 171

. 27

. 88

. 65

. 89

.. 26

. 69

. 108, 169

.. 47

. 41

.. 38, 103, 176, 182

.. 46, 109, 127, 171, 172, 182

. 69, 88, 93, 141, 152

. 108 . 98

187


188/192

.. 136, 173

. 91

. 88

. 173

.. 26, 36, 43, 49, 138

. 154

. 66

. 34, 35, 42, 73, 79, 155, 170

. 108, 169

.. 111, 174, 175

. 124

.. 137, 146, 163

. 87

. 42 . 164

. 28

.. 45, 47, 48, 74, 123, 125,

159, 165, 167, 176, 178, 182

-. 34

. 24, 27

.. 40, 130, 131, 132

. 105 . 127

. 152

. 180

.. 132

. 129

.. 153

. 109

.. 89, 164

. 67

. 53, 70, 161

.. 139, 140

. 183

. 170

. 173

.. 141

. 155

Afifi H. 87

Baltrus D.A. 49

Berner D.K. 107

Bertolini E. 32

Bull C.T. 49

Bergey B. 30

C

Calvo M. 92

Cambra M. 30, 32

Candresse T. 30, 32Carbonell A. 92

Cotty P.J. 74

D

Dangl J.L. 49

F

Faure C. 30

G

Garcia J.A. 92

Gazivoda A. 87

Genet J.L. 65

H

Hahn M. 54

Hosokawa M. 101

J

Jones C.D. 49

Jones J.D.G. 45

K

Kageshima S. 71

Kashefi J. 107, 129

Kimura N. 162

Klappach K. 62

Kuc J. 176

188 16o


189/192

L

Leroch M. 54

Laizet Y. 30

M

Manevski K. 87

Martinez M.C. 32

Matysiak R. 65

Meier-Runge F. 63

Moury B. 93

Marais A. 30

O

Olmos A. 30, 32Olsthorn R.C.L. 95

Osman T.A.M. 95

S

Senechal Y. 162

Shegani M. 87

Simon V. 93

Stammler G. 62

Svanella-Dumas L. 30

T

Tanaka S. 162

Terry L.A. 102

V

Valli A. 92

van Leeuwen G.C.M. 28

Velimirovic A. 87

Vinatzer B.A. 49

W

Wechter W.P. 49

Y

Yan S. 49

189


190/192


191/192

+962


192/192

16th congress in plant pathology

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